Five new genes are important for common polysaccharide antigen biosynthesis in Pseudomonas aeruginosa

mBio

Volumn 4, Issue 1, 2013, Pages

  Hao, Youai ^a   King, Jerry D ^a,b   Huszczynski, Steven ^a   Kocíncová, Dana ^a   Lam, Joseph S ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b University of Bath   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BETA GALACTOSIDASE; COMMON POLYSACCHARIDE ANTIGEN; UNCLASSIFIED DRUG; LIPOPOLYSACCHARIDE;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CONTROLLED STUDY; DELETION MUTANT; GENE CLUSTER; GENE FUNCTION; GENE LOCUS; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; MUTATIONAL ANALYSIS; NONHUMAN; OPERON; PA5455 GENE; PA5456 GENE; PA5457 GENE; PA5458 GENE; PA5459 GENE; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; START CODON; TRANSCRIPTION INITIATION SITE; WESTERN BLOTTING; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MULTIGENE FAMILY;

ANTIGENS, BACTERIAL; BIOSYNTHETIC PATHWAYS; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; LIPOPOLYSACCHARIDES; MULTIGENE FAMILY; OPERON; PSEUDOMONAS AERUGINOSA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

MLCS; MLOWN;

EID: 84874623118     PISSN: 21612129     EISSN: 21507511     Source Type: Journal    
DOI: 10.1128/mBio.00631-12     Document Type: Article

References (31)

