Short-term storage does not affect the quantitative yield of mycobacterium tuberculosis in sputum in early-bactericidal-activity studies

Journal of Clinical Microbiology

Volumn 51, Issue 4, 2013, Pages 1094-1098

  Kolwijck, Eva ^a,b   Mitchell, Melissa ^a,c   Venter, Amour ^a   Friedrich, Sven O ^a   Dawson, Rod ^d   Diacon, Andreas H ^a,c  

^a UNIVERSITY OF STELLENBOSCH   (South Africa)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^c Karl Bremer Hospital   (South Africa)

^d UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

TUBERCULOSTATIC AGENT;

ARTICLE; BACTERIAL LOAD; BACTERIAL VIABILITY; BACTERICIDAL ACTIVITY; COLONY FORMING UNIT; CONTAMINATION; EARLY BACTERICIDAL ACTIVITY; FREEZING; LABORATORY DIAGNOSIS; MICROBIOLOGICAL PARAMETERS; MYCOBACTERIUM TUBERCULOSIS; PRESERVATION AND STORAGE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPUTUM CULTURE; SPUTUM SMEAR; SPUTUM STORAGE; TIME TO CULTURE POSITIVITY;

BACTERIAL LOAD; BACTERIOLOGICAL TECHNIQUES; COLONY COUNT, MICROBIAL; HUMANS; MICROBIAL VIABILITY; MYCOBACTERIUM TUBERCULOSIS; REFRIGERATION; SPECIMEN HANDLING; SPUTUM; TIME FACTORS; TUBERCULOSIS;

MYCOBACTERIUM TUBERCULOSIS;

EID: 84875857148     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02751-12     Document Type: Article

References (15)

1. Bhat J, Selvakumar N, Rao VG, Gopi PG, Yadav R, Wares DF. 2011. Yield of culture of Mycobacterium tuberculosis complex in sputum samples transported from tribal areas. Int. J. Tuberc. Lung Dis. 15:478-482.
2. Banda HT, Harries AD, Boeree MJ, Nyirenda TE, Banerjee A, Salaniponi FM. 2000. Viability of stored sputum specimens for smear microscopy and culture. Int. J. Tuberc. Lung Dis. 4:272-274.
3. Rao KP, Nair SS, Cobbold N, Naganathan N. 1966. Some operational factors influencing the utility of culture examination in the diagnosis of pulmonary tuberculosis. Bull. World Health Organ. 34:589-604.
4. Sula L, Sundaresan TK, Langerova M. 1960. Effects of storage and transport on the cultivability of mycobacteria. Bull. World Health Organ. 23:635-651.
5. Paramasivan CN, Narayana AS, Prabhakar R, Rajagopal MS, Somasundaram PR, Tripathy SP. 1983. Effect of storage of sputum specimens at room temperature on smear and culture results. Tubercle 64:119-124.
6. Piersimoni C, Scarparo C, Callegaro A, Tosi CP, Nista D, Bornigia S, Scagnelli M, Rigon A, Ruggiero G, Goglio A. 2001. Comparison of MB/ BacT ALERT 3D system with radiometric BACTEC system and Löwenstein-Jensen medium for recovery and identification of mycobacteria from clinical specimens: A multicenter study. J. Clin. Microbiol. 39:651-657.
7. Tessema B, Beer J, Emmrich F, Sack U, Rodloff AC. 2011. Rate of recovery of Mycobacterium tuberculosis from frozen acid-fast-bacillus smear-positive sputum samples subjected to long-term storage in Northwest Ethiopia. J. Clin. Microbiol. 49:2557-2561.
8. Diacon AH, Maritz JS, Venter A, van Helden PD, Dawson R, Donald PR. 2012. Time to liquid culture positivity can substitute for colony counting on agar plates in early bactericidal activity studies of antituberculosis agents. Clin. Microbiol. Infect. 18:711-717.
9. Hesseling AC, Walzl G, Enarson DA, Carroll NM, Duncan K, Lukey PT, Lombard C, Donald PR, Lawrence KA, Gie RP, van Helden PD, Beyers N. 2010. Baseline sputum time to detection predicts month two culture conversion and relapse in non-HIV-infected patients. Int. J. Tuberc. Lung Dis. 14:560-570.
10. Visser ME, Stead MC, Walzl G, Warren R, Schomaker M, Grewal HM, Swart EC, Maartens G. 2012. Baseline predictors of sputum culture conversion in pulmonary tuberculosis: Importance of cavities, smoking, time to detection and W-Beijing genotype. PLoS One 7:e29588. doi:10.1371/journal.pone.0029588.
11. Donald PR, Diacon AH. 2008. The early bactericidal activity of antituberculosis drugs: A literature review. Tuberculosis (Edinb) 88(Suppl 1): S75-S83.
12. Hafner R, Cohn JA, Wright DJ, Dunlap NE, Egorin MJ, Enama ME, Muth K, Peloquin CA, Mor N, Heifets LB. 1997. Early bactericidal activity of isoniazid in pulmonary tuberculosis. Optimization of methodology. The DATRI 008 Study Group. Am. J. Respir. Crit. Care Med. 156(Pt 1):918-923.
13. World Health Organization. 1998. Laboratory services in tuberculosis control. (WHO/tuberculosis/98.258). WHO, Geneva, Switzerland. http://www.who.int/tb/dots/lab.pdf.
14. Jonckheere AR. 1954. A distribution-free k-sample test against ordered alternatives. Biometrika 41:133-145.
15. Diacon AH, Dawson R, du Bois J, Narunsky K, Venter A, Donald PR, van Niekerk C, Erondu N, Ginsberg AM, Becker P, Spigelman MK. 2012. Phase II dose-ranging trial of the early bactericidal activity of PA-824. Antimicrob. Agents Chemother. 56:3027-3031.