A coculture system of cavernous endothelial and smooth muscle cells

International Journal of Impotence Research

Volumn 25, Issue 2, 2013, Pages 63-68

  Ning, H ^a   Lin, G ^a   Lue, T F ^a   Lin, C S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

cavernous endothelial cells; cavernous smooth muscle cells; coculture; diabetes; erectile dysfunction; high glucose

Indexed keywords

CD31 ANTIGEN; COLLAGEN TYPE 4; ENDOTHELIAL NITRIC OXIDE SYNTHASE; GLUCOSE; LOW DENSITY LIPOPROTEIN; VON WILLEBRAND FACTOR;

ARTICLE; CELL STRUCTURE; COCULTURE; CONTROLLED STUDY; CORPUS CAVERNOSUM; DIABETES MELLITUS; ENDOTHELIUM CELL; ERECTILE DYSFUNCTION; EXPLANT; FIBROBLAST; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GLUCOSE BLOOD LEVEL; HUMAN; HUMAN CELL; PENIS PROSTHESIS; PHENOTYPE; PREPUCE; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SMOOTH MUSCLE FIBER;

ANTIGENS, CD31; COCULTURE TECHNIQUES; DIABETES COMPLICATIONS; ENDOTHELIAL CELLS; ERECTILE DYSFUNCTION; FEASIBILITY STUDIES; GLUCOSE; HUMANS; MALE; MYOCYTES, SMOOTH MUSCLE; PENIS;

EID: 84875054802     PISSN: 09559930     EISSN: 14765489     Source Type: Journal    
DOI: 10.1038/ijir.2012.36     Document Type: Article

References (20)

1. Lin G, Qiu X, Fandel TM, Albersen M, Wang Z, Lue TF et al. Improved penilehistology by phalloidin stain: circular and longitudinal cavernous smooth muscles,dual-endothelium arteries, and erectile dysfunction-associated changes. Urology2011; 78: 970.e1-8.
2. Gondre M, Christ GJ. Endothelin-1-induced alterations in phenylephrine-inducedcontractile responses are largely additive in physiologically diverse rabbit vasculature.J Pharmacol Exp Ther 1998; 286: 635-642. (Pubitemid 29003449)
3. Italiano G, Calabro A, Spini S, Ragazzi E, Pagano F. Functional response ofcavernosal tissue to distension. Urol Res 1998; 26: 39-44. (Pubitemid 28218181)
4. Hurt KJ, Musicki B, Palese MA, Crone JK, Becker RE, Moriarity JL et al. Aktdependentphosphorylation of endothelial nitric-oxide synthase mediates penileerection. Proc Natl Acad Sci USA 2002; 99: 4061-4066. (Pubitemid 34252229)
5. Schuster A, Oishi H, Beny JL, Stergiopulos N, Meister JJ. Simultaneous arterialcalcium dynamics and diameter measurements: application to myoendothelialcommunication. Am J Physiol Heart Circ Physiol 2001; 280: H1088-H1096.
6. Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology, andpharmacology. Pharmacol Rev 1991; 43: 109-142.
7. Niwa K, Kado T, Sakai J, Karino T. The effects of a shear flow on the uptake of LDLand acetylated LDL by an EC monoculture and an EC-SMC coculture. Ann BiomedEng 2004; 32: 537-543.
8. Truskey GA. Endothelial cell vascular smooth muscle cell co-culture assay for highthroughput screening assays for discovery of anti-angiogenesis agents and othertherapeutic molecules. Int J High Throughput Screen 2010; 2010: 171-181.
9. Powell RJ, Cronenwett JL, Fillinger MF, Wagner RJ, Sampson LN. Endothelialcell modulation of smooth muscle cell morphology and organizational growthpattern. Ann Vasc Surg 1996; 10: 4-10. (Pubitemid 26112515)
10. Lin CS, Lin G, Wang Z, Maddah SA, Lue TF. Upregulation of monocyte chemoattractantprotein 1 and effects of transforming growth factor-beta 1 inPeyronie's disease. Biochem Biophys Res Commun 2002; 295: 1014-1019.
11. Ning H, Lin G, Lue TF, Lin CS. Effects of estrogen, raloxifene, and levormeloxifeneon the expression of Rho-kinase signaling molecules in urethral smooth musclecells. Urology 2011; 76: 1517.e6-11.
12. van Beijnum JR, Rousch M, Castermans K, van der Linden E, Griffioen AW. Isolationof endothelial cells from fresh tissues. Nat Protoc 2008; 3: 1085-1091. (Pubitemid 351818695)
13. Evensen L, Micklem DR, Blois A, Berge SV, Aarsaether N, Littlewood-Evans A et al.Mural cell associated VEGF is required for organotypic vessel formation. PLoS ONE2009; 4: e5798-e5808.
14. Thorve VS, Kshirsagar AD, Vyawahare NS, Joshi VS, Ingale KG, Mohite RJ. Diabetesinducederectile dysfunction: epidemiology, pathophysiology and management.J Diabetes Complications 2011; 25: 129-136.
15. Jin HR, Kim WJ, Song JS, Piao S, Tumurbaatar M, Shin SH et al. Intracavernousdelivery of synthetic angiopoietin-1 protein as a novel therapeutic strategyfor erectile dysfunction in the type II diabetic db/db mouse. J Sex Med 2010; 7:3635-3646.
16. Podlasek CA, Zelner DJ, Harris JD, Meroz CL, Tang Y, McKenna KE et al. AlteredSonic hedgehog signaling is associated with morphological abnormalities in thepenis of the BB/WOR diabetic rat. Biol Reprod 2003; 69: 816-827. (Pubitemid 37064609)
17. Albersen M, Lin G, Fandel TM, Zhang H, Qiu X, Lin CS et al. Functional, metabolic,and morphologic characteristics of a novel rat model of type 2 diabetes-associatederectile dysfunction. Urology 2011; 78: 476.e1-8.
18. Cagliero E, Maiello M, Boeri D, Roy S, Lorenzi M. Increased expression of basementmembrane components in human endothelial cells cultured in high glucose.J Clin Invest 1988; 82: 735-738.
19. Hayden MR, Sowers JR, Tyagi SC. The central role of vascular extracellular matrixand basement membrane remodeling in metabolic syndrome and type 2 diabetes:the matrix preloaded. Cardiovasc Diabetol 2005; 4: 9-28.
20. Britland ST, Young RJ, Sharma AK, Clarke BF. Relationship of endoneurial capillaryabnormalities to type and severity of diabetic polyneuropathy. Diabetes 1990; 39:909-913. (Pubitemid 20233722)