Ontogeny and molecular phylogeny of a new marine ciliate genus, Heterokeronopsis g. n. (Protozoa, Ciliophora, Hypotricha), with description of a new species

European Journal of Protistology

Volumn 49, Issue 2, 2013, Pages 298-311

  Pan, Ying ^a   Li, Jiamei ^a   Li, Lifang ^b   Hu, Xiaozhong ^a,c   Al Rasheid, Khaled A S ^d   Warren, Alan ^c  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b SHANDONG UNIVERSITY   (China)

^c DEPARTMENT OF LIFE SCIENCES   (United Kingdom)

^d KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Ciliophora; Heterokeronopsis; Hypotricha; New genus; Ontogeny; Phylogeny

Indexed keywords

PROTOZOAL DNA; PROTOZOAL RNA; RIBOSOME DNA; RNA 18S;

ARTICLE; CELL ORGANELLE; CHEMISTRY; CHINA; CILIATE; CLASSIFICATION; CLUSTER ANALYSIS; CYTOLOGY; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; RNA GENE; ULTRASTRUCTURE; WETLAND;

CHINA; CILIOPHORA; CLUSTER ANALYSIS; DNA, PROTOZOAN; DNA, RIBOSOMAL; ENVIRONMENTAL MICROBIOLOGY; GENES, RRNA; MOLECULAR SEQUENCE DATA; ORGANELLES; PHYLOGENY; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SEQUENCE ANALYSIS, DNA; WETLANDS;

BICORONA; CILIOPHORA; FASCIOLA; HYPOTRICHIA; PROTOZOA; PSEUDOKERONOPSIDAE;

EID: 84875381985     PISSN: 09324739     EISSN: 16180429     Source Type: Journal    
DOI: 10.1016/j.ejop.2012.08.008     Document Type: Article

References (33)

