Karyotype analysis of Panax ginseng C.A.Meyer, 1843 (Araliaceae) based on rDNA loci and DAPI band distribution

Comparative Cytogenetics

Volumn 6, Issue 4, 2012, Pages 425-441

  Waminal, Nomar Espinosa ^a,b   Park, Hye Mi ^a,b   Ryu, Kwang Bok ^b,c   Kim, Joo Hyung ^b   Yang, Tae Jin ^a   Kim, Hyun Hee ^b  

^a Seoul National University   (South Korea)

^b Sahmyook University   (South Korea)

^c Kyungpook National University   (South Korea)

Author keywords

45S; 5SrDNA; Araliaceae; DAPI band; FISH; Panax ginseng; rDNA

Indexed keywords

EID: 84875278660     PISSN: 19930771     EISSN: 1993078X     Source Type: Journal    
DOI: 10.3897/CompCytogen.v6i4.3740     Document Type: Article

References (63)

1. Ali HB, Lysak MA, Schubert I (2005) Chromosomal localization of rDNA in the Brassicaceae. Genome 48: 341-346. doi: 10.1139/g04-116
2. Ananiev EV, Phillips RL, Rines HW (1998) Complex structure of knob DNA on maize chromosome 9: retrotransposon invasion into heterochromatin. Genetics 149: 2025-2037.
3. Blair A (1975) Karyotypes of five plant species with disjunct distributions in Virginia and the Carolinas. American Journal of Botany 62: 833-837. doi: 10.2307/2441894
4. Capdeville G, Souza Júnior MT, Szinay D, Diniz LEC, Wijnker E, Swennen R, Kema GHJ, Jong H (2008) The potential of high-resolution BAC-FISH in banana breeding. Euphytica 166: 431-443. doi: 10.1007/s10681-008-9830-2
5. Chen JF, Staub JE, Adelberg JW, Jiang J (1999) Physical mapping of 45S rRNA genes in Cucumis species by fluorescence in situ hybridization. Canadian Journal of Botany 77: 389-393.
6. Chester M, Gallagher JP, Symonds VV, da Silva AVC, Mavrodiev EV, Leitch AR, Soltis PS, Soltis DE (2012) Extensive chromosomal variation in a recently formed natural allopolyploid species, Tragopogon miscellus (Asteraceae). Proceedings of the National Academy of Sciences 109: 1176-1181. doi: 10.1073/pnas.1112041109
7. Choi HW, Choi DH, Bang KH, Paek KY, Seong NS, Bak JW (2009) FISH and GISH analysis of the genomic relationships among Panax species. Genes & Genomics 31: 99-105. doi: 10.1007/BF03191143
8. Choi HI, Kim NH, Kim JH, Choi BS, Ahn IO, Lee JS, Yang TJ (2011) Development of reproducible EST-derived SSR markers and assessment of genetic diversity in Panax ginseng cultivars and related species. Journal of Ginseng Research 35: 399-412. doi: 10.5142/jgr.2011.35.4.399
9. Costa Silva S, Marques A, Santos Soares Filho W, Mirkov TE, Pedrosa-Harand A, Guerra M (2011) The cytogenetic map of the Poncirus trifoliata (L.) Raf.-a nomenclature system for chromosomes of all citric species. Tropical Plant Biology 4: 99-105. doi: 10.1007/s12042-011-9072-7
10. Court WE (2000) Ginseng: The genus Panax. Hardwood Academic Publishers, Amsterdam, Netherlands, 249 pp.
11. Darlington C, Wylie A (1956) Chromosome atlas of flowering plants. The Macmillan Company, New York, USA, 519 pp.
12. Eriksson S, Kim SK, Kubista M, Norden B (1993) Binding of 4', 6-diamidino-2-phenylindole (DAPI) to AT regions of DNA: evidence for an allosteric conformational change. Biochemistry 32: 2987-2998. doi: 10.1021/bi00063a009
13. Fransz PF, Stam M, Montijn B, Hoopen RT, Wiegant J, Kooter JM, Oud O, Nanninga N (1996) Detection of single-copy genes and chromosome rearrangements in Petunia hybrida by fluorescence in situ hybridization. The Plant Journal 9: 767-774. doi: 10.1046/j.1365- 313X.1996.9050767.x
14. Gerlach W, Bedbrook J (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Research 7: 1869-1885. doi: 10.1093/nar/7.7.1869
