A new flow cytometry method to measure oxidative status: The Peroxidation of Leukocytes Index Ratio (PLIR)

Journal of Immunological Methods

Volumn 390, Issue 1-2, 2013, Pages 113-120

  Peluso, Ilaria ^a   Adorno, Gaspare ^b   Raguzzini, Anna ^a   Urban, Lourdes ^a   Ghiselli, Andrea ^a   Serafini, Mauro ^a  

^a ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

Immune metabolism; Immune nutrition; Oxidative stress; Peroxidation of Leukocytes Index Ratio (PLIR)

Indexed keywords

CHOLESTEROL; GLUCOSE; INSULIN; PHORBOL 13 ACETATE 12 MYRISTATE; REACTIVE OXYGEN METABOLITE; THIOL DERIVATIVE; TRIACYLGLYCEROL; TROLOX C; URIC ACID;

ADULT; ANTIOXIDANT ACTIVITY; ARTICLE; BLEACHING; CHOLESTEROL BLOOD LEVEL; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GLUCOSE BLOOD LEVEL; HUMAN; HUMAN CELL; INSULIN BLOOD LEVEL; LEUKOCYTE; LYMPHOCYTE; MALE; MONOCYTE; NEUTROPHIL; OXIDATION REDUCTION STATE; OXIDATIVE STRESS; PEROXIDATION; PEROXIDATION OF LEUKOCYTES INDEX RATIO; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; TRIACYLGLYCEROL BLOOD LEVEL;

ADULT; AMIDINES; ANTIOXIDANTS; AZA COMPOUNDS; CELL MEMBRANE; CHOLESTEROL; CHROMANS; FATTY ACIDS; FEMALE; FLOW CYTOMETRY; HUMANS; LEUKOCYTES; LYMPHOCYTES; MALE; MIDDLE AGED; MONOCYTES; NEUTROPHILS; OXIDATION-REDUCTION; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; REPRODUCIBILITY OF RESULTS; TETRADECANOYLPHORBOL ACETATE;

EID: 84875274201     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.02.005     Document Type: Article

References (34)

