Reply to Campos and Muñoz: Why phosphate is a bad buffer for guanidinium chloride titrations

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 14, 2013, Pages

  Fersht, Alan R ^a   Petrovich, Miriana ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINE; BUFFER; GUANIDINE; PHOSPHATE; SODIUM CHLORIDE;

IONIZATION; LETTER; PH; PRIORITY JOURNAL; PROTON TRANSPORT; TITRIMETRY;

ESCHERICHIA COLI PROTEINS; FLUORESCENCE RESONANCE ENERGY TRANSFER; PROTEIN FOLDING;

EID: 84875833278     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1303286110     Document Type: Letter

References (7)

1. Liu J, et al. (2012) Exploring one-state downhill protein folding in single molecules. Proc Natl Acad Sci USA 109(1):179-184.
2. Campos LA, Muñoz V (2013) Reply to Huang et al.: Slow proton exchange can duplicate the number of species observed in single molecule experiments of protein folding. Proc Natl Acad Sci USA 110:E1242-E1243.
3. Huang F, Johnson CM, Petrovich M, Fersht AR (2013) Don't waste good methods on bad buffers and ambiguous data. Proc Natl Acad Sci USA 110(5): E331-E332.
4. Garcia-Mira MM, Sanchez-Ruiz JM (2001) pH corrections and protein ionization in water/guanidinium chloride. Biophys J 81(6): 3489-3502.
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7. Fersht A (1998) Structure and Mechanism in Protein Science (WH Freeman, New York), pp 162-163.