15. Graham SA (1966) The genera of Araliaceae in the southeastern United States. Journal of the Arnold Arboretum 47: 126-136.
16. Han Y, Zhang Z, Liu C, Liu J, Huang S, Jiang J, Jin W (2009) Centromere repositioning in cucurbit species: implication of the genomic impact from centromere activation and inactivation. Proceedings of the National Academy of Sciences 106: 14937-14941. doi: 10.1073/pnas.0904833106
17. Heng HHQ, Tsui LC (1993) Modes of DAPI banding and simultaneous in situ hybridization. Chromosoma 102: 325-332. doi: 10.1007/BF00661275
18. Heslop-Harrison JS, Schwarzacher T (2011) Organisation of the plant genome in chromosomes. The Plant Journal 66: 18-33. doi: 10.1111/j.1365-313X.2011.04544.x
19. Ho IS, Leung FC (2002) Isolation and characterization of repetitive DNA sequences from Panax ginseng. Molecular Genetics and Genomics 266: 951-961. doi: 10.1007/s00438- 001-0617-6
20. Hong C, Lee S, Park J, Plaha P, Park Y, Lee Y, Choi J, Kim K, Lee J, Jin H, Choi S, Lim Y (2004) Construction of a BAC library of Korean ginseng and initial analysis of BAC-end sequences. Molecular Genetics and Genomics 271: 709-716. doi: 10.1007/s00438-004-1021-9
21. Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen EA, Wu Y, Guo J, He J, Jia Z, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Fang L, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Li S, Zhang X, Wang J, Sun R, Zhang B, Jiang S, Du Y (2009) The genome of the cucumber, Cucumis sativus L. Nature Genetics 41: 1275-1281. doi: 10.1038/ng.475
22. Hwang Y, Kim H, Kwon S, Yang T, Ko H, Park B, Chung J, Lim K (2009) Karyotype analysis of three Brassica species using five different repetitive DNA markers by fluorescence in situ hybridization. Horticulture, Environment, and Biotechnology 27: 456-463.
23. Kato A, Lamb JC, Birchler JA (2004) Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proceedings of the National Academy of Sciences 101: 13554-13559. doi: 10.1073/pnas.0403659101
24. Kharb P, Dong J, Islam-Faridi M, Stelly DM, Hall TC (2001) Fluorescence in situ hybridization of single copy transgenes in rice chromosomes. In Vitro Cellular & Developmental Biology-Plant 37: 1-5. doi: 10.1007/s11627-001-0001-6
25. Kim NH, Choi HI, Ahn IO, Yang TJ (2012a) EST-SSR Marker Sets for Practical Authentication of All Nine Registered Korean Ginseng Cultivars. Journal of Ginseng Research 36. doi: 10.5142/jgr.2012.36.3.000
26. Kim J, Jung J-Y, Choi H-I, Kim N-H, Park J, Lee Y, Yang T-J (2012b) Diversity and evolution of major Panax species revealed by scanning the entire chloroplast intergenic spacer sequences. Genetic Resources and Crop Evolution: 1-13. doi: 10.1007/s10722-012-9844-4
27. Ko KM, Song JJ, Hwang B, Kang YH (1993) Cytogenetic and histological characteristics of ginseng hairy root transformed by Agrobacterium rhizogenes. Korean Journal of Botany 36: 75-81.
28. Kolano B, Siwinska D, Maluszynska J (2008) Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg. Acta Societatis Botanicorum Poloniae 77: 293-298.
29. Kovarik A, Dadejova M, Lim YK, Chase MW, Clarkson JJ, Knapp S, Leitch AR (2008) Evolution of rDNA in Nicotiana allopolyploids: a potential link between rDNA homogenization and epigenetics. Annals of Botany 101: 815-823. doi: 10.1093/aob/mcn019
30. Kovarik A, Pires JC, Leitch AR, Lim KY, Sherwood AM, Matyasek R, Rocca J, Soltis DE, Soltis PS (2005) Rapid concerted evolution of nuclear ribosomal DNA in two Tragopogon allopolyploids of recent and recurrent origin. Genetics 169: 931-944. doi: 10.1534/genetics. 104.032839
