Temperature-responsive poly(N-isopropylacrylamide)-grafted microcarriers for large-scale non-invasive harvest of anchorage-dependent cells

Biomaterials

Volumn 33, Issue 15, 2012, Pages 3803-3812

  Tamura, Atsushi ^a   Kobayashi, Jun ^a   Yamato, Masayuki ^a   Okano, Teruo ^a  

^a Tokyo Women s Medical University Waseda University Joint Institution for Advanced Biomedical Sciences   (Japan)

Author keywords

Atom transfer radical polymerization; Large scale culture; Microcarrier; Poly(N isopropylacrylamide); Suspension culture; Temperature sensitivity

Indexed keywords

ADHERING CELLS; ANCHORAGE-DEPENDENT CELLS; BEAD SURFACE; CELL CULTIVATION; CELL DETACHMENT; CELL THERAPY; CHINESE HAMSTER OVARY; CULTURED CELL; GRAFTED CHAINS; INTACT CELLS; MICROCARRIER; MICROCARRIERS; N-ISOPROPYLACRYLAMIDES; PROTEOLYTIC ENZYME; SUSPENSION CULTURE; SUSPENSION CULTURES; TEMPERATURE DEPENDENT; TEMPERATURE-RESPONSIVE; TEMPERATURE-SENSITIVITY;

ACRYLIC MONOMERS; ATOM TRANSFER RADICAL POLYMERIZATION; CELL ADHESION; CELL PROLIFERATION; CELLS; GRAFTING (CHEMICAL);

CELL CULTURE;

CHLOROMETHYLATED POLY(STYRENE) BEAD; POLY(N ISOPROPYLACRYLAMIDE); POLYMER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ATOM TRANSFER RADICAL POLYMERIZATION; CELL ADHESION; CELL CULTURE; CELL PROLIFERATION; CHO CELL; FEMALE; NONHUMAN; PRIORITY JOURNAL; SUSPENSION CELL CULTURE; SYNTHESIS; TEMPERATURE;

ACRYLAMIDES; ANIMALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; MICROSCOPY, ELECTRON, SCANNING; MICROSPHERES; PHOTOELECTRON SPECTROSCOPY; POLYMERIZATION; POLYMERS; POLYSTYRENES; SURFACE PROPERTIES; TEMPERATURE; TIME FACTORS; WATER;

CRICETULUS GRISEUS;

EID: 84858288134     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2012.01.060     Document Type: Article

References (38)

