High frequency of virulence factor genes tdh, trh, and tlh in Vibrio parahaemolyticus strains isolated from a pristine estuary

Applied and Environmental Microbiology

Volumn 79, Issue 7, 2013, Pages 2247-2252

  Gutierrez West, Casandra K ^a   Klein, Savannah L ^a   Lovell, harles R ^a  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHROPOGENIC INFLUENCE; ESTUARINE ECOSYSTEMS; HUMAN PATHOGENS; MOLECULAR MARKER; THERMOSTABLE DIRECT; V. PARAHAEMOLYTICUS; VIBRIO PARAHAEMOLYTICUS; VIRULENCE FACTORS;

BLOOD; POLYMERASE CHAIN REACTION;

GENE ENCODING;

BACTERIAL DNA; HEMOLYSIN; VIRULENCE FACTOR;

ANTHROPOGENIC EFFECT; BACTERIUM; ESTUARINE ECOSYSTEM; GENE EXPRESSION; GENETIC ANALYSIS; PRISTINE ENVIRONMENT; PROTEIN; PUBLIC HEALTH; VIRULENCE;

ARTICLE; DNA SEQUENCE; ESTUARY; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; VIBRIO PARAHAEMOLYTICUS;

DNA, BACTERIAL; ESTUARIES; HEMOLYSIN PROTEINS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; VIBRIO PARAHAEMOLYTICUS; VIRULENCE FACTORS; WATER MICROBIOLOGY;

NORTH INLET; SOUTH CAROLINA; UNITED STATES;

VIBRIO PARAHAEMOLYTICUS;

EID: 84875521011     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03792-12     Document Type: Article

References (36)

