Preparation of Kupffer cell enriched non-parenchymal liver cells with high yield and reduced damage of surface markers by a modified method for flow cytometry

Cell Biology International

Volumn 37, Issue 4, 2013, Pages 284-291

  Xuy, Fan ^a   Zheny, Peng ^a   Zheng, Yu ^a   Lijuan, Feng ^a   Aiting, Yang ^a   Min, Cong ^a   Hong, You ^a   Jidong, Jia ^a  

^a CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

Collagenase perfusion; Flow cytometry; Kupffer cells isolation; Mean fluorescence intensity

Indexed keywords

CELL ANTIGEN; COLLAGENASE; F4/80 ANTIGEN; UNCLASSIFIED DRUG; BIOLOGICAL MARKER; DIFFERENTIATION ANTIGEN; MONOCYTE-MACROPHAGE DIFFERENTIATION ANTIGEN;

ANIMAL CELL; ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; CELL ISOLATION; CELL STRUCTURE; CELL SURFACE; CONTROLLED STUDY; ENZYME METABOLISM; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; KUPFFER CELL; LIVER NON PARENCHYMAL CELL; LIVER PARENCHYMA; LIVER PERFUSION; MALE; MEAN FLUORESCENCE INTENSITY; MOUSE; NONHUMAN; TEMPERATURE; ANIMAL; C57BL MOUSE; CELL SEPARATION; CHEMISTRY; CYTOLOGY; LIVER; METABOLISM; PERFUSION;

ANIMALS; ANTIGENS, DIFFERENTIATION; BIOLOGICAL MARKERS; CELL SEPARATION; COLLAGENASES; FLOW CYTOMETRY; KUPFFER CELLS; LIVER; MALE; MICE, INBRED C57BL; PERFUSION;

EID: 84875335617     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1002/cbin.10035     Document Type: Article

References (16)

1. Abuzakouk M, Feighery C, O'Farrelly C (1996) Collagenase and dispase enzymes disrupt lymphocyte surface molecules. J Immunol Methods 194(2): 211.
2. Bannikov GA, Lakritz J, Premanandan C, Mattoon JS, Abrahamsen EJ (2009) Kinetics of inhibition of purified bovine neutrophil matrix metalloproteinase 9 by low-molecular-weight inhibitors. Am J Vet Res 70(5): 633.
3. Baum B, Georgiou M (2011) Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling. J Cell Biol 192(6): 907.
4. Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol 43(3): 506.
5. Dong ZJ, Wei HM, Sun R, Tian ZG, Gao B 2004. Isolation of murine hepatic lymphocytes using mechanical dissection for phenotypic and functional analysis of NK1.1{thorn} cells. World J Gastroenterol 10(13): 1928.
6. Fain JN, Loken SC (1969) Response of trypsin-treated brown and white fat cells to hormones. Preferential inhibition of insulin action. J Biol Chem 244(13): 3500.
7. Geerts A, Niki T, Hellemans K, De Craemer D, Van Den Berg K, Lazou JM, Stange G, Van De Winkel M, De Bleser P (1998) Purification of rat hepatic stellate cells by side scatter-activated cell sorting. Hepatology 27(2): 590.
8. Gray DH, Chidgey AP, Boyd RL (2002) Analysis of thymic stromal cell populations using flow cytometry. J Immunol Methods 260 (1-2): 15.
9. Haubrich WS (2004) Kupffer of Kupffer cells. Gastroenterology 127(1):16
10. Janousek J, Strmen E, Gervais F (1993) Purification of murine Kupffer cells by centrifugal elutriation. J Immunol Methods 164(1): 109.
11. Katz SC, Pillarisetty VG, Bleier JI, Shah AB, DeMatteo RP (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells. J Immunol 173(1): 230.
12. Leung-Toung R, Zhao Y, Li W, Tam TF, Karimian K, Spino M (2006) Thiol proteases: inhibitors and potential therapeutic targets. Curr Med Chem 13(5): 547.
13. LiuW, Hou Y, Chen H,Wei H, LinW, Li J, Zhang M, He F, Jiang Y (2011) Sample preparation method for isolation of single-cell types from mouse liver for proteomic studies. Proteomics 11(17): 3556.
14. ten Hagen TL, van VW, Bakker-Woudenberg IA (1996) Isolation and characterization of murine Kupffer cells and splenic macrophages. J Immunol Methods 193(1): 81.
15. van Poll D, Nahmias Y, Soto-Gutierrez A, Ghasemi M, Yagi H, Kobayashi N, Yarmush ML, Hertl M (2010) Human immune reactivity against liver sinusoidal endothelial cells from GalTalpha(1,3)GalT-deficient pigs. Cell Transplant 19(6): 783.
16. Wu J,Meng Z, Jiang M, Zhang E, Trippler M, Broering R, Bucchi A, Krux F, Dittmer U, Yang D, Roggendorf M, Gerken G, Lu M, Schlaak JF (2010) Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology 129(3): 363.