1. Berger H. Uroleptopsis Kahl, 1932 (Ciliophora, Hypotricha): morphology and cell division of type species, redefinition, and phylogenetic relationships. Acta Protozool. 2004, 43:99-121.
2. Berger H. Monograph of the Urostyloidea (Ciliophora, Hypotricha). Monograph. Biol. 2006, 85:1-1303.
3. Chen X., Li Z., Hu X., Kusuoka Y. Morphology, morphogenesis and gene sequence of a freshwater ciliate, Pseudourostyla cristata (Ciliophora, Urostylida) from the ancient Lake Biwa, Japan. Eur. J. Protistol. 2010, 46:43-60.
4. Chen X., Clamp J., Song W. Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis. Zool. Scr. 2011, 40:659-671.
5. Chen X., Dong J., Lin X. Morphology and phylogeny of a new urostylid ciliate, Monocoronella carnea n. gen., n. sp. (Ciliophora, Hypotrichia) from Daya Bay, southern China. J. Eukaryot. Microbiol. 2011, 58:497-503.
6. Guindon S., Gascuel O. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 2003, 52:696-704.
7. Hu X., Warren A., Song W. Observations on the morphology and morphogenesis of a new hypotrich ciliate (Ciliophora, Hypotrichida) from China. J. Nat. Hist. 2004, 38:1059-1069.
8. Hu X., Warren A., Suzuki T. Morphology and morphogenesis of two marine ciliates, Pseudokeronopsis pararubra sp. n. and Amphisiella annulata from China and Japan (Protozoa: Ciliophora). Acta Protozool. 2004, 43:351-368.
9. Jiang J., Zhang Q., Warren A., Al-Rasheid K.A.S., Song W. Morphology and SSU rRNA gene-based phylogeny of two marine Euplotes species, E. orientalis spec. nov. and E. raikovi Agamaliev, 1966 (Ciliophora, Euplotida). Eur. J. Protistol. 2010, 46:121-132.
10. Kumar S., Kamra K., Sapra G.R. Ciliates of the Silent Valley National Park. India: urostyloid hypotrichs of the region with a note on the habitat. Acta Protozool. 2010, 49:339-364.
11. Li L., Zhang Q., Hu X., Warren A., Al-Rasheid K.A.S., Al-Khedheiry A.A., Jiang J., Song W. A redescription of the marine hypotrichous ciliate. Nothoholosticha fasciola (Kahl, 1932) nov. gen., nov. comb. (Ciliophora: Urostylida) with brief notes on its cellular reorganization and SS rRNA gene sequence. Eur. J. Protistol. 2009, 45:237-248.
12. Li L., Khan S.N., Ji D., Shin M.K., Berger H. Morphology and small subunit (SSU) rRNA gene sequence of the new brackish water ciliate Neobakuella flava n. g., n. sp. (Ciliophora, Spirotricha, Bakuellidae) and SSU rRNA gene sequences of six additional hypotrichs from Korea. J. Eukaryot. Microbiol. 2011, 58:339-351.
13. Liu W., Li J., Gao S., Shao C., Gong J., Lin X., Liu H., Song W. Morphological studies and molecular data on a new marine ciliate. Apokeronopsis sinica n. sp. (Ciliophora: Urostylida), from the South China Sea. Zootaxa 2009, 2005:57-66.
14. Liu W., Shao C., Gong J., Li J., Lin X., Song W. Morphology, morphogenesis and molecular phylogeny of a new marine urostylid ciliate (Ciliophora, Stichotrichia) from the South China Sea, and an overview of the convergent evolution of midventral complex within the Spirotrichea. Zool. J. Linn. Soc. 2010, 158:697-710.
15. Lynn D.H. The Ciliated Protozoa: Characterization, Classification and Guide to the Literature 2008, Springer Press, Dordrecht. 3rd ed.
16. Medlin L., Elwood H.L., Stickel S., Sogin M.L. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 1988, 71:491-499.
17. Nylander J.A.A. MrModeltest 2.2. Program Distributed by the Author 2004, Evolutionary Biology Centre, Uppsala University, Uppsala.
18. Paiva T.S., Borges B.N., Silva-Neto I.D., Harada M.L. Morphology and 18S-rDNA phylogeny of Hemicycliostyla sphagni (Ciliophora, Hypotricha) from Brazil with redefinition of the genus Hemicycliostyla. Int. J. Syst. Evol. Microbiol. 2012, 62:229-241.
19. Ronquist F., Huelsenbeck J.P. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003, 19:1572-1574.
20. Shao C., Hu X., Al-Rasheid K.A.S., Song W., Warren A. Cell development of the marine spirotrichous ciliate Apokeronopsis crassa (Claparède & Lachmann, 1858) nov. comb. (Ciliophora: Stichotrichia), with the establishment of a new genus Apokeronopsis. J. Eukaryot. Microbiol. 2007, 54:392-401.
21. Shao C., Miao M., Li L., Song W., Al-Rasheid K.A.S., Ma H., Hu X., Chen Z. Morphogenesis and morphological redescription of a poorly known ciliate Apokeronopsis ovalis (Kahl, 1932) nov. comb. (Ciliophora: Urostylida). Acta Protozool. 2008, 47:363-376.
22. Shao C., Gao F., Hu X., Al-Rasheid K.A.S., Warren A. Ontogenesis and molecular phylogeny of a new marine urostylid ciliate, Anteholosticha petzi n. sp. (Ciliophora, Urostylida). J. Eukaryot. Microbiol. 2011, 58:254-265.
23. Shi X., Hu X., Warren A., Liu G. Redescription of morphology and morphogenesis of the freshwater ciliate. Pseudokeronopsis similis (Stokes, 1886) Borror and Wicklow 1983 (Ciliophora: Urostylida). Acta Protozool. 2007, 46:41-54.
24. Song W., Warren A., Roberts D., Wilbert N., Li L., Sun P., Hu X., Ma H. Comparison and redefinition of four marine, coloured Pseudokeronopsis spp. (Ciliophora, Hypotrichida), with emphasis on their living morphology. Acta Protozool. 2006, 45:271-287.
25. Song W., Wilbert N., Li L., Zhang Q. Re-evaluation on the diversity of the polyphyletic genus Metaurostylopsis (Ciliophora, Hypotricha): ontogenetic, morphologic, and molecular data suggest the establishment of a new genus Apourostylopsis nov. gen. J. Eukaryot. Microbiol. 2011, 58:11-21.
26. Wang Y., Hu X., Al-Rasheid K.A.S., Warren A. Two urostylid ciliates, Metaurostylopsis flavicana spec. nov. and Tunicothrix wilberti (Lin & Song, 2004) Xu et al., 2006 (Ciliophora, Stichotrichia), from a mangrove nature protection area in China. Int. J. Syst. Evol. Microbiol. 2011, 61:1740-1750.
27. Wilbert N. Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos 1975, 64:171-179.
28. Wilbert N., Song W. New contribution to the marine benthic ciliates from the Antarctic area, including description of seven new species (Protozoa, Ciliophora). J. Nat. Hist. 2005, 39:935-973.
29. Xu Y., Huang J., Hu X., Al-Rasheid K.A.S., Song W. Taxonomy, ontogeny and molecular phylogeny of Anteholosticha marimonilata spec. nov. (Ciliophora, Hypotrichida) from the Yellow Sea, China. Int. J. Syst. Evol. Microbiol. 2011, 61:2000-2014.
30. Yi Z., Song W. Evolution of the order Urostylida (Protozoa, Ciliophora): new hypotheses based on multi-gene information and identification of localized incongruence. PLoS ONE 2011, e1747a:1-12.
31. Yi Z., Song W., Stoeck T., Al-Rasheid K.A.S., Al-Khedhairy A.A., Gong J., Ma H., Chen Z. Phylogenetic analyses suggest that Psammomitra (Ciliophora, Urostylida) should represent an urostylid family, based on small subunit rRNA and alpha-tubulin gene sequence information. Zool. J. Linn. Soc. 2009, 157:227-236.
32. Yi Z., Dunthorn M., Song W., Stoeck T. Increased taxon sampling using both unidentified environmental sequences and identified cultures for microbial eukaryotes improves phylogenetic inference. Mol. Phylogenet. Evol. 2010, 57:937-941.
33. Zhang Q., Fan X., Clamp J., Al-Rasheid K.A.S., Song W. Description of Paratetrahymena parawassi n. sp. using morphological and molecular evidence and a phylogenetic analysis of Paratetrahymena and other taxonomically ambiguous genera in the order Loxocephalida (Ciliophora, Oligohymenophorea). J. Eukaryot. Microbiol. 2010, 57:483-493.