A quantitative allele-specific PCR test for the BRAF V600E mutation using a single heterozygous control plasmid for quantitation: A model for qPCR testing without standard curves

Journal of Molecular Diagnostics

Volumn 15, Issue 2, 2013, Pages 248-254

  Szankasi, Philippe ^a   Reading, N Scott ^a   Vaughn, Cecily P ^a   Prchal, Josef T ^b   Bahler, David W ^b   Kelley, Todd W ^b  

^a ARUP LABORATORIES   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

B RAF KINASE;

ALLELE; ARTICLE; CALIBRATOR; CONCENTRATION (PARAMETERS); DNA EXTRACTION; FLOW CYTOMETRY; GENE FREQUENCY; GENE MUTATION; HAIRY CELL LEUKEMIA; HUMAN; HUMAN CELL; HUMAN TISSUE; LEUKEMIA; LIMIT OF QUANTITATION; PYROSEQUENCING; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; WILD TYPE;

ALLELES; CELL LINE, TUMOR; HUMANS; MUTATION; PLASMIDS; PROTO-ONCOGENE PROTEINS B-RAF; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY;

EID: 84874521439     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2012.11.005     Document Type: Article

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