Construction of a Ca2+-gated artificial channel by fusing alamethicin with a calmodulin-derived extramembrane segment

Bioconjugate Chemistry

Volumn 24, Issue 2, 2013, Pages 188-195

  Noshiro, Daisuke ^a   Sonomura, Kazuhiro ^a   Yu, Hao Hsin ^a   Imanishi, Miki ^a   Asami, Koji ^a   Futaki, Shiroh ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

ALAMETHICIN; C-TERMINAL DOMAINS; CA 2+; CHANNEL CONDUCTANCE; CHANNEL CURRENTS; CHANNEL PROTEINS; DISCRETE CHANNEL; EXTRAMEMBRANES; FUSION PROTEINS; NATIVE CHEMICAL LIGATION;

CALMODULIN;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ALAMETHICIN; CALCIUM CHANNEL; CALMODULIN; MAGNESIUM;

ARTICLE; CALCIUM CONDUCTANCE; CHEMICAL REACTION; CHEMICAL STRUCTURE; FLUORESCENCE; HYDROPHOBICITY; PH; STIMULATION;

EID: 84874043475     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc300468x     Document Type: Article

References (41)

1. Majd, S., Yusko, E. C., Billeh, Y. N., Macrae, M. X., Yang, J., and Mayer, M. (2010) Applications of biological pores in nanomedicine, sensing, and nanoelectronics Curr. Opin. Biotechnol. 21, 439-76
2. Bayley, H. and Jayasinghe, L. (2004) Functional engineered channels and pores (Review) Mol. Membr. Biol. 21, 209-20
3. Matile, S., Jentzsch, A. V., Montenegro, J., and Fin, A. (2011) Recent synthetic transport systems Chem. Soc. Rev. 40, 2453-74
4. Maglia, G., Heron, A. J., Stoddart, D., Japrung, D., and Bayley, H. (2010) Analysis of single nucleic acid molecules with protein nanopores Methods Enzymol. 475, 591-623
5. Futaki, S. (1998) Peptide ion channels: design and creation of function Biopolymers 47, 75-81
6. Futaki, S. and Asami, K. (2007) Ligand-induced extramembrane conformation switch controlling alamethicin assembly and the channel current Chem. Biodivers. 4, 1313-22
7. Berridge, M. J., Lipp, P., and Bootman, M. D. (2000) The versatility and universality of calcium signalling Nat. Rev. Mol. Cell Biol. 1, 11-21
8. Berridge, M. J., Bootman, M. D., and Roderick, H. L. (2003) Calcium signalling: dynamics, homeostasis and remodelling Nat. Rev. Mol. Cell Biol. 4, 517-29
9. Zhang, Y., Ruder, W. C., and LeDuc, P. R. (2008) Artificial cells: building bioinspired systems using small-scale biology Trends Biotechnol. 26, 14-20
10. Leitgeb, B., Szekeres, A., Manczinger, L., Vágvölgyi, C., and Kredics, L. (2007) The history of alamethicin: a review of the most extensively studied peptaibol Chem. Biodivers. 4, 1027-51
11. Noshiro, D., Asami, K., and Futaki, S. (2010) Metal-assisted channel stabilization: disposition of a single histidine on the N-terminus of alamethicin yields channels with extraordinarily long lifetimes Biophys. J. 98, 1801-8
12. Woolley, G. A., Epand, R. M., Kerr, I. D., Sansom, M. S. P., and Wallace, B. A. (1994) Alamethicin pyromellitate: an ion-activated channel-forming peptide Biochemistry 33, 6850-8
13. Woolley, G. A. and Lougheed, T. (2003) Modeling ion channel regulation Curr. Opin. Chem. Biol. 7, 710-4
14. Futaki, S., Fukuda, M., Omote, M., Yamauchi, K., Yagami, T., Niwa, M., and Sugiura, Y. (2001) Alamethicin-leucine zipper hybrid peptide: a prototype for the design of artificial receptors and ion channels J. Am. Chem. Soc. 123, 12127-34
15. Kiwada, T., Sonomura, K., Sugiura, Y., Asami, K., and Futaki, S. (2006) Transmission of extramembrane conformational change into current: construction of metal-gated ion channel J. Am. Chem. Soc. 128, 6010-1
16. Jiang, Y., Lee, A., Chen, J., Cadene, M., Chait, B. T., and MacKinnon, R. (2002) Crystal structure and mechanism of a calcium-gated potassium channel Nature 417, 515-22
