BDE99 (2,2',4,4',5-pentabromodiphenyl ether) suppresses differentiation into neurotransmitter phenotypes in PC12 cells

Neurotoxicology and Teratology

Volumn 37, Issue , 2013, Pages 13-17

  Slotkin, Theodore A ^a   Card, Jennifer ^a   Infante, Alice ^a   Seidler, Frederic J ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Acetylcholine; BDE99; Brominated flame retardants; Dopamine; Neurodifferentiation; PC12 cells

Indexed keywords

2,2',4,4',5 PENTABROMODIPHENYL ETHER; ACETYLCHOLINE; CHLORPYRIFOS; CHOLINE ACETYLTRANSFERASE; DIPHENYL ETHER DERIVATIVE; DOPAMINE; NEUROTRANSMITTER; TYROSINE 3 MONOOXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; CELL ASSAY; CELL COUNT; CELL ENLARGEMENT; CELL FATE; CELL LOSS; CELLULAR PARAMETERS; CHOLINERGIC SYSTEM; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DNA CONTENT; DOPAMINERGIC SYSTEM; NERVE CELL DIFFERENTIATION; NERVE FIBER GROWTH; NERVOUS SYSTEM DEVELOPMENT; NEUROTOXICITY; OXIDATIVE STRESS; PC12 CELL; PHENOTYPE; PRIORITY JOURNAL; TARGET CELL; TOTAL PROTEIN DNA RATIO;

ACETYLCHOLINE; ANIMALS; CELL COUNT; CELL DIFFERENTIATION; CHOLINE O-ACETYLTRANSFERASE; DOPAMINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENVIRONMENTAL POLLUTANTS; HALOGENATED DIPHENYL ETHERS; NEUROTRANSMITTER AGENTS; PC12 CELLS; PHENOTYPE; RATS; TYROSINE 3-MONOOXYGENASE;

EID: 84875659827     PISSN: 08920362     EISSN: 18729738     Source Type: Journal    
DOI: 10.1016/j.ntt.2013.02.001     Document Type: Article

References (42)

