Combining COLD-PCR and high-resolution melt analysis for rapid detection of low-level, rifampin-resistant mutations in Mycobacterium tuberculosis

Journal of Microbiological Methods

Volumn 93, Issue 1, 2013, Pages 32-36

  Pang, Yu ^a   Liu, Guan ^b   Wang, Yufeng ^a   Zheng, Suhua ^b   Zhao, Yan Lin ^a  

^a CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

COLD PCR; HRM; Tuberculosis

Indexed keywords

RIFAMPICIN; RNA POLYMERASE BETA SUBUNIT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CO AMPLIFICATION LOWER DENATURATION TEMPERATURE POLYMERASE CHAIN REACTION; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; GENE MUTATION; HIGH RESOLUTION MELTING ANALYSIS; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MEASUREMENT ACCURACY; MUTATIONAL ANALYSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; RNA POLYMERASE BETA SUBUNIT GENE; SENSITIVITY ANALYSIS;

ANTITUBERCULAR AGENTS; DNA, BACTERIAL; DNA-DIRECTED RNA POLYMERASES; DRUG RESISTANCE, BACTERIAL; MUTATION; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; RIFAMPIN; SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE;

EID: 84874445496     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.01.008     Document Type: Article

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