Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle

Journal of Visualized Experiments

Volumn , Issue 73, 2013, Pages

  Pasut, Alessandra ^a,b   Jones, Andrew E ^a,b   Rudnicki, Michael A ^a,b  

^a OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

Author keywords

Anatomy; Animal model; Bioengineering; Biomedical engineering; Cell culture; Cellular biology; Duchenne; Hindlimb; Immunostaining; Isolation and culture of isolated myofibers; Issue 73; Medicine; Molecular biology; Mouse; Muscle regeneration; Muscles; Muscular dystrophy; Myoblasts; Myofiber; Pax7; Physiology; Satellite cells; Skeletal; Skeletal muscle; Stem cell biology; Stem cells; Tissue culture techniques; Tissue engineering

Indexed keywords

ANIMAL; ARTICLE; CATTLE; CELL SEPARATION; CHICK EMBRYO; CULTURE TECHNIQUE; CYTOLOGY; HORSE; METHODOLOGY; MOUSE; SKELETAL MUSCLE; SKELETAL MUSCLE SATELLITE CELL;

ANIMALS; CATTLE; CELL CULTURE TECHNIQUES; CELL SEPARATION; CHICK EMBRYO; HORSES; MICE; MUSCLE FIBERS, SKELETAL; MUSCLE, SKELETAL; SATELLITE CELLS, SKELETAL MUSCLE;

EID: 84875436996     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50074     Document Type: Article

References (18)

1. Bischoff, R. Proliferation of muscle satellite cells on intact myofibers in culture. Dev. Biol. 115 (1), 129-139 (1986).
2. Rosenblatt, J.D., Lunt, A.I., Parry, D.J., & Partridge, T.A. Culturing satellite cells from living single muscle fiber explants. In Vitro Cell Dev. Biol. Anim. 31 (10), 773-9 (1995).
3. Anderson, J.E., Wozniak, A.C., & Misunoya W. Single muscle fiber isolation and culture for cellular molecular, pharmacological, and evolutionary studies. Methods Mol. Biol. 798, 85-102, Humana Press, New York, NY, 10013, USA, (2012).
4. Shefer, G. & Yablonka-Reuveni, Z. Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells. Methods Mol. Biol. 290, 281-304, Human Press Inc, Totowa, NJ, (2005).
5. White, R.B., Biérinx, A.S., Gnocchi, V.F., & Zammit, P.S. Dynamics of muscle fibre growth during postnatal mouse development. BMC Developmental Biology. 10 (21), 1-11 (2010).
6. Siegel, A.L., Kuhlmann, P.K., & Cornelison, D.D. Muscle satellite cell proliferation and association: new insights from myofiber time-lapse imaging. Skeletal Muscle. 2 (1), 1-7 (2011).
7. Pasut, A., Oleynik, P., & Rudnicki, M.A. Isolation of muscle stem cells by fluorescence activated cell sorting cytometry. Methods Mol. Biol. 798, 53-64, Humana Press, New York, NY, 10013, USA, (2012).
8. Engler, A.D., Griffin, M.A., Sen, S., Bonnemann, C.G., Sweeney, H.L., & Discher, D.E. Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments. J. Cell. Biol. 166 (4), 877-87 (2004).
9. Boonen, K.J. & Post, M.J. The muscle stem cell niche: regulation of satellite cells during regeneration. Tissue Eng. Part B Rev. 14 (4), 419-31 (2008).
10. Wozniak, A.C. & Anderson, J.E. Single-fiber isolation and maintenance of satellite cell quiescence. Biochem. Cell Biol. 83 (5), 674-6 (2005).
11. Verma, M. & Asukura, A. Efficient Single Muscle Fiber Isolation from Alcohol-Fixed Adult Muscle following β-Galactosidase Staining for Satellite Cell Detection. J. Histochem. Cytochem. 59 (1), 60-7 (2001).
12. Wosniak, A.C., Pilipowics, O., et al. C-Met expression and mechanical activation of satellite cells on cultured muscle fibers. J. Histochem. Cytochem. 51 (11), 1437-45 (2003).
13. Huard, J., Feero, W.G., Watkin, S.C., Hoffman, E.P., Rosenblatt, J.D., & Glorioso, J.C. The basal lamina is a physical barrier to herpes simplex virus-mediated gene delivery to mature muscle fibers. J. Virol. 70 (11), 8117-23 (1996).
14. Feero, W.G., Rosenblatt, J.D., et al. Viral gene delivery to skeletal muscle: insights on maturation-dependent loss of fiber infectivity for adenovirus and herpes simplex type 1 viral vectors. Hum. Gene Ther. 8 (4), 371-80 (1997).
15. Kuang, S., Kuroda, K., Le grand, F., & Rudnicki, M.A. Asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell. 129 (5), 999-1010 (2007).
16. Siegel, A.L, Atchison, K., Fisher, K.E., Davis, G.E., & Cornelison, DD. 3D timelapse analysis of muscle satellite cell motility. Stem Cells. 27 (10), 2527-38 (2009).
17. Seale, P., Sabourin, L.A., Girgis-Gabardo, A., Mansouri, A., Gruss, P., & Rudnicki, M.A. Pax7 is required for the specification of myogenic satellite cells. Cell. 102 (6), 777-86 (2000).
18. Zammit, P., Golding, J.P., Nagata, Y., Hudon, V., Partridge, T.A., & Beauchamp J.R. Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? J. Cell Biol. 166 (3), 347-57 (2004)