Development of a recombinant N-Gp5c fusion protein-based ELISA for detection of antibodies to porcine reproductive and respiratory syndrome virus

Journal of Virological Methods

Volumn 189, Issue 1, 2013, Pages 213-220

  Chen, Caiwei ^a,b   Fan, Wenhui ^a,c   Jia, Xiaojuan ^a   Li, Jing ^a   Bi, Yuhai ^a   Liu, Wenjun ^a,b  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c GUANGXI UNIVERSITY   (China)

Author keywords

Antibody detection; Gp5; Indirect ELISA; Nucleocapsid protein; PRRSV

Indexed keywords

HYBRID PROTEIN; NUCLEOCAPSID PROTEIN; RECOMBINANT FUSION PROTEIN N GP5C; UNCLASSIFIED DRUG;

ANTIBODY DETECTION; ARTERIVIRUS; ARTERIVIRUS INFECTION; ARTICLE; CLINICAL EFFECTIVENESS; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE SEQUENCE; IMMUNOFLUORESCENCE TEST; INDIRECT ENZYME LINKED IMMUNOSORBENT ASSAY; MEASUREMENT REPEATABILITY; MOLECULAR CLONING; N GP5C GENE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; VALIDATION PROCESS;

ANIMALS; ANTIBODIES, VIRAL; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; NUCLEOCAPSID PROTEINS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SWINE; SWINE DISEASES; VIRAL ENVELOPE PROTEINS;

ESCHERICHIA COLI; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE; SUS;

EID: 84875537131     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.02.003     Document Type: Article

References (35)

