Thermally responsive microcarriers with optimal poly(N-isopropylacrylamide) grafted density for facilitating cell adhesion/detachment in suspension culture

Acta Biomaterialia

Volumn 8, Issue 11, 2012, Pages 3904-3913

  Tamura, Atsushi ^a   Kobayashi, Jun ^a   Yamato, Masayuki ^a   Okano, Teruo ^a  

^a Tokyo Women s Medical University Waseda University Joint Institution for Advanced Biomedical Sciences   (Japan)

Author keywords

Atom transfer radical polymerization; Large scale culture; Microcarrier; Poly(N isopropylacrylamide); Polymer chain density

Indexed keywords

ACRYLIC MONOMERS; AMIDES; CELL ADHESION; CELL CULTURE; CELLS; CONTACT ANGLE; EFFICIENCY; GRAFTING (CHEMICAL); HYDROGELS;

ATOM-TRANSFER RADICAL POLYMERIZATION; CELLS ADHESION; CHAIN DENSITY; LARGE-SCALE CULTURES; MICROCARRIERS; POLY(N-ISOPROPYLACRYLAMIDE); POLYMER CHAIN DENSITY; POLYMER CHAINS; THERMALLY INDUCED;

ATOM TRANSFER RADICAL POLYMERIZATION;

FIBRONECTIN; POLY(N ISOPROPYLACRYLAMIDE); PROTEINASE; ACRYLAMIDE DERIVATIVE; CHLORINE; MICROSPHERE; POLY(N-ISOPROPYLACRYLAMIDE); POLYMER;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL DETACHMENT; CONTACT ANGLE; DENSITY; FEMALE; MICROCARRIER CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SURFACE PROPERTY; SUSPENSION CELL CULTURE; TEMPERATURE; WETTABILITY; ADSORPTION; ANIMAL; CHEMISTRY; CHO CELL; CULTURE TECHNIQUE; DRUG EFFECT; HAMSTER; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; PHASE CONTRAST MICROSCOPY; SUSPENSION; SYNTHESIS;

CRICETULUS GRISEUS;

ACRYLAMIDES; ADSORPTION; ANIMALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CHLORINE; CHO CELLS; CRICETINAE; FIBRONECTINS; MICROSCOPY, PHASE-CONTRAST; MICROSPHERES; MOLECULAR WEIGHT; POLYMERS; SURFACE PROPERTIES; SUSPENSIONS; TEMPERATURE;

EID: 84873969213     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2012.07.006     Document Type: Article

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