1. Axelrad J.C., Howard C.V., McLean W.G. The effects of acute pesticide exposure on neuroblastoma cells chronically exposed to diazinon. Toxicology 2003, 185:67-78.
2. Belles M., Alonso V., Linares V., Albina M.L., Sirvent J.J., Domingo J.L., et al. Behavioral effects and oxidative status in brain regions of adult rats exposed to BDE-99. Toxicol. Lett. 2010, 194:1-7.
3. Blanco J., Mulero M., Lopez M., Domingo J.L., Sanchez D.J. BDE-99 deregulates BDNF, Bcl-2 and the mRNA expression of thyroid receptor isoforms in rat cerebellar granular neurons. Toxicology 2011, 290:305-311.
4. Branchi I., Capone F., Alleva E., Costa L.G. Polybrominated diphenyl ethers: neurobehavioral effects following developmental exposure. Neurotoxicology 2003, 24:449-462.
5. Branchi I., Capone F., Vitalone A., Madia F., Santucci D., Alleva E., et al. Early developmental exposure to BDE 99 or Aroclor 1254 affects neurobehavioural profile: interference from the administration route. Neurotoxicology 2005, 26:183-192.
6. Costa L.G., Giordano G. Developmental neurotoxicity of polybrominated diphenyl ether (PBDE) flame retardants. Neurotoxicology 2007, 28:1047-1067.
7. Das K.P., Barone S. Neuronal differentiation in PC12 cells is inhibited by chlorpyrifos and its metabolites: is acetylcholinesterase inhibition the site of action?. Toxicol. Appl. Pharmacol. 1999, 160:217-230.
8. Dingemans MML, van Den Berg M, Bergman Å, Westerink RHS. Calcium-related processes involved in the inhibition of depolarization-evoked calcium increase by hydroxylated PBDEs in PC12 cells. Toxicol Sci in press.
9. Dingemans M.M.L., Heusinkveld H.J., Bergman Å., van Den Berg M., Westerink R.H.S. Bromination pattern of hydroxylated metabolites of BDE-47 affects their potency to release calcium from intracellular stores in PC12 cells. Environ. Health Perspect. 2010, 118:519-525.
10. Dingemans M.M.L., van Den Berg M., Westerink R.H.S. Neurotoxicity of brominated flame retardants: (in)direct effects of parent and hydroxylated polybrominated diphenyl ethers on the (developing) nervous system. Environ. Health Perspect. 2011, 119:900-907.
11. Dishaw L.V., Powers C.M., Ryde I.T., Roberts S.C., Seidler F.J., Slotkin T.A., et al. Is the PentaBDE replacement, tris (1,3-dichloro-2-propyl) phosphate (TDCPP), a developmental neurotoxicant? Studies in PC12 cells. Toxicol. Appl. Pharmacol. 2011, 256:281-289.
12. Eriksson P., Viberg H., Jakobsson E., Orn U., Fredriksson A. A brominated flame retardant, 2,2',4,4',5-pentabromodiphenyl ether: uptake, retention, and induction of neurobehavioral alterations in mice during a critical phase of neonatal brain development. Toxicol. Sci. 2002, 67:98-103.
13. Fischer C., Fredriksson A., Eriksson P. Coexposure of neonatal mice to a flame retardant PBDE 99 (2,2',4,4',5-pentabromodiphenyl ether) and methyl mercury enhances developmental neurotoxic defects. Toxicol. Sci. 2008, 101:275-285.
14. Flaskos J., Nikolaidis E., Harris W., Sachana M., Hargreaves A.J. Effects of sub-lethal neurite outgrowth inhibitory concentrations of chlorpyrifos oxon on cytoskeletal proteins and acetylcholinesterase in differentiating N2a cells. Toxicol. Appl. Pharmacol. 2011, 256:330-336.
15. Fujita K., Lazarovici P., Guroff G. Regulation of the differentiation of PC12 pheochromocytoma cells. Environ. Health Perspect. 1989, 80:127-142.
16. A and α4β2 nicotinic acetylcholine receptors. Toxicol. Sci. 2010, 118:635-642.
17. Herbstman J.B., Sjodin A., Kurzon M., Lederman S.A., Jones R.S., Rauh V., et al. Prenatal exposure to PBDEs and neurodevelopment. Environ. Health Perspect. 2010, 118:712-719.
18. Howard A.S., Bucelli R., Jett D.A., Bruun D., Yang D.R. Chlorpyrifos exerts opposing effects on axonal and dendritic growth in primary neuronal cultures. Toxicol. Appl. Pharmacol. 2005, 207:112-124.
19. Huang S.C., Giordano G., Costa L.G. Comparative cytotoxicity and intracellular accumulation of five polybrominated diphenyl ether congeners in mouse cerebellar granule neurons. Toxicol. Sci. 2010, 114:124-132.
20. Jameson R.R., Seidler F.J., Qiao D., Slotkin T.A. Adverse neurodevelopmental effects of dexamethasone modeled in PC12 cells: identifying the critical stages and concentration thresholds for the targeting of cell acquisition, differentiation and viability. Neuropsychopharmacology 2006, 31:1647-1658.
21. Jameson R.R., Seidler F.J., Qiao D., Slotkin T.A. Chlorpyrifos affects phenotypic outcomes in a model of mammalian neurodevelopment: critical stages targeting differentiation in PC12 cells. Environ. Health Perspect. 2006, 114:667-672.
