Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection

Journal of Immunological Methods

Volumn 391, Issue 1-2, 2013, Pages 174-178

  Yang, Hongbing ^a   Yorke, Elisabeth ^b   Hancock, Gemma ^a   Clutton, Genevieve ^b   Sande, Nellia ^c   Angus, Brian ^b   Smyth, Redmond ^d   Mak, Johnson ^e   Dorrell, Lucy ^a,b,c  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c OXFORD UNIVERSITY HOSPITALS NHS FOUNDATION TRUST   (United Kingdom)

^d UNIVERSITÉ DE STRASBOURG   (France)

^e DEAKIN UNIVERSITY   (Australia)

Author keywords

CD4 T cell; ELISA; Flow cytometry; Gag; HIV; P24 Ag

Indexed keywords

ANTIGEN P24; METHANOL; PARAFORMALDEHYDE; TRITON X 114;

ANTIGEN DETECTION; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; NONHUMAN; PRIORITY JOURNAL;

BIOLOGICAL MARKERS; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CELLS, CULTURED; DETERGENTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIXATIVES; FLOW CYTOMETRY; FORMALDEHYDE; HIV CORE PROTEIN P24; HIV INFECTIONS; HIV-1; HUMANS; METHANOL; POLYETHYLENE GLYCOLS; POLYMERS; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TISSUE FIXATION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84876141325     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.03.001     Document Type: Article

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