31. Kubota Y, Kubota K, Tani S (2000) DNA binding properties of DAPI (4',6-diamidino-2-phenylindole) analogues having an imidazole ring or a tetrahydropyrimidine ring: Groovebinding and intercalation. Nucleic Acids Symposium: 53-54
32. Lamb JC, Meyer JM, Corcoran B, Kato A, Han F, Birchler JA (2007) Distinct chromosomal distributions of highly repetitive sequences in maize. Chromosome Research 15: 33-49. doi: 10.1007/s10577-006-1102-1
33. Lan T, Albert VA (2011) Dynamic distribution patterns of ribosomal DNA and chromosomal evolution in Paphiopedilum, a lady's slipper orchid. BMC Plant Biology 11: 126. doi: 10.1186/1471-2229-11-126
34. Leflon M, Eber F, Letanneur JC, Chelysheva L, Coriton O, Huteau V, Ryder CD, Barker G, Jenczewski E, Chevre AM (2006) Pairing and recombination at meiosis of Brassica rapa (AA) x Brassica napus (AACC) hybrids. Theoretical and Applied Genetics 113: 1467-1480. doi: 10.1007/s00122-006-0393-0
35. Leung KW, Wong AS (2010) Pharmacology of ginsenosides: a literature review. Chinese Medicine 5: 20. doi: 10.1186/1749-8546-5-20
36. Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201-220. doi: 10.1111/j.1601-5223.1964.tb01953.x
37. Lim KB, de Jong H, Yang TJ, Park JY, Kwon SJ, Kim JS, Lim MH, Kim JA, Jin M, Jin YM, Kim SH, Lim YP, Bang JW, Kim HI, Park BS (2005) Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa. Molecules and Cells 19: 436-444.
38. Maluszynska J, Heslop-Harrison JS (1991) Localization of tandemly repeated DNA sequences in Arabidopsis thaliana. The Plant Journal 1: 159-166. doi: 10.1111/j.1365- 313X.1991.00159.x
39. Mendes MM, da Rosa R, Giuliano-Caetano L, Dias AL (2011) Karyotype diversity of four species of the incertae sedis group (Characidae) from different hydrographic basins: analysis of AgNORs, CMA3 and 18S rDNA. Genetics and Molecular Research 10: 3596-3608. doi: 10.4238/2011.November.22.5
40. Ngan F, Shaw P, But P, Wang J (1999) Molecular authentication of Panax species. Phytochemistry 50: 787-791. doi: 10.1016/s0031-9422(98)00606-2
41. Ozkan H, Tuna M, Arumuganathan K (2003) Nonadditive changes in genome size during allopolyploidization in the wheat (Aegilops-Triticum) group. Journal of Heredity 94: 260- 264. doi: 10.1093/jhred/esg053
42. Park M, Park J, Kim S, Kwon JK, Park HM, Bae IH, Yang TJ, Lee YH, Kang BC, Choi D (2012) Evolution of the large genome in Capsicum annuum occurred through accumulation of single-type long terminal repeat retrotransposons and their derivatives. The Plant Journal 69: 1018-1029. doi: 10.1111/j.1365-313X.2011.04851.x
43. Peacock WJ, Dennis ES, Rhoades MM, Pryor AJ (1981) Highly repetitive DNA sequence limited to knob heterochromatin in maize. Proceedings of the National Academy of Sciences 78: 4490-4494.
44. Pellicer J, Garnatje T, Hidalgo O, Tagashira N, Valles J, Kondo K (2010) Do polyploids require proportionally less rDNA loci than their corresponding diploids? Examples from Artemisia subgenera Absinthium and Artemisia (Asteraceae, Anthemideae). Plant Biosystems 144: 841-848. doi: 10.1080/11263504.2010.522783
45. Pinto MMPD, Calixto MD, de Souza MJ, de Araujo APT, Langguth A, Santos N (2012) Cytotaxonomy of the subgenus Artibeus (Phyllostomidae, Chiroptera) by characterization of species-specific markers. Comparative Cytogenetics 6: 17-28. doi: 10.3897/CompCytogen. v6i1.1510
