Comparison of photostability and photobleaching properties of FITC- and Dylight488- conjugated Herceptin

International Journal of Green Nanotechnology: Biomedicine

Volumn 3, Issue 4, 2011, Pages 264-270

  Mahmoudi, Ahmad Reza ^a   Shaban, Elham ^a   Ghods, Roya ^a   Jeddi Tehrani, Mahmood ^a   Emami, Shaghayegh ^a   Rabbani, Hodjattallah ^a,b   Zarnani, Amir Hassan ^a   Mahmoudian, Jafar ^a  

^a AVICENNA RESEARCH INSTITUTE   (Iran)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Dylight488; FITC (fluorescein isothiocyanate); Mean fluorescence intensity (MFI); Photobleaching; Photostability

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; TRASTUZUMAB;

ARTICLE; BLEACHING; DRUG CONJUGATION; FLOW CYTOMETRY; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; SPECTROPHOTOMETER;

EID: 84875992062     PISSN: 1943085X     EISSN: 19430906     Source Type: Journal    
DOI: 10.1080/19430892.2011.633480     Document Type: Article

References (24)

1. Banks, P. R.; Paquette, D. M. Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis. Bioconjug. Chem. 1995, 6 (4), 447-458.
2. Giepmans, B. N.; Adams, S. R.; Ellisman, M. H.; Tsien, R. Y. The fluorescent toolbox for assessing protein location and function. Science 2006, 312 (5771), 217-224.
3. Adams, K. E.; Ke, S.; Kwon, S.; Liang, F.; Fan, Z.; Lu, Y.; Hirschi, K.; Mawad, M. E.; Barry, M. A.; Sevickuraca, E. M. Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer. J. Biomed. Opt. 2007, 12 (2), 024017.
4. Berlier, J. E.; Rothe, A.; Buller, G.; Bradford, J.; Gray, D. R.; Filanoski, B. J.; Telford, W. G.; Yue, S.; Liu, J.; Cheung, C.Y.; Chang, W.;Hirsoh, J. D.; Beechem, J.M.; Haugland, R. P.; Haugland, R. P. Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates. J. Histochem. Cytochem. 2003, 51 (12), 1699-1712.
5. Gruber, H. J.; Hahn, C. D.; Kada, G.; Riener, C. K.; Harms, G. S.; Ahrer, W.; Dax, T. G.; Knaus, H. G. Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Bioconjug. Chem. 2000, 11 (5), 696-704.
6. Hahn, C. D.; Riener, C. K.; Gruber, H. J. Labeling of antibodies with Cy3-, Cy3. 5-, Cy5-, and Cy5. 5-monofunctional dyes at defined dye/protein ratios. Single Molecules 2001, 2 (2), 149-153.
7. Hama, Y.; Urano, Y.; Koyama, Y.; Bernardo, M.; Choyke, P. L.; Kobayashi, H. A comparison of the emission efficiency of four common green fluorescence dyes after internalization into cancer cells. Bioconjug. Chem. 2006, 17 (6), 1426-1431.
8. Panchuk-Voloshina, N.; Haugland, R. P.; Bishop-Stewart, J.; Bhalgat, M. K.; Millard, P. J.; Mao, F.; Leung, W. Y.; Haugland, R. P. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J. Histochem. Cytochem. 1999, 47 (9), 1179-1188.
9. Holmes, K. L.; Lantz, L. M. Protein labeling with fluorescent probes. Methods Cell Biol. 2001, 63, 185-204.
10. Ahmed, S. M.; Daulat, A. M.; Meunier, A.; Angers, S. G. Protein betagamma subunits regulate cell adhesion through Rap1a and its effector Radil. J. Biol. Chem. 2010, 285 (9), 6538-6551.
11. Bechler, M. E.; Doody, A. M.; Racoosin, E.; Lin, L.; Lee, K. H.; Brown, W. J. The phospholipase complex PAFAHIb regulates the functional organization of the Golgi complex. J. Cell. Biol. 2010, 190 (1), 45-53.
12. Geoghegan, K. F.; Stroh, J. G. Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine. Bioconjug. Chem. 1992, 3 (2), 138-146.
13. Chen, A. K.; Cheng, Z.; Behlke, M. A.; Tsourkas, A. Assessing the sensitivity of commercially available fluorophores to the intracellular environment. Anal. Chem. 2008, 80 (19), 7437-7444.
14. Haugland, R. P. Coupling of monoclonal antibodies with enzymes. Methods Mol. Biol. 1995, 45, 235-243.
15. Deshpande, A. V.; Beidoun, A.; Penzkofer, G. Spectroscopic investigation of cyanovinyl-diethylaniline dyes in solutions. Chem. Physics 1990, 148 (1), 141-154.
16. Resch-Genger, U.; Grabolle, M.; Cavaliere-Jaricot, S.; Nitschke, R.; Nann, T. Quantum dots versus organic dyes as fluorescent labels. Nature Methods 2008, 5 (9), 763-775.
17. Randolph, J. B.; Waggoner, A. S. Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes. Nucleic Acids Res. 1997, 25 (14), 2923-2929.
18. Gruber, H. J.; Hahn, C. D.; Kada, G.; Riener, C. K.; Harms, G. S.; Ahrer, W.; Dax, T. G.; Knaus, H. G. Anomalous fluorescence enhancement of Cy3 and Cy3. 5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Bioconjug. Chem. 2000, 11 (5), 696-704.
19. Li, D. H.; Zhu, Q. Z.; Ye, D.; Fang, Y.; Xu, J. G. A rapid method for the determination of molar ratio of fluorophore to protein by fluorescence anisotropy detection. Anal. Chim. Acta 1999, 389 (1-3), 85-88.
20. Lim, C. S.; Miller, J. N.; Bridges, J. W. Energytransfer immunoassay: a study of the experimental parameters in an assay for human serum albumin. Anal. Biochem. 1980, 108 (1), 176-184.
21. Hermanson, G. T. Bioconjugate Techniques, Second edn. Illinois: Elsevier Inc., 2008.
22. Randolph, J. B.; Waggoner, A. S. Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes. Nucleic Acids Res. 1997, 25 (14), 2923.
23. Schmidt, T.; Kubitscheck, U.; Rohler, D.; Nienhaus, U. Photostability data for fluorescent dyes: an update. Single Molecules 2002, 3 (5-6), 327.
24. * 1. Anal. Biochem. 1987, 161 (2), 442-452.