Refolding from denatured inclusion bodies, purification to homogeneity and simplified assay of MGDG synthases from land plants

Protein Expression and Purification

Volumn 31, Issue 1, 2003, Pages 79-87

  Nishiyama, Yoshitaka ^a,c   Hardré Liénard, Hélène ^a   Miras, Stéphane ^a   Miège, Christine ^a,d   Block, Maryse A ^a   Revah, Frédéric ^b   Joyard, Jacques ^a   Maréchal, Eric ^a  

^a CNRS   (France)

^b CEREP   (France)

^c EHIME UNIVERSITY   (Japan)

^d UNIVERSITÉ DE LYON   (France)

Author keywords

Anti parasitic drug; Galactolipid; Herbicide; High throughput screening; Membrane protein; MGDG synthase; Refolding

Indexed keywords

BACTERIA (MICROORGANISMS); EMBRYOPHYTA; ESCHERICHIA COLI; PROTISTA; SPINACIA OLERACEA;

EID: 0141539506     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(03)00158-X     Document Type: Article

References (14)

1. M.A. Block, E. Maréchal, J. Joyard, in: E.M. Aro, B. Andersson (Eds.), Advances in Photosynthesis and Respiration, Regulation of Photosynthesis, vol. 11, Kluwer Academic, Dordrecht, The Netherlands, 2001, pp. 195-218.
2. E. Maréchal, N. Azzouz, C. Santos de Macedo, M.A. Block, J.E. Feagin, R.T. Schwarz, J. Joyard, Synthesis of chloroplast galactolipids in Apicomplexan parasites, Eukaryotic Cell 1 (2002) 653-656.
3. E. Maréchal, M.F. Cesbron-Delauw, The apicoplast: a new member of the plastid family, Trends Plant Sci. 6 (2001) 200-205.
4. E. Maréchal, C. Miège, M.A. Block, R. Douce. J. Joyard, The catalytic site of MGDG synthase from spinach chloroplast envelope membranes: biochemical analysis of the structure and of the metal content, J. Biol. Chem. 270 (1995) 5714-5722.
5. M. Shimojima, H. Ohta, A. Iwamatsu, T. Masuda, Y. Shioi, K.-I. Takamiya, Cloning of the gene for monogalactosyldiacylglycerol synthase and its evolutionary origin, Proc. Natl. Acad. Sci. USA 94 (1997) 333-337.
6. C. Miège, E. Maréchal, M. Shimojima, K. Awai, M.A. Block, H. Ohta, K.-I. Takamiya, R. Douce, J. Joyard, Biochemical and topological properties of Type A MGDG synthase, a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG, Eur. J. Biochem. 265 (1999) 990-1001.
7. K. Awai, E. Maréchal, M.A. Block, D. Brun, T. Masuda, H. Shimada, K.-I. Takamiya, H. Ohta, J. Joyard, Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana, Proc. Natl. Acad. Sci. USA 98 (2001) 10960-10965.
8. E.G. Bligh, W.J. Dyer, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol. 37 (1959) 911-917.
9. M. Gad, K. Awai, M. Shimojima, Y. Yamaryo, H. Shimada, T. Masuda, K.-I. Takamiya, A. Ikai, H. Ohta, Accumulation of plant galactolipid affects cell morphology of Escherichia coli, Biochem. Biophys. Res. Commun. 286 (2001) 114-118.
10. A.G. Rietveld, V.V. Chupin, M.C. Koorengevel, H.L. Wienk, W. Dowhan, B. de Kruijff, Regulation of lipid polymorphism is essential for the viability of phosphatidylethanolamine-deficient Escherichia coli cells, J. Biol. Chem. 269 (1994) 28670-28675.
11. P. Jorasch, D.C. Warnecke, B. Lindner, U. Zahringer, E. Heinz, Novel processive and nonprocessive glycosyltransferases from Staphylococcus aureus and Arabidopsis thaliana synthesize glycoglycerolipids, glycophospholipids, glycosphingolipids and glycosylsterols, Eur. J. Biochem. 267 (2000) 3770-3783.
12. P. Dormann, I. Balbo, C. Benning, Arabidopsis galactolipid biosynthesis and lipid trafficking mediated by DGD1, Science 284 (1999) 2181-2184.
13. R. Rainer, L. Hauke, In vitro folding of inclusion body proteins, FASEB J. 50 (1996) 49-56.
14. J.U. Zhang, T.D.Y. Chung, K.R. Oldenburg, Confirmation of primary active substances from high throughput screening of chemical and biological populations: a statistical approach and practical considerations, J. Comb. Chem. 2 (2000) 258-265.