1. Greenfield LK, Whitfield C. 2012. Synthesis of lipopolysaccharide O-antigens by ABC transporter-dependent pathways. Carbohydr. Res. 356:12-24.
2. Burns SM, Hull SI. 1998. Comparison of loss of serum resistance by defined lipopolysaccharide mutants and an acapsular mutant of uropathogenic Escherichia coli O75 K5. Infect. Immun. 66:4244-4253.
3. Kos V, Cuthbertson L, Whitfield C. 2009. The Klebsiella pneumoniae O2a antigen defines a second mechanism for O antigen ATP-binding cassette transporters. J. Biol. Chem. 284:2947-2956.
4. Clarke BR, Cuthbertson L, Whitfield C. 2004. Nonreducing terminal modifications determine the chain length of polymannose O antigens of Escherichia coli and couple chain termination to polymer export via an ATP-binding cassette transporter. J. Biol. Chem. 279:35709-35718.
5. Cuthbertson L, Kimber MS, Whitfield C. 2007. Substrate binding by a bacterial ABC transporter involved in polysaccharide export. Proc. Natl. Acad. Sci. U. S. A. 104:19529-19534.
6. Cryz SJ, Jr, Pitt TL, Fürer E, Germanier R. 1984. Role of lipopolysaccharide in virulence of Pseudomonas aeruginosa. Infect. Immun. 44: 508-513.
7. Lam JS, Taylor VL, Islam ST, Hao Y, Kocíncová D. 2011. Genetic and functional diversity of Pseudomonas aeruginosa lipopolysaccharide. Front. Microbiol. 2:118. http://dx.doi.org/10.3389/fmicb.2011.00118.
8. King JD, Kocíncová D, Westman EL, Lam JS. 2009. Lipopolysaccharide biosynthesis in Pseudomonas aeruginosa. Innate Immun. 15:261-312.
9. Lam MY, McGroarty EJ, Kropinski AM, MacDonald LA, Pedersen SS, Hoiby N, Lam JS. 1989. Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa. J. Clin. Microbiol. 27:962-967.
10. Matewish M. 2004. The functional role of lipopolysaccharide in the cell envelope and surface proteins of Pseudomonas aeruginosa. Ph.D. thesis. University of Guelph, Guelph, Canada.
11. Arsenault TL, et al. 1991. Structural studies on the polysaccharide portion of "A-band" lipopolysaccharide from a mutant (AK14O1) of Pseudomonas aeruginosa PAO1. Can. J. Chem. 69:1273-1280.
12. Choudhury B, Carlson RW, Goldberg JB. 2005. The structure of the lipopolysaccharide from a galU mutant of Pseudomonas aeruginosa serogroup-O11. Carbohydr. Res. 340:2761-2772.
13. Yokota S, Kaya S, Sawada S, Kawamura T, Araki Y, Ito E. 1987. Characterization of a polysaccharide component of lipopolysaccharide from Pseudomonas aeruginosa IID 1008 (ATCC 27584) as D-rhamnan. Eur. J. Biochem. 167:203-209.
14. Rocchetta HL, Burrows LL, Pacan JC, Lam JS. 1998. Three rhamnosyltransferases responsible for assembly of the A-band D-rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for the initiation of both A-band and B-band lipopolysaccharide synthesis. Mol. Microbiol. 28:1103-1119.
15. Rocchetta HL, Lam JS. 1997. Identification and functional characterization of an ABC transport system involved in polysaccharide export of A-band lipopolysaccharide in Pseudomonas aeruginosa. J. Bacteriol. 179: 4713-4724.
16. Rocchetta HL, Pacan JC, Lam JS. 1998. Synthesis of the A-band polysaccharide sugar D-rhamnose requires Rmd and WbpW: identification of multiple AlgA homologues, WbpW and ORF488, in Pseudomonas aeruginosa. Mol. Microbiol. 29:1419-1434.
17. Fenton MS, Lee SJ, Gralla JD. 2000. Escherichia coli promoter opening and-10 recognition: mutational analysis of sigma70. EMBO J. 19: 1130-1137.
18. Tanaka K, Takahashi H. 1991. Cloning and analysis of the gene (rpoDA) for the principal sigma factor of Pseudomonas aeruginosa. Biochim. Biophys. Acta 1089:113-119.
19. Potvin E, Sanschagrin F, Levesque RC. 2008. Sigma factors in Pseudomonas aeruginosa. FEMS Microbiol. Rev. 32:38-55.
20. Clarke BR, Richards MR, Greenfield LK, Hou D, Lowary TL, Whitfield C. 2011. In vitro reconstruction of the chain termination reaction in biosynthesis of the Escherichia coli O9a O-polysaccharide: the chain-length regulator, WbdD, catalyzes the addition of methyl phosphate to the nonreducing terminus of the growing glycan. J. Biol. Chem. 286: 41391-41401.
21. Clarke BR, Greenfield LK, Bouwman C, Whitfield C. 2009. Coordination of polymerization, chain termination, and export in assembly of the Escherichia coli lipopolysaccharide O9a antigen in an ATP-binding cassette transporter-dependent pathway. J. Biol. Chem. 284:30662-30672.
22. Ostler S. 2009. Regulation of two genes, migA and wapR, involved in core LPS biosynthesis in Pseudomonas aeruginosa. M.S. thesis. University of Guelph, Guelph, Canada.
23. Miller J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, New York, NY.
24. Choi KH, Schweizer HP. 2005. An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMCMicrobiol. 5:30.
25. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:77-86.
26. Qiu D, Damron FH, Mima T, Schweizer HP, Yu HD. 2008. PBAD based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria. Appl. Environ. Microbiol. 74:7422-7426.
27. Choi KH, Kumar A, Schweizer HP. 2006. A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J. Microbiol. Methods 64:391-397.
28. Hitchcock PJ, Brown TM. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
29. Fomsgaard A, Freudenberg MA, Galanos C. 1990. Modification of the silver staining technique to detect lipopolysaccharide in polyacrylamide gels. J. Clin. Microbiol. 28:2627-2631.
30. de Kievit TR, Dasgupta T, Schweizer H, Lam JS. 1995. Molecular cloning and characterization of the rfc gene of Pseudomonas aeruginosa (serotype O5). Mol. Microbiol. 16:565-574.
31. Blake MS, Johnston KH, Russell-Jones GJ, Gotschlich EC. 1984. A rapid, sensitive method for detection of alkaline phosphatase-conjugated antiantibody on Western blots. Anal. Biochem. 136:175-179.