1. Albers R., Antoine J.M., Bourdet-Sicard R., Calder P.C., Gleeson M., Lesourd B., Samartín S., Sanderson I.R., Van Loo J., Vas Dias F.W., Watzl B. Markers to measure immunomodulation in human nutrition intervention studies. Br. J. Nutr. 2005, 94(3):452.
2. Benzie I.F.F., Strain J.J. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal. Biochem. 1996, 239:70.
3. Bloomer R.J. Decreased blood antioxidant capacity and increased lipid peroxidation in young cigarette smokers compared to nonsmokers: impact of dietary intake. Nutr. J. 2007, 6:39.
4. Brouwers J.F., Gadella B.M. In situ detection and localization of lipid peroxidation in individual bovine sperm cells. Free Radic. Biol. Med. 2003, 35(11):1382.
5. Calder P.C., Albers R., Antoine J.M., Blum S., Bourdet-Sicard R., Ferns G.A., Folkerts G., Friedmann P.S., Frost G.S., Guarner F., Løvik M., Macfarlane S., Meyer P.D., M'Rabet L., Serafini M., van Eden W., van Loo J., Vas Dias W., Vidry S., Winklhofer-Roob B.M., Zhao J. Inflammatory disease processes and interactions with nutrition. Br. J. Nutr. 2009, 101(Suppl. 1):S1.
6. Calder P.C., Ahluwalia N., Brouns F., Buetler T., Clement K., Cunningham K., Esposito K., Jönsson L.S., Kolb H., Lansink M., Marcos A., Margioris A., Matusheski N., Nordmann H., O'Brien J., Pugliese G., Rizkalla S., Schalkwijk C., Tuomilehto J., Wärnberg J., Watzl B., Winklhofer-Roob B.M. Dietary factors and low-grade inflammation in relation to overweight and obesity. Br. J. Nutr. 2011, 106(Suppl. 3):S5.
7. Candore G., Caruso C., Jirillo E., Magrone T., Vasto S. Low grade inflammation as a common pathogenetic denominator in age-related diseases: novel drug targets for anti-ageing strategies and successful ageing achievement. Curr. Pharm. Des. 2010, 16(6):584.
8. Caro A.A., Thompson S., Tackett J. Increased oxidative stress and cytotoxicity by hydrogen sulfide in HepG2 cells overexpressing cytochrome P450 2E1. Cell Biol. Toxicol. 2011, 27(6):439.
9. Cuddihy S.L., Parker A., Harwood D.T., Vissers M.C., Winterbourn C.C. Ascorbate interacts with reduced glutathione to scavenge phenoxyl radicals in HL60 cells. Free Radic. Biol. Med. 2008, 44(8):1637.
10. Dombrecht E.J., De Tollenaere C.B., Aerts K., Cos P., Schuerwegh A.J., Bridts C.H., Van Offel J.F., Ebo D.G., Stevens W.J., De Clerck L.S. Antioxidant effect of bisphosphonates and simvastatin on chondrocyte lipid peroxidation. Biochem. Biophys. Res. Commun. 2006, 348(2):459.
11. Domínguez-Rebolledo Á.E., Martínez-Pastor F., Fernández-Santos M.R., del Olmo E., Bisbal A., Ros-Santaella J.L., Garde J.J. Comparison of the TBARS assay and BODIPY C11 probes for assessing lipid peroxidation in red deer spermatozoa. Reprod. Domest. Anim. 2010, 45(6):e360.
12. Drummen G.P., van Liebergen L.C., Op den Kamp J.A., Post J.A. C11-BODIPY(581/591), an oxidation-sensitive fluorescent lipid peroxidation probe: (micro)spectroscopic characterization and validation of methodology. Free Radical Biol. Med. 2002, 33:473.
13. Ellman G.L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 1959, 82:70.
14. Ghiselli A., Serafini M., Maiani G., Azzini E., Ferro-Luzzi A. A fluorescence-based method for measuring total plasma antioxidant capability. Free Radic. Biol. Med. 1995, 18:29.
15. Hoffmann F.W., Hashimoto A.C., Shafer L.A., Dow S., Berry M.J., Hoffmann P.R. Dietary selenium modulates activation and differentiation of CD4+ T cells in mice through a mechanism involving cellular free thiols. J. Nutr. 2010, 140(6):1155.
16. Iwase H., Robin E., Guzy R.D., Mungai P.T., Vanden Hoek T.L., Chandel N.S., Levraut J., Schumacker P.T. Nitric oxide during ischemia attenuates oxidant stress and cell death during ischemia and reperfusion in cardiomyocytes. Free Radic. Biol. Med. 2007, 43(4):590.
17. Jofre-Monseny L., de Pascual-Teresa S., Plonka E., Huebbe P., Boesch-Saadatmandi C., Minihane A.M., Rimbach G. Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages. Br. J. Nutr. 2007, 97(5):864.
18. Kavouras S.A., Panagiotakos D.B., Pitsavos C., Chrysohoou C., Arnaoutis G., Skoumas Y., Stefanadis C. Physical activity and adherence to mediterranean diet increase total antioxidant capacity: the ATTICA Study. Cardiol. Res. Pract. 2010, 2011:248626.
19. Levraut J., Iwase H., Shao Z.H., Vanden Hoek T.L., Schumacker P.T. Cell death during ischemia: relationship to mitochondrial depolarization and ROS generation. Am. J. Physiol. Heart Circ. Physiol. 2003, 284(2):H549.
20. Magrone T., Jirillo E. Mechanisms of neutrophil-mediated disease: innovative therapeutic interventions. Curr. Pharm. Des. 2012, 18(12):1609.
21. Noritake M., Katsura Y., Shinomiya N., Kanatani M., Uwabe Y., Nagata N., Tsuru S. Intracellular hydrogen peroxide production by peripheral phagocytes from diabetic patients. Dissociation between polymorphonuclear leucocytes and monocytes. Clin. Exp. Immunol. 1992, 88(2):269.
22. Packer L., Glazer A.N. Oxygen Radicals in Biological Systems 1990, Academic Press, San Diego.
23. Pap E.H., Drummen G.P., Winter V.J., Kooij T.W., Rijken P., Wirtz K.W., Op den Kamp J.A., Hage W.J., Post J.A. Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(581/591). FEBS Lett. 1999, 453:278.
24. Partyka A., Lukaszewicz E., Nizański W., Twardoń J. Detection of lipid peroxidation in frozen-thawed avian spermatozoa using C(11)-BODIPY(581/591). Theriogenology 2011, 75(9):1623.
25. Peluso I., Morabito G., Riondino S., La Farina F., Serafini M. Lymphocytes as internal standard in oxidative burst analysis by cytometry: a new data analysis approach. J. Immunol. Methods 2012, 379(1-2):61.
26. Peluso I., Morabito G., Urban L., Ioannone F., Serafini M. Oxidative stress in atherosclerosis development: the central role of LDL and oxidative burst. Endocr. Metab. Immune Disord. Drug Targets 2012, (Oct 8. [Epub ahead of print]).
27. Scorletti E., Calder P.C., Byrne C.D. Non-alcoholic fatty liver disease and cardiovascular risk: metabolic aspects and novel treatments. Endocrine 2011, 40(3):332.
28. Serafini M., Miglio C., Peluso I., Petrosino T. Modulation of plasma non enzimatic antioxidant capacity (NEAC) by plant foods: the role of polyphenols. Curr. Top. Med. Chem. 2011, 11(14):1821.
29. Shi Y., Evans J.E., Rock K.L. Molecular identification of a danger signal that alerts the immune system to dying cells. Nature 2003, 425:516.
30. Varela N.M., Quiñones L.A., Orellana M., Poniachik J., Csendes A., Smok G., Rodrigo R., Cáceres D.D., Videla L.A. Study of cytochrome P450 2E1 and its allele variants in liver injury of nondiabetic, nonalcoholic steatohepatitis obese women. Biol. Res. 2008, 41(1):81.
31. Vowells S.J., Sekhsaria S., Malech H.L., Shalit M., Fleisher T.A. Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes. J. Immunol. Methods 1995, 178:89.
32. Walrand S., Valeix S., Rodriguez C., Ligot P., Chassagne J., Vasson M.P. Flow cytometry study of polymorphonuclear neutrophil oxidative burst: a comparison of three fluorescent probes. Clin. Chim. Acta 2003, 331(1-2):103.
33. Webb A.L., Villamor E. Update: effects of antioxidant and non-antioxidant vitamin supplementation on immune function. Nutr. Rev. 2007, 65(5):181.
34. Yokoyama H., Emoto M., Fujiwara S., Motoyama K., Morioka T., Komatsu M., Tahara H., Shoji T., Okuno Y., Nishizawa Y. Quantitative insulin sensitivity check index and the reciprocal index of homeostasis model assessment in normal range weight and moderately obese type 2 diabetic patients. Diabetes Care 2003, 26(8):2426.