Detecting gene expression in buccal mucosa in subjects with asthma versus subjects without asthma

Pediatric Allergy and Immunology

Volumn 24, Issue 2, 2013, Pages 138-143

  Vyhlidal, Carrie A ^a   Riffel, Amanda K ^a   Dai, Hongying ^a   Rosenwasser, Lanny J ^a   Jones, Bridgette L ^a  

^a CHILDREN S MERCY HOSPITAL   (United States)

Author keywords

Asthma; Biomarkers; Buccal mucosa; Corticosteroids; Gene expression

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HISTAMINE H1 RECEPTOR; INTERCELLULAR ADHESION MOLECULE 1; INTERLEUKIN 8; MESSENGER RNA; MONOCYTE CHEMOTACTIC PROTEIN 1; MUCIN 5B; RNA; SERINE PROTEINASE INHIBITOR; TRANSCRIPTION FACTOR RELA; TRANSCRIPTION FACTOR RUNX2;

ADOLESCENT; ADULT; ARTICLE; ASTHMA; CHEEK MUCOSA; CHILD; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; GENETIC ASSOCIATION; HUMAN; MAJOR CLINICAL STUDY; MALE; PRESCHOOL CHILD; PRIORITY JOURNAL; RESPIRATORY EPITHELIUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SCHOOL CHILD;

ADOLESCENT; ADULT; ASTHMA; CASE-CONTROL STUDIES; CHILD; CHILD, PRESCHOOL; FEASIBILITY STUDIES; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC MARKERS; GENETIC PREDISPOSITION TO DISEASE; HUMANS; MALE; MIDDLE AGED; MOUTH MUCOSA; PHENOTYPE; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PROGNOSIS; REPRODUCIBILITY OF RESULTS; RNA STABILITY; RNA, MESSENGER; YOUNG ADULT;

EID: 84875521015     PISSN: 09056157     EISSN: 13993038     Source Type: Journal    
DOI: 10.1111/pai.12042     Document Type: Article

References (25)

1. Moorman JE, Zahran H, Truman BI, Molla MT. Current asthma prevalence-united states, 2006-2008. MMWR 2011: 60: 84-6.
2. Garcia-Marcos L, Brand PL. The utility of sputum eosinophils and exhaled nitric oxide for monitoring asthma control with special attention to childhood asthma. Allergol Immunopathol 2010: 38: 41-6.
3. Crane J, Lampshire P, Wickens K, et al. Asthma, atopy and exhaled nitric oxide in a cohort of 6-yr-old New Zealand children. Pediatr Allergy Immunol 2012: 23: 59-64.
4. Kulkarni N, Ragazzo V, Costella S, et al. Eosinophilic airway inflammation is increased in children with asthma and food allergies. Pediatr Allergy Immunol 2012: 23: 28-33.
5. Linkosalo L, Lehtimaki L, Holm K, Kaila M, Moilanen E. Relation of bronchial and alveolar nitric oxide to exercise-induced bronchoconstriction in atopic children and adolescents. Pediatr Allergy Immunol 2012: 23: 360-6.
6. Brutsche MH, Joos L, Carlen Brutsche IE, et al. Array-based diagnostic gene-expression score for atopy and asthma. J Allergy Clin Immunol 2002: 109: 271-3.
7. Woodruff PG, Boushey HA, Dolganov GM, et al. Genome-wide profiling identifies epithelial cell genes associated with asthma and with treatment response to corticosteroids. Proc Natl Acad Sci USA 2007: 104: 15858-63.
8. Yuyama N, Davies DE, Akaiwa M, et al. Analysis of novel disease-related genes in bronchial asthma. Cytokine 2002: 19: 287-96.
9. Hakonarson H, Bjornsdottir US, Halapi E, et al. Profiling of genes expressed in peripheral blood mononuclear cells predicts glucocorticoid sensitivity in asthma patients. Proc Natl Acad Sci USA 2005: 102: 14789-94.
10. Hew M, Bhavsar P, Torrego A, et al. Relative corticosteroid insensitivity of peripheral blood mononuclear cells in severe asthma. Am J Respir Crit Care Med 2006: 174: 134-41.
11. Guajardo JR, Schleifer KW, Daines MO, et al. Altered gene expression profiles in nasal respiratory epithelium reflect stable versus acute childhood asthma. J Allergy Clin Immunol 2005: 115: 243-51.
12. Kumar SV, Jain R, Mokhiber K, Venezia A, Sheehan A, Spivack SD. Exfoliated buccal and microdissected lung cell expression of antioxidant enzymes. Cancer Detect Prev 2005: 29: 552-61.
13. Michalczyk A, Varigos G, Smith L, Ackland ML. Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis. Biotechniques 2004: 37: 262-4, 66-9.
14. Spira A, Beane J, Schembri F, et al. Noninvasive method for obtaining RNA from buccal mucosa epithelial cells for gene expression profiling. Biotechniques 2004: 36: 484-7.
15. Spivack SD, Hurteau GJ, Jain R, et al. Gene-environment interaction signatures by quantitative mRNA profiling in exfoliated buccal mucosal cells. Cancer Res 2004: 64: 6805-13.
16. Sridhar S, Schembri F, Zeskind J, et al. Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium. BMC genomics 2008: 9: 259.
17. NHLBI. Expert panel report 3 (EPR-3): guidelines for the diagnosis and management of asthma-summary report 2007. J Allergy Clin Immunol 2007: 120: S94-138.
18. Fleige S, Pfaffl MW. RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 2006: 27: 126-39.
19. Antonov J, Goldstein DR, Oberli A, et al. Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization. Lab Invest 2005: 85: 1040-50.
20. Ribeiro-Silva A, Zhang H, Jeffrey SS. RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies. BMC Mol Biol 2007: 8: 118.
21. Selivanova PA, Kulikov ES, Kozina OV, Gereng EA, Freidin MB, Ogorodova LM. Morphological and molecular characteristics of "difficult" asthma. J Asthma 2010: 47: 269-75.
22. Van Ganse E, Kaufman L, Derde MP, Yernault JC, Delaunois L, Vincken W. Effects of antihistamines in adult asthma: a meta-analysis of clinical trials. Eur Respir J 1997: 10: 2216-24.
23. Heinzmann A, Ahlert I, Kurz T, Berner R, Deichmann KA. Association study suggests opposite effects of polymorphisms within IL8 on bronchial asthma and respiratory syncytial virus bronchiolitis. J Allergy Clin Immunol 2004: 114: 671-6.
24. Cheong HS, Shin HD, Lee SO, et al. Polymorphisms in interleukin 8 and its receptors (IL8, IL8RA and IL8RB) and association of common IL8 receptor variants with peripheral blood eosinophil counts. J Hum Genet 2006: 51: 781-7.
25. Hillian AD, Londono D, Dunn JM, et al. Modulation of cystic fibrosis lung disease by variants in interleukin-8. Genes Immun 2008: 9: 501-8.