1. Albina E., Leforban Y., Baron T., Plana Duran J.P., Vannier P. An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus. Ann. Rech. Vet. 1992, 23:167-176.
2. Albina E., Madec F., Cariolet R., Torrison J. Immune response and persistence of the porcine reproductive and respiratory syndrome virus in infected pigs and farm units. Vet. Rec. 1994, 134:567-573.
3. Benfield D.A., Nelson E., Collins J.E., Harris L., Goyal S.M., Robison D., Christianson W.T., Morrison R.B., Gorcyca D., Chladek D. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J. Vet. Diagn. Invest. 1992, 4:127-133.
4. Chen Y., Tian H., He J.H., Wu J.Y., Shang Y.J., Liu X.T. Indirect ELISA with recombinant GP5 for detecting antibodies to porcine reproductive and respiratory syndrome virus. Virol. Sin. 2011, 26:61-66.
5. Cho H.J., Deregt D., Joo H.S. An ELISA for porcine reproductive and respiratory syndrome: production of antigen of high quality. Can. J. Vet. Res. 1996, 60:89-93.
6. Cho S.H., Freese W.R., Yoon I.J., Trigo A.V., Joo H.S. Seroprevalence of indirect fluorescent antibody to porcine reproductive and respiratory syndrome virus in selected swine herds. J. Vet. Diagn. Invest. 1993, 5:259-260.
7. Crowther J.R. The ELISA Guidebook 2000, Humana, New York. 2nd ed.
8. Dea S., Wilson L., Therrien D., Cornaglia E. Competitive ELISA for detection of antibodies to porcine reproductive and respiratory syndrome virus using recombinant E. coli-expressed nucleocapsid protein as antigen. J. Virol. Methods 2000, 87:109-122.
9. Denac H., Moser C., Tratschin J.D., Hofmann M.A. An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen. J. Virol. Methods 1997, 65:169-181.
10. Ferrin N.H., Fang Y., Johnson C.R., Murtaugh M.P., Polson D.D., Torremorell M., Gramer M.L., Nelson E.A. Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 2004, 11:503-514.
11. Gonin P., Pirzadeh B., Gagnon C.A., Dea S. Seroneutralization of porcine reproductive and respiratory syndrome virus correlates with antibody response to the GP5 major envelope glycoprotein. J. Vet. Diagn. Invest. 1999, 11:20-26.
12. Houben S., Callebaut P., Pensaert M.B. Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs. J. Virol. Methods 1995, 51:125-128.
13. Jacobson R.H. Validation of serological assays for diagnosis of infectious diseases. Rev. Sci. Tech. 1998, 17:469-526.
14. Jiang Y.B., Fang L.R., Xiao S.B., Xie T.T., Chen H.C. Expression of GP5-M fusion protein of porcine reproductive and respiratory syndrone virus (PRRSV) and establishment of ELISA diagnose based on the recombinant fusion protein. Sheng Wu Gong Cheng Xue Bao 2005, 21:259-264.
15. Johnson C.R., Griggs T.F., Gnanandarajah J., Murtaugh M.P. Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses. J. Gen. Virol. 2011, 92:1107-1116.
16. Morrison R.B., Collins J.E., Harris L., Christianson W.T., Benfield D.A., Chladek D.W., Gorcyca D.E., Joo H.S. Serologic evidence incriminating a recently isolated virus (ATCC VR-2332) as the cause of swine infertility and respiratory syndrome (SIRS). J. Vet. Diagn. Invest. 1992, 4:186-188.
17. Nelson E.A., Christopher-Hennings J., Benfield D.A. Structural proteins of porcine reproductive and respiratory syndrome virus (PRRSV). Adv. Exp. Med. Biol. 1995, 380:321-323.
18. Nelson E.A., Christopher-Hennings J., Drew T., Wensvoort G., Collins J.E., Benfield D.A. Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J. Clin. Microbiol. 1993, 31:3184-3189.
19. Oleksiewicz M.B., Botner A., Normann P. Semen from boars infected with porcine reproductive and respiratory syndrome virus (PRRSV) contains antibodies against structural as well as nonstructural viral proteins. Vet. Microbiol. 2001, 81:109-125.
20. Oleksiewicz M.B., Botner A., Toft P., Normann P., Storgaard T. Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. J. Virol. 2001, 75:3277-3290.
21. Ostrowski M., Galeota J.A., Jar A.M., Platt K.B., Osorio F.A., Lopez O.J. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J. Virol. 2002, 76:4241-4250.
22. Plagemann P.G. Neutralizing antibody formation in swine infected with seven strains of porcine reproductive and respiratory syndrome virus as measured by indirect ELISA with peptides containing the GP5 neutralization epitope. Viral Immunol. 2006, 19:285-293.
23. Pyo H., Seo J., Suh G., Kim K., Lee J., Kim T. Serodiagnosis of porcine reproductive and respiratory syndrome virus infection with the use of glycoprotein 5 antigens. Can. J. Vet. Res. 2010, 74:223-227.
24. Seuberlich T., Tratschin J.D., Thur B., Hofmann M.A. Nucleocapsid protein-based enzyme-linked immunosorbent assay for detection and differentiation of antibodies against European and North American porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 2002, 9:1183-1191.
25. Snijder E.J., Firth A.E., Zevenhoven-Dobbe J.C., Wills N.M., Go Y.Y., Balasuriya U.B.R., Atkins J.F., Posthuma C.C. Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production. J. Gen. Virol. 2011, 92:1097-1106.
26. Stadejek T., Stankevicius A., Storgaard T., Oleksiewicz M.B., Belak S., Drew T.W., Pejsak Z. Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses. J. Gen. Virol. 2002, 83:1861-1873.
27. Takikawa N., Kobayashi S., Ide S., Yamane Y., Tanaka Y., Yamagishi H. Detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus in swine sera by enzyme-linked immunosorbent assay. J. Vet. Med. Sci. 1996, 58:355-357.
28. Tian K., Yu X., Zhao T., Feng Y., Cao Z., Wang C., Hu Y., Chen X., Hu D., Tian X., Liu D., Zhang S., Deng X., Ding Y., Yang L., Zhang Y., Xiao H., Qiao M., Wang B., Hou L., Wang X., Yang X., Kang L., Sun M., Jin P., Wang S., Kitamura Y., Yan J., Gao G.F. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE 2007, 2:e526.
29. Wensvoort G., Terpstra C., Pol J.M., ter Laak E.A., Bloemraad M., de Kluyver E.P., Kragten C., van Buiten L., den Besten A., Wagenaar F., et al. Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet. Q. 1991, 13:121-130.
30. Witte S.B., Chard-Bergstrom C., Loughin T.A., Kapil S. Development of a recombinant nucleoprotein-based enzyme-linked immunosorbent assay for quantification of antibodies against porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 2000, 7:700-702.
31. Yoon I.J., Joo H.S., Christianson W.T., Kim H.S., Collins J.E., Carlson J.H., Dee S.A. Isolation of a cytopathic virus from weak pigs on farms with a history of swine infertility and respiratory syndrome. J. Vet. Diagn. Invest. 1992, 4:139-143.
32. Yoon I.J., Joo H.S., Christianson W.T., Kim H.S., Collins J.E., Morrison R.B., Dial G.D. An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J. Vet. Diagn. Invest. 1992, 4:144-147.
33. Yoon I.J., Joo H.S., Goyal S.M., Molitor T.W. A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera. J. Vet. Diagn. Invest. 1994, 6:289-292.
34. Yoon K.J., Zimmerman J.J., Swenson S.L., McGinley M.J., Eernisse K.A., Brevik A., Rhinehart L.L., Frey M.L., Hill H.T., Platt K.B. Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection. J. Vet. Diagn. Invest. 1995, 7:305-312.
35. Zhou Y.J., Yu H., Tian Z.J., Liu J.X., An T.Q., Peng J.M., Li G.X., Jiang Y.F., Cai X.H., Xue Q., Wang M., Wang Y.F., Tong G.Z. Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Vet. Microbiol. 2009, 138:1-10.