Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42

Biochemical and Biophysical Research Communications

Volumn 432, Issue 2, 2013, Pages 302-307

  Fernández, Darío ^b   Horrillo, Angélica ^b   Alquezar, Carolina ^a   González Manchón, Consuelo ^a,b   Parrilla, Roberto ^a,b   Ayuso, Matilde S ^a,b  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b CENTRO DE INVESTIGACIÓN BIOMÉDICA EN RED DE ENFERMEDADES RARAS CIBERER   (Spain)

Author keywords

Cdc42; Cell adhesion; Cell migration; CHO cells; Podocalyxin; Rac1

Indexed keywords

CORTACTIN; EZRIN; GUANOSINE TRIPHOSPHATASE; PODOCALYXIN; PROTEIN CDC42; RAC1 PROTEIN; SIGNAL PEPTIDE;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; CELL LYSATE; CELL MIGRATION; DELETION MUTANT; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL ASSAY; CDC42 GTP-BINDING PROTEIN; CELL ADHESION; CELL MOVEMENT; CHO CELLS; CRICETINAE; GENE SILENCING; HUMANS; RAC1 GTP-BINDING PROTEIN; SEQUENCE DELETION; SIALOGLYCOPROTEINS; WOUND HEALING;

EID: 84875245014     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.01.112     Document Type: Article

References (32)