1. Food and Drug Administration. 2005. Vibrio parahaemolyticus risk assessment: quantitative risk assessment on the public health impact of pathogenic Vibrio parahaemolyticus in raw oysters. Food and Drug Administration, Silver Spring, MD. http://www.fda.gov/Food/ScienceResearch/ResearchAreas/Risk AssessmentSafetyAssessment/ucm050421.htm.
2. Broberg CA, Calder TJ, Orth K. 2011. Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes Infect. 13:992-1001.
3. Shirai H, Ito H, Hirayama T, Nakamoto Y, Nakabayashi N, Kumagai K, Takeda Y, Nishibuchi M. 1990. Molecular, epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infect. Immun. 58:3568-3573.
4. Honda T, Iida T. 1993. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct hemoloysin and related hemolysin. Rev. Med. Microbiol. 4:106-113.
5. Bej AK, Patterson DP, Brasher CW, Vickery MCL, Jones DD, Kaysner CA. 1999. Detection of total and hemolysin producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tlh, tdh and trh. J. Microbiol. Methods 36:215-225.
6. Ohnishi K, Nakahira K, Unzai S, Mayanagi K, Hashimoto H, Shiraki K, Honda T, Yanagihara I. 2011. Relationship between heat-induced fibrillogenicity and hemolytic activity of thermostable direct hemolysin and a related hemolysin of Vibrio parahaemolyticus. FEMS Microbiol. Lett. 318:10-17.
7. Yanagihara I, Nakahira K, Yamane T, Kaieda S, Mayanagi K, Hamada D, Fukui T, Ohnishi K, Kajiyama S, Shimizu T, Sato M, Ikegami T, Ikeguchi M, Honda T, Hashimoto H. 2010. Structure and functional characterization of Vibrio parahaemolyticus thermostable direct hemolysin. J. Biol. Chem. 285:16267-16274.
8. Raimondi F, Kao JPY, Fiorentini C, Fabbri A, Donelli G, Gasparini N. 2000. Enterotoxicity and cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin in vitro systems. Infect. Immun. 68:3180-3185.
9. DePaola A, Kaysner CA. 2004. Vibrio. Bacteriological analytical manual online. U.S. Food and Drug Administration, Washington, DC. http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070830.htm.
10. Zhang HZ, Austin B. 2005. Haemolysins in Vibrio species. J. Appl. Microbiol. 98:1011-1019.
11. Gotoh K, Kodama T, Hiyoshi H, Izutsu K, Park KS, Dryselius R, Akeda Y, Honda T, Iida T. 2010. Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants. PLoS One 5:e13365. doi:10.1371/journal.pone.0013365.
12. DePaola A, Nordstrom JL, Bowers JC, Wells JG, Cook DW. 2003. Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters. Appl. Environ. Microbiol. 69:1521-1526.
13. Kaneko T, Colwell RR. 1975. Adsorption of V. parahaemolyticus onto chitin and copepods. Appl. Microbiol. 29:269-274.
14. Kaneko T, Colwell RR. 1973. Ecology of Vibrio parahaemolyticus in Chesapeake Bay. J. Bacteriol. 113:24-32.
15. McCarter L. 1999. The multiple identities of Vibrio parahaemolyticus. J. Mol. Microbiol. Biotechnol. 1:51-57.
16. Gamble MD, Lovell CR. 2011. Infaunal burrows are enrichment zones for Vibrio parahaemolyticus. Appl. Environ. Microbiol. 77:3703-3714.
17. Nishibuchi M, Kaper JB. 1995. Minireview. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect. Immun. 63:2093-2099.
18. Vieira RH, Costa RA, Menezes FG, Silvia GC, Theophilo GN, Rodrigues DP, Maggiono R. 2011. Kanagawa-negative, tdh- and trh-positive Vibrio parahaemolyticus isolated from fresh oysters marketed in Fortaleza, Brazil. Curr. Microbiol. 63:126-130.
19. Baker-Austin C, McArthur JV, Tuckfield RC, Najarro M, Lindell AH, Gooch J, Stepanauskas R. 2008. Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carolina, USA. J. Food Prot. 71:2552-2558.
20. Velazquez-Roman J, Leon-Sicairos N, Flores-Villasenor H, Villafauna- Rauda S, Canizalez-Roman A. 2012. Association of pandemic Vibrio parahaemolyticus O3:K6 present in the coastal environment of northwest Mexico with cases of recurrent diarrhea between 2004 and 2010. Appl. Environ. Microbiol. 78:1794-1803.
21. Paranjpye R, Hamel OS, Stojanovski A, Liermann M. 2012. Genetic diversity of clinical and environmental Vibrio parahaemolyticus strains from the Pacific Northwest. Appl. Environ. Microbiol. 78:8631-8638.
22. Dame RF, et al. 2000. Estuaries of the South Atlantic coast of North America: their geographical signatures. Estuaries 23:793-819.
23. Buzzelli C, Akman O, Buck T, Koepfler E, Morris J, Lewitus A. 2004. Relationships among water quality parameters from the North Inlet- Winyah Bay National Estuarine Research Reserve, South Carolina. J. Coast. Res. 45:59-74.
24. Dame RF. 1989. The importance of Spartina alterniflora to Atlantic coast estuaries. Crit. Rev. Aquat. Sci. 1:639-660.
25. Gamble MD, Bagwell CE, LaRocque J, Bergholz PW, Lovell CR. 2010. Seasonal variability of diazotroph assemblages associated with the rhizosphere of the salt marsh cordgrass, Spartina alterniflora. Microb. Ecol. 59:253-265.
26. Yoch DC, Whiting GJ. 1986. Evidence for NH4+switch-off regulation of nitrogenase activity by bacteria in salt marsh sediments and roots of the grass Spartina alterniflora. Appl. Environ. Microbiol. 51:143-149.
27. Piceno YM, Noble PA, Lovell CR. 1999. Spatial and temporal assessment of diazotroph assemblage composition in vegetated salt marsh sediments using denaturing gradient gel electrophoresis (DGGE) analysis. Microb. Ecol. 38:157-167.
28. Bagwell CE, Piceno YM, Ashburne-Lucas A, Lovell CR. 1998. Physiological diversity of the rhizosphere diazotroph assemblages of selected salt marsh grasses. Appl. Environ. Microbiol. 64:4276-4282.
29. Thompson CC, Thompson FL, Vandemeulebroecke K, Hoste B, Dawyndt P, Swings J. 2004. Use of recA as an alternative phylogenetic marker in the family Vibrionaceae. Int. J. Syst. Evol. Microbiol. 54:919-924.
30. Hazen T, Pan L, Gu JD, Sobecky P. 2010. The contribution of mobile genetic elements to the evolution and ecology of Vibrios. FEMS Microbiol. Ecol. 74:485-499.
31. Thompson FL, Gevers D, Thompson CC, Dawnydt P, Naser S, Hoste B, Munn CB, Swings J. 2005. Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl. Environ. Microbiol. 71:5107-5115.
32. Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599.
33. Kishishita M, Matsuoka N, Kumagai K, Yamasaki S, Takeda Y, Nishibuchi M. 1992. Sequence variation in the thermostable direct hemolysinrelated hemolysin (trh) gene of Vibrio parahaemolyticus. Appl. Environ. Microbiol. 58:2449-2457.
34. Yeung PSM, Hayes M, DePaola A, Kaysner CA, Kornstein L, Boor K. 2002. Comparative phenotypic, molecular and virulence characterization of Vibrio parahaemolyticus O3:K6 isolates. Appl. Environ. Microbiol. 68:2901-2909.
35. Brown MM, Friez MJ, Lovell CR. 2003. Expression of nifH genes by diazotrophic bacteria in the rhizosphere of short form Spartina alterniflora. FEMS Microbiol. Ecol. 43:411-417.
36. Raymond PA, Bauer JE. 2000. Bacterial consumption of DOC during transport through a temperate estuary. Aquat. Microb. Ecol. 22:1-12.