Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Stem Cells Translational Medicine

Volumn 2, Issue 4, 2013, Pages 246-254

  Kunova, Michaela ^a   Matulka, Kamil ^a   Eiselleova, Livia ^a   Salykin, Anton ^a,b   Kubikova, Iva ^a   Kyrylenko, Sergiy ^a   Hampl, Ales ^a,b   Dvorak, Petr ^a,b  

^a MASARYK UNIVERSITY   (Czech Republic)

^b ST ANNE S UNIVERSITY HOSPITAL   (Czech Republic)

Author keywords

Cell culture and bull; Cell survival and bull; Embryonic stem cells and bull; Induced pluripotent stem cells; Xenograft assay and bull

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL) CYCLOHEXANECARBOXAMIDE; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN BCL 2; PROTEIN BCL XL; STAGE SPECIFIC EMBRYO ANTIGEN; STAGE SPECIFIC EMBRYO ANTIGEN 3; STAGE SPECIFIC EMBRYO ANTIGEN 5; SURVIVIN; TRANSCRIPTION FACTOR NANOG; UNCLASSIFIED DRUG;

ADAPTATION; ANIMAL EXPERIMENT; ARTICLE; CELL CLONE; CELL DEATH; CELL DIFFERENTIATION; CELL SURVIVAL; CELL SUSPENSION; CELL VIABILITY; COLONY FORMING UNIT; COMPARATIVE STUDY; EMBRYOID BODY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; KARYOTYPING; MONOLAYER CULTURE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TERATOMA; WESTERN BLOTTING;

EID: 84876228247     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2012-0081     Document Type: Article

References (35)

1. Mummery CL. Cardiology: Solace for the broken-hearted? Nature 2005;433:585-587.
2. Desbordes SC, Placantonakis DG, Socci A et al. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell 2008;2:602-612.
3. Steiner D, Khaner H, Cohen M et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol 2010;28:361-364.
4. Zweigerdt R, Olmer R, Singh H et al. Scalable expansion of human pluripotent stem cell in suspension culture. Nat Protoc 2011;6:689- 700.
5. Amit M, Laevsky I, Miropolsky Y et al. Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells. Nat Protoc 2011;6:572-579.
6. Olmer R, Lange A, Selzer S et al. Suspension culture of human pluripotent stem cells in controlled, stirred bioreactors. Tissue Eng Part C Methods 2012;18:772-784.
7. Hasegawa K, Fujioka T, Nakamura Y et al. A method for the selection of human embryonic stem cell sublines with gigh replating efficiency after single-cell dissociation. STEM CELLS 2006; 24:2649-2660.
8. Ellerström C, Strehl R, Noaksson K et al. Facilitated expansion of human embryonic stem cells by single-cell enzymatic dissociation. STEM CELLS 2007;25:1690-1696.
9. Bajpai R, Lesperance J, Kim M et al. Efficient propagation of single cells Accutase-dissociated human embryonic stem cells. Mol Reprod Dev 2008;75:818-827.
10. Watanabe K, Ueno M, Kamiya D et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 2007;25:681-686.
11. Claassen DA, Desler MM, Rizzino A. ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells. Mol Reprod Dev 2009;76:722-732.
12. Singh H, Mok P, Balakrishnan T et al. Upscaling single cell-inoculated suspension cul ture of human embryonic stem cells. Stem Cell Res 2010;4:165-179.
13. Ungrin MD, Loshi C, Nica A et al. Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One 2008;13:e1565.
14. Braam SR, Denning C, Matsa E et al. Feederfree culture of human embryonic stem cells in conditioned medium for efficient genetic modification. Nat Protoc 2008;3:1435-1443.
15. Kunova M, Matulka K, Eiselleova L et al. Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells. Reprod Biomed Online 2010;21:676-686.
16. Peerani R, Onishi K, Mahdavi A et al. Manipulation of signaling thresholds in engineered stem cells niches identifies design criteria for pluripotent stem cell screens. PLoS One 2009;4:e6438.
17. Hough SR, Laslett AL, Grimmond SB et al. A continuum of cell states spans pluripotency and lineage commitment in human embryonic stem cells. PLoS One 2009;4:e7708.
18. Chen G, Hou Z, Gulbranson DR et al. Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells. Cell Stem Cell 2010;7: 240-248.
19. Tang C, Lee AS, Volkmer JP et al. An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Nat Biotechnol 2011;29:829-834.
20. Pomper MG, Hammond H, Yu X et al. Serial imaging of human embryonic stem-cell engraftment and teratoma formation in live mouse models. Cell Res 2009;19:370-379.
21. Ardehali R, Inlay MA, Ali SR et al. Overexpression of BCL2 enhances survival of human embryonic stem cells during stress and obviates the requirement for serum factors. Proc Natl Acad Sci USA 2011;108:3282- 3287.
22. Amps K, Andrews PW, Anyfantis G et al. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nat Biotechnol 2011;29: 1132-1144.
23. Bai H, Chen K, Gao YX et al. Bcl-xL enhances single-cell survival and expansion of human embryonic stem cells without affecting self-renewal. Stem Cell Res 2012;8:26-37.
24. Blum B, Bar-Nur O, Golan-Lev T et al. The anti-apoptotic gene survivin contributes to teratoma formation by human embryonic stem cells. Nat Biotechnol 2009;27:281-287.
25. Amit M, Carpenter MK, Inokuma MS et al. Clonally derived human embryonic stem cell lines remain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 2000;227:271-278.
26. Ohgushi M, Matsumura M, Eiraku M et al. Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells. Cell Stem Cell 2010;7:225-239.
27. Li L, Wang BH, Wang S et al. Individual cell movement, asymmetric colony expansion, rho-associated kinase, and E-cadherin impact the clonogenicity of human embryonic stem cells. Biophys J 2010;98:2242-2251.
28. Närvä E, Autio R, Rahkonen N et al. Highresolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity. Nat Biotechnol 2010;28:371-377.
29. Tonge PD, Shigeta M, Schroeder T et al. Functionally defined substrates within the human embryonic stem cell compartment. Stem Cell Res 2011;7:145-153.
30. Enver T, Soneji S, Joshi C et al. Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells. Hum Mol Genet 2005;14:3129-3140.
31. Stewart MH, Bossé M, Chadwick K et al. Clonal isolation of hESCs reveals heterogeneity within the pluripotent stem cell compartment. Nat Methods 2006;3:807-815.
32. Song Y, Withers DA, Hakomori S. Globoside-dependent adhesion of human embryonal carcinoma cells, based on carbohydratecarbohydrate interaction, initiates signal transduction and induces enhanced activity of transcription factors AP1 and CREB. J Biol Chem 1998;273:2517-2525.
33. Xu Y, Zhu X, Hahm HS et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 2010; 107:8129-8134.
34. Werbowetski-Ogilvie TE, Bossé M,Stewart M et al. Characterization of human embryonic stem cells with features of neoplastic progression. Nat Biotechnol 2009;27: 91-97.
35. Rodin S, Domogatskaya AStröm Setal, Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat Biotechnol 2010;28:611-615.