1. Lutolf M.P., Hubbell J.A. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 2005, 23(1):47-55.
2. Körbling M., Estrov Z. Adult stem cells for tissue repair - A new therapeutic concept?. New Engl J Med 2003, 349(6):570-582.
3. Yang J., Yamato M., Kohno C., Nishimoto A., Sekine H., Fukai F., et al. Cell sheet engineering: Recreating tissues without biodegradable scaffolds. Biomaterials 2005, 26(33):6415-6422.
4. Malda J., Frondoza C.G. Microcarriers in the engineering of cartilage and bone. Trends Biotechnol 2006, 24(7):299-304.
5. Kehoe D.E., Jing D., Lock L.T., Tzanakakis E.S. Scalable stirred-suspension bioreactor culture of human pluripotent stem cells. Tissue Eng Part A 2010, 16(2):405-421.
6. Dang S.M., Gerecht-Nir S., Chen J., Itskovitz-Eldor J., Zandstra P.W. Controlled, scalable embryonic stem cell differentiation culture. Stem Cells 2004, 22(3):275-282.
7. Steiner D., Khaner H., Cohen M., Even-Ram S., Gil Y., Itsykson P., et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol 2010, 28(4):361-364.
8. Amit M., Laevsky I., Miropolsky Y., Shariki K., Peri M., Itskovitz-Eldor J., et al. Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells. Nat Protoc 2011, 6(5):572-579.
9. Zweigerdt R., Olmer R., Singh H., Haverich A., Martin U. Scalable expansion of human pluripotent stem cells in suspension culture. Nat Protoc 2011, 6(5):689-700.
10. van Wezel A.L. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature 1967, 216(5110):64-65.
11. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 2004, 22(11):1393-1398.
12. Glacken W.M., Fleischaker R.J., Sinskey A.J. Large-scale production of mammalian cells and their products: engineering principles and barriers to scale-up. Ann NY Acad Sci 1983, 413:355-372.
13. Martin Y., Eldardiri M., Lawrence-Watt D.J., Sharpe J.R. Microcarriers and their potential in tissue regeneration. Tissue Eng Part B 2011, 17(1):71-80.
14. Frondoza C., Sohrabi A., Hungerford D. Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture. Biomaterials 1996, 17(9):879-888.
15. Overstreet M., Sohrabi A., Polotsky A., Hungerford D.S., Frondoza C.G. Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins. In Vitro Cell Dev Biol Anim 2003, 39(5-6):228-234.
16. Yang Y., Rossi F.M.V., Putnins E.E. Ex vivo expansion of rat bone marrow mesenchymal stromal cells on microcarrier beads in spin culture. Biomaterials 2007, 28(20):3110-3120.
17. Tseng P.C., Young T.H., Wang T.M., Peng H.E., Hou S.M., Yen M.L. Spontaneous osteogenesis of MSCs cultured on 3D microcarriers through alteration of cytoskeletal tension. Biomaterials 2012, 33(2):556-564.
18. Alfred R., Taiani J.T., Krawetz R.J., Yamashita A., Rancourt D.E., Kallos M.S. Large-scale production of murine embryonic stem cell-derived osteoblasts and chondrocytes on microcarriers in serum-free media. Biomaterials 2011, 32(26):6006-6016.
19. Kushida A., Yamato M., Konno C., Kikuchi A., Sakurai Y., Okano T. Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces. J Biomed Mater Res 1999, 45(4):355-362.
20. Yamato M., Utsumi M., Kushida A., Konno C., Kikuchi A., Okano T. Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue Eng 2001, 7(4):473-480.
21. Okano T., Yamada N., Sakai H., Sakurai Y. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide). J Biomed Mater Res 1993, 27(10):1243-1251.
22. Yamada N., Okano T., Sakai H., Karikusa F., Sawasaki Y., Sakurai Y. Thermo-responsive polymeric surfaces; control of attachment and detachment of cultured cells. Makromol Chem Rapid Commun 1990, 11(11):571-576.
23. Yamato M., Akiyama Y., Kobayashi J., Yang J., Kikuchi A., Okano T. Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering. Prog Polym Sci 2007, 32(8-9):1123-1133.
24. Queffelec J., Gaynor S.G., Matyjaszewski K. Optimization of atom transfer radical polymerization using Cu(I)/tris(2-(dimethylamino)ethyl)amine as a catalyst. Macromolecules 2000, 33(23):8629-8639.
25. Nagase K., Kobayashi J., Kikuchi A., Akiyama Y., Kanazawa H., Okano T. Effects of graft densities and chain lengths on separation of bioactive compounds by nanolayered thermoresponsive polymer brush surfaces. Langmuir 2008, 24(2):511-517.
26. Mizutani A., Nagase K., Kikuchi A., Kanazawa H., Akiyama Y., Kobayashi J., et al. Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography. J Chromatogr A 2010, 1217(4):522-529.
27. Hórvölgyi Z., Németh S., Fendler J.H. Monoparticulate layers of silanized glass spheres at the water-air interface: Particle-particle and particle-subphase interactions. Langmuir 1996, 12(4):997-1004.
28. Bordács S., Agod A., Hórvölgyi Z. Compression of Langmuir films composed of fine particles: collapse mechanism and wettability. Langmuir 2006, 22(16):6944-6950.
29. Akiyama Y., Kikuchi A., Yamato M., Okano T. Ultrathin poly(N-isopropylacrylamide) grafted layer on polystyrene surfaces for cell adhesion/detachment control. Langmuir 2004, 20(13):5506-5511.
30. Takahashi H., Nakayama M., Yamato M., Okano T. Controlled chain length and graft density of thermoresponsive polymer brushes for optimizing cell sheet harvest. Biomacromolecules 2010, 11(8):1991-1999.
31. Mizutani A., Kikuchi A., Yamato M., Kanazawa H., Okano T. Preparation of thermoresponsive polymer brush surfaces and their interaction with cells. Biomaterials 2008, 29(13):2073-2081.
32. Matyjaszewski K., Xia J. Atom transfer radical polymerization. Chem Rev 2001, 101(9):2921-2990.
33. Tsujii Y., Ohno K., Yamamoto S., Goto A., Fukuda T. Structure and properties of high-density polymer brushes prepared by surface-initiated living radical polymerization. Adv Polym Sci 2006, 197:1-45.
34. Xia Y., Yin X., Burke N.A.D., Stöver H.D.H. Thermal response of narrow-disperse poly(N-isopropylacrylamide) prepared by atom transfer radical polymerization. Macromolecules 2005, 38(14):5937-5943.
35. Pan Y.V., Wesley R.A., Luginbuhl R., Denton D.D., Ratner B.D. Plasma polymerized N-isopropylacrylamide: synthesis and characterization of a smart thermally responsive coating. Biomacromolecules 2001, 2(1):32-36.
36. Maeda Y., Higuchi T., Ikeda I. Change in hydration state during the coil-globule transition of aqueous solutions of poly(N-isopropylacrylamide) as evidenced by FTIR spectroscopy. Langmuir 2000, 16(19):7503-7509.
37. Chu L., Robinson D.K. Industrial choices for protein production by large-scale cell culture. Curr Opin Biotech 2001, 12(2):180-187.
38. Plunkett K.N., Zhu X., Moore J.S., Leckband D.E. PNIPAM chain collapse depends on the molecular weight and grafting density. Langmuir 2006, 22(9):4259-4266.