Fluorescence imaging of flowing cells using a temporally coded excitation

Optics Express

Volumn 21, Issue 4, 2013, Pages 5164-5170

  Gorthi, Sai Siva ^a   Schaak, Diane ^a   Schonbrun, Ethan ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; FLUORESCENCE;

CELLULAR ORGANELLES; CODED EXCITATION; DIFFRACTION LIMITED; EXCITATION PULSE; EXPOSURE-TIME; FLUID ENVIRONMENT; FLUORESCENCE IMAGING; IMAGING FLUORESCENCES; LEUKEMIA CELLS; MOTION BLUR; PSEUDO RANDOM; VASCULATURE;

CELLS;

CELL SEPARATION; DEVICE FAILURE ANALYSIS; DEVICES; EQUIPMENT DESIGN; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; ILLUMINATION; IMAGE ENHANCEMENT; LEUKEMIA; PATHOLOGY; PROCEDURES; TUMOR CELL LINE; ARTICLE; EQUIPMENT; EQUIPMENT FAILURE; METHODOLOGY;

CELL LINE, TUMOR; CELL SEPARATION; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FLOW CYTOMETRY; HUMANS; IMAGE ENHANCEMENT; LEUKEMIA; LIGHTING; MICROSCOPY, FLUORESCENCE;

EID: 84874523135     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.21.005164     Document Type: Article

References (16)

1. R. Yuste, "Fluorescence microscopy today," Nat. Methods 2(12), 902-904 (2005).
2. B. Huang, H. Babcock, and X. Zhuang, "Breaking the diffraction barrier: super-resolution imaging of cells," Cell 143(7), 1047-1058 (2010).
3. L. A. Sklar, Flow Cytometry for Biotechnology (Oxford, 2005).
4. T. R. Jones, A. E. Carpenter, M. R. Lamprecht, J. Moffat, S. J. Silver, J. K. Grenier, A. B. Castoreno, U. S. Eggert, D. E. Root, P. Golland, and D. M. Sabatini, "Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning," Proc. Natl. Acad. Sci. U.S.A. 106(6), 1826-1831 (2009).
5. D. A. Basiji and W. E. Ortyn, Imaging and analyzing parameters of small moving objects such as cells. Amnis Corporation, assignee. US Patent 6211955, 2000-03-29 (2001).
6. R. D. Kamm, "Cellular fluid mechanics," Annu. Rev. Fluid Mech. 34(1), 211-232 (2002).
7. S. Suresh, "Biomechanics and biophysics of cancer cells," Acta Biomater. 3(4), 413-438 (2007).
8. J. W. Lichtman and J. A. Conchello, "Fluorescence Microscopy," Nat. Methods 2(12), 910-919 (2005).
9. J. Vermot, S. E. Fraser, and M. Liebling, "Fast fluorescence microscopy for imaging the dynamics of embryonic development," HFSP J 2(3), 143-155 (2008).
10. B. K. McKenna, J. G. Evans, M. C. Cheung, and D. J. Ehrlich, "A parallel microfluidic flow cytometer for highcontent screening," Nat. Methods 8(5), 401-403 (2011).
11. E. Schonbrun, A. R. Abate, P. E. Steinvurzel, D. A. Weitz, and K. B. Crozier, "High-throughput fluorescence detection using an integrated zone-plate array," Lab Chip 10(7), 852-856 (2010).
12. E. Schonbrun, S. S. Gorthi, and D. Schaak, "Microfabricated multiple field of view imaging flow cytometry," Lab Chip 12(2), 268-273 (2011).
13. R. Raskar, A. Agrawal, and J. Tumblin, "Coded exposure photography: motion deblurring using fluttered shutter," ACM SIGGRAPG 25(3), 795-804 (2006).
14. P. Kiesel, M. Bassler, M. Beck, and N. Johnson, "Spatially modulated fluorescence emission from moving particles," Appl. Phys. Lett. 94(4), 041107 (2009).
15. R. C. Gonzalez, R. E. Woods, and S. L. Eddins, Digital Image Processing Using Matlab (Gatemark, 2009).
16. R. A. Saleem, B. Knoblach, F. D. Mast, J. J. Smith, J. Boyle, C. M. Dobson, R. Long-O'Donnell, R. A. Rachubinski, and J. D. Aitchison, "Genome-wide analysis of signaling networks regulating fatty acid-induced gene expression and organelle biogenesis," J. Cell Biol. 181(2), 281-292 (2008).