Broad-substrate screen as a tool to identify substrates for bacterial Gcn5-related N-acetyltransferases with unknown substrate specificity

Protein Science

Volumn 22, Issue 2, 2013, Pages 222-230

  Kuhn, Misty L ^a   Majorek, Karolina A ^b   Minor, Wladek ^b   Anderson, Wayne F ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Acetylation of antibiotics; Broadsubstrate screen; Ellman's reagent; Gcn5 related acetyltransferase; GNAT; N acetyltransferase

Indexed keywords

ACYLTRANSFERASE; ADENOSINE; ALLANTOIN; AMINONUCLEOSIDE; AMPICILLIN; APRAMYCIN; BACITRACIN; CATECHOLAMINE; CEFALEXIN; CEPHALOSPORIN; CHLORAMPHENICOL; COLISTIN; CYSTEINE; CYTIDINE; FOLIC ACID; GENERAL CONTROL OF NUTRITION RELATED N ACETYLTRANSFERASE; GUANOSINE; KANAMYCIN B; POLYAMINE; POLYMYXIN B; PUROMYCIN; PYRIDOXAMINE; STREPTOMYCIN; SULFACETAMIDE; SULFONAMIDE; THIAMINE; TOBRAMYCIN; UNCLASSIFIED DRUG; UNINDEXED DRUG; UREA; VITAMIN;

ACETYLATION; ACIDITY; ARTICLE; ENZYME ACTIVITY; ENZYME SPECIFICITY; HIGH TEMPERATURE; PH; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS;

ACETYLTRANSFERASES; AGROBACTERIUM TUMEFACIENS; CLOSTRIDIUM DIFFICILE; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS; SUBSTRATE SPECIFICITY; VIBRIO CHOLERAE;

EID: 84874049400     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2199     Document Type: Article

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