1. Michael A.F., Blau E., Vernier R.L. Glomerular polyanion. Alteration in aminonucleoside nephrosis. Lab. Invest. 1970, 23:649-657.
2. Kerjaschki D., Vernillo A.T., Farquhar M.G. Reduced sialylation of podocalyxin - the major sialoprotein of the rat kidney glomerulus - in aminonucleoside nephrosis. Am. J. Pathol. 1985, 118:343-349.
3. Dekan G., Gabel C., Farquhar M.G. Sulfate contributes to the negative charge of podocalyxin, the major sialoglycoprotein of the glomerular filtration slits. Proc. Natl. Acad. Sci. USA 1991, 88:5398-5402.
4. Kershaw D.B., Thomas P.E., Wharram B.L., Goyal M., Wiggins J.E., Whiteside C.I., Wiggins R.C. Molecular cloning, expression, and characterization of podocalyxin-like protein 1 from rabbit as a transmembrane protein of glomerular podocytes and vascular endothelium. J. Biol. Chem. 1995, 270:29439-29446.
5. Kershaw D.B., Beck S.G., Wharram B.L., Wiggins J.E., Goyal M., Thomas P.E., Wiggins R.C. Molecular cloning and characterization of human podocalyxin-like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin. J. Biol. Chem. 1997, 272:15708-15714.
6. Somlo S., Mundel P. Getting a foothold in nephrotic syndrome. Nat. Genet. 2000, 24:333-335.
7. Mundel P., Shankland S.J. Podocyte biology and response to injury. J. Am. Soc. Nephrol. 2002, 13:3005-3015.
8. Zheng M., Lv L.L., Ni J., Ni H.F., Li Q., Ma K.L., Liu B.C. Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. PLoS One 2011, 6:e20431.
9. Hara M., Yanagihara T., Takada T., Itoh M., Adachi Y., Yoshizumi A., Kawasaki K., Yamamoto T., Kihara I. Podocalyxin on the glomerular epithelial cells is preserved well in various glomerular diseases. Nephron 1994, 67:123-124.
10. Schnabel E., Dekan G., Miettinen A., Farquhar M.G. Biogenesis of podocalyxin - the major glomerular sialoglycoprotein - in the newborn rat kidney. Eur. J. Cell. Biol. 1989, 48:313-326.
11. Schnabel E., Anderson J.M., Farquhar M.G. The tight junction protein ZO-1 is concentrated along slit diaphragms of the glomerular epithelium. J. Cell. Biol. 1990, 111:1255-1263.
12. Doyonnas R., Kershaw D.B., Duhme C., Merkens H., Chelliah S., Graf T., McNagny K.M. Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin. J. Exp. Med. 2001, 194:13-27.
13. Takeda T., Go W.Y., Orlando R.A., Farquhar M.G. Expression of podocalyxin inhibits cell-cell adhesion and modifies junctional properties in Madin-Darby canine kidney cells. Mol. Biol. Cell. 2000, 11:3219-3232.
14. Kerosuo L., Juvonen E., Alitalo R., Gylling M., Kerjaschki D., Miettinen A. Podocalyxin in human haematopoietic cells. Br. J. Haematol. 2004, 124:809-818.
15. Kershaw D.B., Wiggins J.E., Wharram B.L., Wiggins R.C. Assignment of the human podocalyxin-like protein (PODXL) gene to 7q32-q33. Genomics 1997, 45:239-240.
16. Schopperle W.M., Kershaw D.B., DeWolf W.C. Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin. Biochem. Biophys. Res. Commun. 2003, 300:285-290.
17. Somasiri A., Nielsen J.S., Makretsov N., McCoy M.L., Prentice L., Gilks C.B., Chia S.K., Gelmon K.A., Kershaw D.B., Huntsman D.G., McNagny K.M., Roskelley C.D. Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression. Cancer Res. 2004, 64:5068-5073.
18. Casey G., Neville P.J., Liu X., Plummer S.J., Cicek M.S., Krumroy L.M., Curran A.P., McGreevy M.R., Catalona W.J., Klein E.A., Witte J.S. Podocalyxin variants and risk of prostate cancer and tumor aggressiveness. Hum. Mol. Genet. 2006, 15:735-741.
19. Larrucea S., Butta N., Rodriguez R.B., Alonso-Martin S., Arias-Salgado E.G., Ayuso M.S., Parrilla R. Podocalyxin enhances the adherence of cells to platelets. Cell. Mol. Life Sci. 2007, 64:2965-2974.
20. Larrucea S., Butta N., Arias-Salgado E.G., Alonso-Martin S., Ayuso M.S., Parrilla R. Expression of podocalyxin enhances the adherence, migration, and intercellular communication of cells. Exp. Cell. Res. 2008, 314:2004-2015.
21. Pericacho M., Alonso-Martin S., Larrucea S., Gonzalez-Manchon C., Fernandez D., Sanchez I., Ayuso M.S., Parrilla R. Diminished thrombogenic responses by deletion of the Podocalyxin Gene in mouse megakaryocytes. PLoS One 2011, 6:e26025.
22. Sassetti C., Tangemann K., Singer M.S., Kershaw D.B., Rosen S.D. Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34. J. Exp. Med. 1998, 187:1965-1975.
23. Hsu Y.H., Lin W.L., Hou Y.T., Pu Y.S., Shun C.T., Chen C.L., Wu Y.Y., Chen J.Y., Chen T.H., Jou T.S. Podocalyxin EBP50 ezrin molecular complex enhances the metastatic potential of renal cell carcinoma through recruiting Rac1 guanine nucleotide exchange factor ARHGEF7. Am. J. Pathol. 2010, 176:3050-3061.
24. Nielsen J.S., McNagny K.M. Novel functions of the CD34 family. J. Cell. Sci. 2008, 121:3683-3692.
25. Economou C.G., Kitsiou P.V., Tzinia A.K., Panagopoulou E., Marinos E., Kershaw D.B., Kerjaschki D., Tsilibary E.C. Enhanced podocalyxin expression alters the structure of podocyte basal surface. J. Cell. Sci. 2004, 117:3281-3294.
26. Cipollone J.A., Graves M.L., Kobel M., Kalloger S.E., Poon T., Gilks C.B., McNagny K.M., Roskelley C.D. The anti-adhesive mucin podocalyxin may help initiate the transperitoneal metastasis of high grade serous ovarian carcinoma. Clin. Exp. Metastasis 2012, 29:239-252.
27. Fernandez D., Larrucea S., Nowakowski A., Pericacho M., Parrilla R., Ayuso M.S. Release of podocalyxin into the extracellular space. Role of metalloproteinases. Biochim. Biophys. Acta 1813, 2011:1504-1510.
28. +-exchanger regulatory factor 2 and ezrin. Clin. Exp. Nephrol. 2003, 7:260-269.
29. Kobayashi T., Notoya M., Shinosaki T., Kurihara H. Cortactin interacts with podocalyxin and mediates morphological change of podocytes through its phosphorylation. Nephron Exp. Nephrol. 2009, 113:e89-e96.
30. Kirkbride K.C., Sung B.H., Sinha S., Weaver A.M. Cortactin: a multifunctional regulator of cellular invasiveness. Cell. Adh. Migr. 2011, 5:187-198.
31. Schmieder S., Nagai M., Orlando R.A., Takeda T., Farquhar M.G. Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells. J. Am. Soc. Nephrol. 2004, 15:2289-2298.
32. Furness S.G., McNagny K. Beyond mere markers: functions for CD34 family of sialomucins in hematopoiesis. Immunol. Res. 2006, 34:13-32.