Expression, purification, characterization, and solution nuclear magnetic resonance study of highly deuterated yeast cytochrome c peroxidase with enhanced solubility

Biochemistry

Volumn 52, Issue 13, 2013, Pages 2165-2175

  Volkov, Alexander N ^a,b   Wohlkonig, Alexandre ^b   Soror, Sameh H ^b,c   Van Nuland, Nico A J ^a,b  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c Helwan   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPHYSICAL CHARACTERIZATION; BIOPHYSICAL TECHNIQUES; DEUTERIUM INCORPORATIONS; ESCHERICHIA COLI EXPRESSION SYSTEMS; HIGH-AFFINITY BINDING SITES; NUCLEAR MAGNETIC RESONANCE STUDIES; NUCLEAR MAGNETIC RESONANCE(NMR); PURIFICATION PROCEDURES;

DEUTERIUM; ESCHERICHIA COLI; NUCLEAR MAGNETIC RESONANCE; PROTEINS; PURIFICATION; SOLUBILITY; X RAY CRYSTALLOGRAPHY; YEAST;

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

CYTOCHROME C PEROXIDASE; DEUTERIUM; POLYHISTIDINE TAG;

ARTICLE; BACTERIUM CULTURE; BINDING AFFINITY; BINDING SITE; CARBON NUCLEAR MAGNETIC RESONANCE; CARBOXY TERMINAL SEQUENCE; DEUTERON NUCLEAR MAGNETIC RESONANCE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; NITROGEN NUCLEAR MAGNETIC RESONANCE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOLUBILITY; X RAY CRYSTALLOGRAPHY; YEAST;

AMINO ACID SEQUENCE; CIRCULAR DICHROISM; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME-C PEROXIDASE; CYTOCHROMES C; ESCHERICHIA COLI; GENE EXPRESSION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SOLUBILITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84875802862     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi400220w     Document Type: Article

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