46. Sadder MT, Webber G (2001) Karyotype of maize (Zea mays L.) mitotic metaphase chromosomes as revealed by fluorescence in situ hybridization (FISH) with cytogenetic DNA markers. Plant Molecular Biology Reporter 19: 117-123. doi: 10.1007/BF02772153
47. Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307-324. doi: 10.1007/BF00292840
48. Snowdon RJ (2007) Cytogenetics and genome analysis in Brassica crops. Chromosome Research 15: 85-95. doi: 10.1007/s10577-006-1105-y
49. Snowdon RJ, Köhler W, Köhler A (1997) Chromosomal localization and characterization of rDNA loci in the Brassica A and C genomes. Genome 40: 582-587. doi: 10.1139/g97-076
50. Topp CN, Okagaki RJ, Melo JR, Kynast RG, Phillips RL, Dawe RK (2009) Identification of a maize neocentromere in an oat-maize addition line. Cytogenetic and Genome Research 124: 228-238. doi: 10.1159/000218128
51. Vasconcelos S, Souza AA, Gusmao CL, Milani M, Benko-Iseppon AM, Brasileiro-Vidal AC (2010) Heterochromatin and rDNA 5S and 45S sites as reliable cytogenetic markers for castor bean (Ricinus communis, Euphorbiaceae). Micron 41: 746-753. doi: 10.1016/j.micron. 2010.06.002
52. Waminal NE, Kim HH (2012) Dual-color FISH karyotype and rDNA distribution analyses on four Cucurbitaceae species. Horticulture, Environment, and Biotechnology 53: 49-56. doi: 10.1007/s13580-012-0105-4
53. Waminal NE, Kim N-S, Kim HH (2011) Dual-color FISH karyotype analyses using rDNAs in three Cucurbitaceae species. Genes & Genomics 33: 521-528. doi: 10.1007/s13258- 011-0046-9
54. Wang K, Guo W, Zhang T (2007) Detection and mapping of homologous and homoeologous segments in homoeologous groups of allotetraploid cotton by BAC-FISH. BMC Genomics 8: 178. doi: 10.1186/1471-2164-8-178
55. Wen J, Zimmer EA (1996) Phylogeny and Biogeography of Panax L. (the Ginseng Genus, Araliaceae): inference from ITS sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution 6: 167-177. doi: 10.1006/mpev.1996.0069
56. Wendel JF (2000) Genome evolution in polyploids. Plant Molecular Biology 42: 225-249. doi: 10.1023/A:1006392424384
57. Xiang-Hui J (2011) Karyotype analysis of three Solanum plants using combined PI-DAPI staining and double fluorescence in situ hybridization with 45S and 5S rDNA probes. African Journal of Biotechnology 10: 18948-18957. doi: 10.5897/ajb09.1874
58. Xiong ZY, Pires JC (2011) Karyotype and identification of all homoeologous chromosomes of allopolyploid Brassica napus and Its diploid progenitors. Genetics 187: 37-49. doi: 10.1534/genetics.110.122473
59. Yang D-Q (1981) The cyto-taxonomic studies on some species of Panax L. Acta Phytotaxonomica Sinica 19: 298-303.
60. Yi T, Lowry PP, Plunkett GM, Wen J (2004) Chromosomal evolution in Araliaceae and close relatives. Taxon 53: 987-1005. doi: 10.2307/4135565
61. Yoshikazu H, Matoba H, Kondo K (2006) Physical mapping of ribosomal RNA genes in the genus Artemisia L. (Asteraceae). Caryologia 59: 312-318.
62. Yuan H, Kim S, Quan H, Huang B, Chung S (2010) Ginseng leaf extract prevents high fat diet-induced hyperglycemia and hyperlipidemia through AMPK activation. Journal of Ginseng Research 34: 369-375. doi: 10.5142/jgr.2010.34.4.369
63. Zhang J, Bae T, Boo K, Sun H, Song I, Pham C, Ganesan M, Yang D, Kang H, Ko S, Riu K, Lim P, Lee H (2011) Ginsenoside production and morphological characterization of wild ginseng (Panax ginseng Meyer) mutant lines induced by g-irradiation (60Co) of adventitious roots. Journal of Ginseng Research 35: 283-293. doi: 10.5142/jgr.2011.35.3.283