22. Lassiter T.L., MacKillop E.A., Ryde I.T., Seidler F.J., Slotkin T.A. Is fipronil safer than chlorpyrifos? Comparative developmental neurotoxicity modeled in PC12 cells. Brain Res. Bull. 2009, 78:313-322.
23. Norén K., Meironyté D. Certain organochlorine and organobromine contaminants in Swedish human milk in perspective of past 20-30years. Chemosphere 2000, 40:1111-1123.
24. Powers C.M., Wrench N., Ryde I.T., Smith A.M., Seidler F.J., Slotkin T.A. Silver impairs neurodevelopment: studies in PC12 cells. Environ. Health Perspect. 2010, 118:73-79.
25. Qiao D., Seidler F.J., Slotkin T.A. Developmental neurotoxicity of chlorpyrifos modeled in vitro: comparative effects of metabolites and other cholinesterase inhibitors on DNA synthesis in PC12 and C6 cells. Environ. Health Perspect. 2001, 109:909-913.
26. Qiao D., Seidler F.J., Violin J.D., Slotkin T.A. Nicotine is a developmental neurotoxicant and neuroprotectant: stage-selective inhibition of DNA synthesis coincident with shielding from effects of chlorpyrifos. Dev Brain Res 2003, 147:183-190.
27. Qiao D., Seidler F.J., Slotkin T.A. Oxidative mechanisms contributing to the developmental neurotoxicity of nicotine and chlorpyrifos. Toxicol. Appl. Pharmacol. 2005, 206:17-26.
28. Roy T.S., Sharma V., Seidler F.J., Slotkin T.A. Quantitative morphological assessment reveals neuronal and glial deficits in hippocampus after a brief subtoxic exposure to chlorpyrifos in neonatal rats. Dev Brain Res 2005, 155:71-80.
29. Roze E., Meijer L., Bakker A., Van Braeckel K.N.J.A., Sauer P.J.J., Bos A.F. Prenatal exposure to organohalogens, including brominated flame retardants, influences motor, cognitive, and behavioral performance at school age. Environ. Health Perspect. 2009, 117:1953-1958.
30. Schreiber T., Gassmann K., Götz C., Huubenthal U., Moors M., Krause G., et al. Polybrominated diphenylethers induce developmental neurotoxicity in a human in vitro model: evidence for endocrine disruption. Environ. Health Perspect. 2010, 118:572-578.
31. Slotkin T.A., Seidler F.J. Oxidative stress from diverse developmental neurotoxicants: antioxidants protect against lipid peroxidation without preventing cell loss. Neurotoxicol. Teratol. 2010, 32:124-131.
32. Slotkin T.A., MacKillop E.A., Ryde I.T., Seidler F.J. Ameliorating the developmental neurotoxicity of chlorpyrifos: a mechanisms-based approach in PC12 cells. Environ. Health Perspect. 2007, 115:1306-1313.
33. Slotkin T.A., MacKillop E.A., Ryde I.T., Tate C.A., Seidler F.J. Screening for developmental neurotoxicity using PC12 cells: comparisons of organophosphates with a carbamate, an organochlorine and divalent nickel. Environ. Health Perspect. 2007, 115:93-101.
34. Slotkin T.A., MacKillop E.A., Melnick R.L., Thayer K.A., Seidler F.J. Developmental neurotoxicity of perfluorinated chemicals modeled in vitro. Environ. Health Perspect. 2008, 116:716-722.
35. Song X., Violin J.D., Seidler F.J., Slotkin T.A. Modeling the developmental neurotoxicity of chlorpyrifos in vitro: macromolecule synthesis in PC12 cells. Toxicol. Appl. Pharmacol. 1998, 151:182-191.
36. Tagliaferri S., Caglieri A., Goldoni M., Pinelli S., Alinovi R., Poli D., et al. Low concentrations of the brominated flame retardants BDE-47 and BDE-99 induce synergistic oxidative stress-mediated neurotoxicity in human neuroblastoma cells. Toxicol in Vitro 2010, 24:116-122.
37. Teng K.K., Greene L.A. Cultured PC12 cells: a model for neuronal function and differentiation. Cell Biology: A Laboratory Handbook 1994, 218-224. Academic Press, San Diego. J.E. Celis (Ed.).
38. U.S. Environmental Protection Agency, 2006. Opportunities to Improve Data Quality and Children's Health Through the Food Quality Protection Act. Report no. 2006-P-00009. Vol., ed. eds.
39. Viberg H., Eriksson P. Differences in neonatal neurotoxicity of brominated flame retardants, PBDE 99 and TBBPA, in mice. Toxicology 2011, 289:59-65.
40. Viberg H., Fredriksson A., Eriksson P. Deranged spontaneous behaviour and decrease in cholinergic muscarinic receptors in hippocampus in the adult rat, after neonatal exposure to the brominated flame-retardant, 2,2',4,4',5-pentabromodiphenyl ether (PBDE99). Environ. Toxicol. Pharmacol. 2005, 20:283-288.
41. Winick M., Noble A. Quantitative changes in DNA, RNA and protein during prenatal and postnatal growth in the rat. Dev. Biol. 1965, 12:451-466.
42. Yang D., Howard A., Bruun D., Ajua-Alemanj M., Pickart C., Lein P.J. Chlorpyrifos and chlorpyrifos-oxon inhibit axonal growth by interfering with the morphogenic activity of acetylcholinesterase. Toxicol. Appl. Pharmacol. 2008, 228:32-41.