Epimedium-derived flavonoids modulate the balance between osteogenic differentiation and adipogenic differentiation in bone marrow stromal cells of ovariectomized rats via Wnt/β-catenin signal pathway activation

Chinese Journal of Integrative Medicine

Volumn 18, Issue 12, 2012, Pages 909-917

  Xu, Ying Xing ^a,b   Wu, Cheng Liang ^a   Wu, Yan ^a   Tong, Pei Jian ^c   Jin, Hong Ting ^a   Yu, Nan Ze ^a   Xiao, Lu Wei ^a  

^a ZHEJIANG CHINESE MEDICAL UNIVERSITY   (China)

^b Chinese Medicine Hospital   (China)

^c Chinese Medicine Hospital   (China)

Author keywords

adipogenesis; bone marrow stromal cells; cell differentiation; epimedium derived flavonoids; osteogenesis; osteoporosis; wnt catenin signal pathway

Indexed keywords

ALKALINE PHOSPHATASE; BETA CATENIN; CCAAT ENHANCER BINDING PROTEIN ALPHA; COLLAGEN TYPE 1; DICKKOPF 1 PROTEIN; FLAVONOID; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 5; MESSENGER RNA; OSTEOCALCIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; T CELL FACTOR PROTEIN; TRANSCRIPTION FACTOR RUNX2; WNT PROTEIN;

ADIPOCYTE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIMEDIUM; FEMALE; GENE EXPRESSION; NONHUMAN; OSTEOBLAST; OVARIECTOMY; POSTMENOPAUSE OSTEOPOROSIS; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; STROMA CELL; UPREGULATION; WNT SIGNALING PATHWAY;

ADIPOSE TISSUE; ANIMALS; BASE SEQUENCE; BETA CATENIN; BONE AND BONES; CELL DIFFERENTIATION; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPIMEDIUM; FEMALE; FLAVONOIDS; FLOW CYTOMETRY; MESENCHYMAL STROMAL CELLS; RATS; REAL-TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WNT PROTEINS;

EID: 84874483015     PISSN: 16720415     EISSN: 19930402     Source Type: Journal    
DOI: 10.1007/s11655-012-1294-2     Document Type: Article

References (21)

1. Meuniher P, Aaron J, Edouard C, Vignon G. Osteoporosis and the replacement of cell populations of the marrow by adipose tissue. A quantitative study of 84 iliac bone biopsies. Clin Orthop Relat Res 1971;80:147-154.
2. Rodriguez JP, Garat S, Gajardo H, Pino AM, Seitz G. Abnormal osteogenesis in osteoporotic patients is reflected by altered mesenchymal stem cells dynamics. J Cell Biochem 1999;75:414-423.
3. Rodriguez JP, Montecinos L, Rios S, Reyes P, Martinez J. Mesenchymal stem cells from osteoporotic patients produce a type I collagen-deficient extracellular matrix favoring adipogenic differentiation. J Cell Biochem 2000;79:557-565.
4. Qiu W, Andersen TE, Bollerslev J, Mandrup S, Abdallah BM, Kassem M. Patients with high bone mass phenotype exhibit enhanced osteoblast differentiation and inhibition of adipogenesis of human mesenchymal stem cells. J Bone Miner Res 2007;22:1720-1731.
5. Bennett CN, Longo KA, Wright WS, Suva LJ, Lane TF, Hankenson KD, et al. Regulation of osteoblastogenesis and bone mass by Wnt10b. Proc Natl Acad Sci U S A 2005;102:3324-3329.
6. Zhang G, Qin L, Shi Y. Epimedium-derived phytoestrogen flavonoids exert beneficial effect on preventing bone loss in late postmenopausal women: a 24-month randomized, double-blind and placebo-controlled trial. J Bone Miner Res 2007;22:1072-1079.
7. Peng S, Zhang G, He Y, Wang X, Leung P, Leung K, et al. Epimedium-derived flavonoids promote osteoblastogenesis and suppress adipogenesis in bone marrow stromal cells while exerting an anabolic effect on osteoporotic bone. Bone 2009;45:534-544.
8. Li D, Li L, Wu J. Adipogenic potential of bone marrow mesenchymal stem cells derived from ovariectomized rats. J Biomed Eng (Chin) 2007;24:641-645.
9. Li DJ, Ge DX, Wu WC, Wu J, Li L. Osteogenic potential of bone marrow mesenchymal stem cells from ovariectomied osteoporotic rat. J Sichuan Univ (Med Sci, Chin) 2005;36:318-321.
10. Gaur T, Lengner CJ, Hovhannisyan H, Bhat RA, Bodine PVN, Komm BS, et al. Canonical WNT signaling promotes osteogenesis by directly stimulating runx2 gene expression. J Biol Chem 2005;280:33132-33140.
11. Zhang Y, Deng X, Scheller EL, Kwon TG, Lahann J, Franceschi RT, et al. The effects of Runx 2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitro. Biomaterials 2010;31:3231-3236.
12. Wang FS, Ko JY, Yeh DW, Ke HC, Wu HL. Modulation of Dickkopf-1 attenuates glucocorticoid induction of osteoblast apoptosis, adipocytic differentiation, and bone mass loss. Endocrinology 2008;149:1793-1801.
13. Gustafson B, Smith U. Cytokines promote Wnt signaling and inflammation and impair the normal differentiation and lipid accumulation in 3T3-L1 preadipocytes. J Biol Chem 2006;281:9507-9516.
14. Franceschi RT, Xiao G. Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways. J Cell Biochem 2003;88:446-454.
15. Karsenty G, Ducy P, Starbuck M, Priemel M, Shen J, Geoffroy V, et al. Cbfa1 as a regulator of osteoblast differentiation and function. Bone 1999;25:107-108.
16. Lin TH, Yang RS, Tang CH, Lin CP, Fu WM. PPAR gamma inhibits osteogenesis via the down-regulation of the expression of COX-2 and iNOS in rats. Bone 2007;41:562-574.
17. Schadinger SE, Bucher NL, Schreiber BM, Farmer SR. PPAR gamma 2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes. Am J Physiol Endocrinol Metab 2005;288:E1195-E1205.
18. Jiang MS, Lane MD. Sequential repression and activation of the CCAAT enhancer-binding protein-α (C/EBPα) gene during adipogenesis. Proc Natl Acad Sci U S A 2000;7:12519-12523.
19. Valeria P. The Role of C/EBP isoforms in the control of inflammatory and native immunity functions. J Biol Chem 1998;273:29279-29282.
20. Darlington GJ, Ross SE, MacDougald OA. The role of C/EBP genes in adipocyte differentiation. J Biol Chem 1998;273:30057-30060.
21. Ai M, Holmen SL, Van HW, Williams BO, Warman ML. Reduced affinity to and inhibition by DKK1 form a common mechanism by which high bone mass-associated missense mutations in LRP5 affect canonical Wnt signaling. Mol Cell Biol 2005;25:4946-4955.