17. Futaki, S., Sogawa, K., Maruyama, J., Asahara, T., Niwa, M., and Hojo, H. (1997) Preparation of peptide thioesters using Fmoc-solid-phase peptide synthesis and its application to the construction of a template-assembled synthetic protein (TASP) Tetrahedron Lett. 38, 6237-40
18. Xu, M. Q. and Evans, T. C., Jr. (2001) Intein-mediated ligation and cyclization of expressed proteins Methods 24, 257-77
19. Asami, K., Okazaki, T., Nagai, Y., and Nagaoka, Y. (2002) Modifications of alamethicin ion channels by substitution of Glu-7 for Gln-7 Biophys. J. 83, 219-28
20. 2+-induced conformational changes in calmodulin Biophys. Chem. 35, 75-84
21. Zhang, Y., Futaki, S., Kiwada, T., and Sugiura, Y. (2002) Detection of protein-ligand interaction on the membranes using C-terminus biotin-tagged alamethicin Bioorg. Med. Chem. 10, 2635-9
22. Babu, Y. S., Sack, J. S., Greenhough, T. J., Bugg, C. E., Means, A. R., and Cook, W. J. (1985) Three-dimensional structure of calmodulin Nature 315, 37-40
23. 2+ based on green fluorescent proteins and calmodulin Nature 388, 882-7
24. 2+ sensor Nat. Methods 5, 797-804
25. Johnson, J. D. and Wittenauer, L. A. (1983) A fluorescent calmodulin that reports the binding of hydrophobic inhibitory ligands Biochem. J. 211, 473-9
26. Wriggers, W., Mehler, E., Pitici, F., Weinstein, H., and Schulten, K. (1998) Structure and dynamics of calmodulin in solution Biophys. J. 74, 1622-39
27. Slaughter, B. D., Unruh, J. R., Allen, M. W., Bieber Urbauer, R. J., and Johnson, C. K. (2005) Conformational substates of calmodulin revealed by single-pair fluorescence resonance energy transfer: influence of solution conditions and oxidative modification Biochemistry 44, 3694-707
28. Isvoran, A., Craescu, C. T., and Alexov, E. (2007) Electrostatic control of the overall shape of calmodulin: numerical calculations Eur. Biophys. J. 36, 225-37
29. Barth, A., Martin, S. R., and Bayley, P. M. (1998) Specificity and symmetry in the interaction of calmodulin domains with the skeletal muscle myosin light chain kinase target sequence J. Biol. Chem. 273, 2174-83
30. 2+ on the secondary and tertiary structure of bovine testis calmodulin. A circular-dichroism study Biochem. J. 238, 485-90
31. Linse, S., Helmersson, A., and Forsén, S. (1991) Calcium binding to calmodulin and its globular domains J. Biol. Chem. 266, 8050-9
32. Dawson, P. E., Muir, T. W., Clark-Lewis, I., and Kent, S. B. H. (1994) Synthesis of proteins by native chemical ligation Science 266, 776-9
33. Finn, B. E., Evenäs, J., Drakenberg, T., Waltho, J. P., Thulin, E., and Forsén, S. (1995) Calcium-induced structural changes and domain autonomy in calmodulin Nat. Struct. Biol. 2, 777-83
34. Woolley, G. A. (2007) Channel-forming activity of alamethicin: effects of covalent tethering Chem. Biodivers. 4, 1323-37
35. Okazaki, T., Sakoh, M., Nagaoka, Y., and Asami, K. (2003) Ion channels of alamethicin dimer N-terminally linked by disulfide bond Biophys. J. 85, 267-73
36. 28k Biochemistry 39, 6864-73
37. Schauer-Vukasinovic, V., Cullen, L., and Daunert, S. (1997) Rational design of a calcium sensing system based on induced conformational changes of calmodulin J. Am. Chem. Soc. 119, 11102-3
38. + channel J. Mol. Biol. 350, 857-65
39. a acidic residues J. Phys. Chem. B 116, 7145-53
40. Bayley, H., Cronin, B., Heron, A., Holden, M. A., Hwang, W. L., Syeda, R., Thompson, J., and Wallace, M. (2008) Droplet interface bilayers Mol. Biosyst. 4, 1191-208
41. Le Pioufle, B., Suzuki, H., Tabata, K. V., Noji, H., and Takeuchi, S. (2008) Lipid bilayer microarray for parallel recording of transmembrane ion currents Anal. Chem. 80, 328-32