pH corrections and protein ionization in water/guanidinium chloride

Biophysical Journal

Volumn 81, Issue 6, 2001, Pages 3489-3502

  Garcia Mira, M M ^a   Sanchez Ruiz, J M ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLIC ACID DERIVATIVE; CHYMOTRYPSIN A; GUANIDINE; NUCLEASE; RIBONUCLEASE A; SODIUM CHLORIDE; WATER;

ARTICLE; ENERGY; IONIZATION; PH; PH ELECTRODE; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTON TRANSPORT;

EID: 0035193201     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75980-2     Document Type: Article

References (33)

1. Protein stability curves
2. Effects of guanidinium chloride hydrochloride on the proton inventory of proteins: Implications on interpretations of protein stability
3. Thermodynamic constants for tautomerism, hydration, and ionization of vitamin B6 compounds in water/dioxane
4. Thermodynamic analysis of interactions between denaturants and protein surface exposed on unfolding: Interpretation of urea and guanidinium chloride m-values and their correlation with changes in accessible surface area (ASA) using preferential interaction coefficients and the local-bulk domain model
5. Urea and guanidinium chloride hydrochloride denaturation of ribonuclease, lysozyme, alphachymotrypsin, and beta-lactoglobulin
6. The acidity and basicity scale in the system ethanol-water. The evaluation of degenerate activity coefficients for single ions
7. Guanidinium chloride hydrochloride-induced folding of proteins
8. Thermal versus guanidinium chloride-induced unfolding of ubiquitin. An analysis in terms of the contributions from charge-charge interactions to protein stability
9. A model-independent, nonlinear extrapolation procedure for the characterization of protein folding energetics from solvent-denaturation data
10. The effect of sodium chloride upon the ionization of glycine
11. Thermodynamics of protein interactions with urea and guanidinium chloride
12. Thermodynamics of aqueous guanidinium hydrochloride solutions in the temperature range from 283.15 to 313.15 K
13. Anion binding to the ubiquitin molecule
14. Stabilization of a protein by guanidinium chloride
15. Protein denaturation with guanidinium chloride hydrochloride or urea provides a different estimate of stability depending on the contributions of electrostatic interactions
16. Acid-base titrations in concentrated guanidinium chloride hydrochloride. Dissociation constants of the guanidinium ion and of some amino acids
17. Proteins as random coils. II. Hydrogen ion titration curve of ribonuclease in 6 M guanidinium chloride hydrochloride
18. Determination and analysis of urea and guanidinium chloride hydrochloride denaturation curves
19. pH dependence of the urea and guanidinium chloride hydrochloride denaturation of ribonuclease A and ribonuclease T1
20. Thermodynamic constants for tautomerism and ionization of pyridoxine and 3-hydroxypyridine in water-dioxane
21. Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants
22. A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range
23. Theory of protein titration curves. I. General equations for impenetrable spheres
24. Determination of thermodynamic equilibrium constants in mixed solvents
25. Studies on coordination compounds. I. A method for determining thermodynamics equilibrium constants in mixed solvents
26. pH dependence of stability of staphylococcal nuclease: Evidence of substantial electrostatic interactions in the denatured state
27. How valid are denaturant-induced unfolding free measurements? Level of conformance to common assumptions over an extended range of ribonuclease A stability