Investigation of the antimicrobial effect of neosartorya fischeri antifungal protein (NFAP) after heterologous expression in aspergillus nidulans

Microbiology (United Kingdom)

Volumn 159, Issue 2, 2013, Pages 411-419

  Galgóczy, László ^a   Kovács, Laura ^a   Karácsony, Zoltán ^a   Virágh, Máté ^a   Hamari, Zsuzsanna ^a   Vágvölgyi, Csaba ^a  

^a UNIVERSITY OF SZEGED   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; CATION; CHITIN; DICHLORODIHYDROFLUORESCEIN DIACETATE; FLUORESCEIN ISOTHIOCYANATE; FUNGAL PROTEIN; LIPOCORTIN 5; NEOSARTORYA FISCHERI ANTIFUNGAL PROTEIN; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; APOPTOSIS; ARTICLE; ASPERGILLUS NIDULANS; CELL DEATH; CELL DESTRUCTION; CELL NUCLEUS; CELL WALL; CONIDIUM; CONTROLLED STUDY; FUNGAL GENE; FUNGAL STRAIN; FUNGUS HYPHAE; GENE EXPRESSION; GERMINATION; HETEROLOGOUS EXPRESSION; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; STAINING; SURVIVAL RATE;

ANTIFUNGAL AGENTS; APOPTOSIS; ASPERGILLUS NIDULANS; FUNGAL PROTEINS; HYPHAE; MICROSCOPY, ELECTRON, SCANNING; NEOSARTORYA; REACTIVE OXYGEN SPECIES; RECOMBINANT PROTEINS;

EID: 84873671620     PISSN: 13500872     EISSN: 14652080     Source Type: Journal    
DOI: 10.1099/mic.0.061119-0     Document Type: Article

References (19)

1. Brown, K. L. & Hancock, R. E. W. (2006). Cationic host defense (antimicrobial) peptides. Curr Opin Immunol 18, 24-30.
2. Hagen, S, Marx, F, Ram, A. F. & Meyer, V. (2007). The antifungal protein AFP from Aspergillus giganteus inhibits chitin synthesis in sensitive fungi. Appl Environ Microbiol 73, 2128-2134.
3. Hajji, M., Jellouli, K., Hmidet, N., Balti, R., Sellami-Kamoun, A. & Nasri, M. (2010). A highly thermostable antimicrobial peptide from Aspergillus clavatus ES1: biochemical and molecular characterization. J Ind Microbiol Biotechnol 37, 805-813.
4. Kaiserer, L, Oberparleiter, C, Weiler-Gorz, R., Burgstaller, W., Leiter, E. & Marx, F. (2003). Characterization of the Penicillium chrysogenum antifungal protein PAF. Arch Microbiol 180, 204-210.
5. Kaminskyj, S. G. W. (2001). Fundamentals of growth, storage, genetics and microscopy of Aspergillus nidulans. Fungal Genet Newsl 48, 25-31.
6. Kovacs, L, Viragh, M., Tako, M., Papp, T., Vagvolgyi, C. & Galgoczy, L. (2011). Isolation and characterization of Neosartorya fischeri antifungal protein (NFAP). Peptides 32, 1724-1731.
7. Kovacs, L, Viragh, M., Tako, M., Papp, T., Vagvolgyi, C. & Galgoczy, L. (2011). Antifungal activity of the Neosartorya fischeri antifungal protein (NFAP) against filamentous fungal isolates from clinical sources. Clin Microbiol Infect 17 (S4), 327.
8. Krizsan, K., Bencsik, O., Nyilasi, I., Galgoczy, L, Vagvolgyi, C. & Papp, T. (2010). Effect of the sesterterpene-type metabolites, ophiobolins A and B, on zygomycetes fungi. FEMS Microbiol Lett 313, 135-140.
9. Leiter, E., Szappanos, H., Oberparleiter, C, Kaiserer, L, Csernoch, L., Pusztahelyi, T., Emri, T, Pocsi, I., Salvenmoser, W. & Marx, F. (2005). Antifungal protein PAF severely affects the integrity of the plasma membrane of Aspergillus nidulans and induces an apoptosislike phenotype. Antimicrob Agents Chemother 49, 2445-2453.
10. Marx, F. (2004). Small, basic antifungal proteins secreted from filamentous ascomycetes: a comparative study regarding expression, structure, function and potential application. Appl Microbiol Biotechnol 65, 133-142.
11. Marx, F., Binder, U., Leiter, E. & Pocsi, I. (2008). The Penicillium chrysogenum antifungal protein PAF, a promising tool for the development of new antifungal therapies and fungal cell biology studies. Cell Mol Life Sci 65, 445-454.
12. Meyer, V. (2008). A small protein that fights fungi: AFP as a new promising antifungal agent of biotechnological value. Appl Microbiol Biotechnol 78, 17-28.
13. Osherov, N. & May, G. (2000). Conidial germination in Aspergillus nidulans requires RAS signaling and protein synthesis. Genetics 155, 647-656.
14. Pokorska, A., Drevet, C. & Scazzocchio, C. (2000). The analysis of the transcriptional activator PrnA reveals a tripartite nuclear localisation sequence. J Mol Biol 298, 585-596.
15. Theis, T. & Stahl, U. (2004). Antifungal proteins: targets, mechanisms and prospective applications. Cell Mol Life Sci 61, 437-455.
16. Theis, T., Wedde, M., Meyer, V. & Stahl, U. (2003). The antifungal protein from Aspergillus giganteus causes membrane permeabilization. Antimicrob Agents Chemother 47, 588-593.
17. Theis, T., Marx, F., Salvenmoser, W., Stahl, U. & Meyer, V. (2005). New insights into the target site and mode of action of the antifungal protein of Aspergillus giganteus. Res Microbiol 156, 47-56.
18. Tilburn, J., Scazzocchio, C, Taylor, G. G., Zabicky-Zissman, J. H., Lockington, R. A. & Davies, R. W. (1983). Transformation by integration in Aspergillus nidulans. Gene 26, 205-221.
19. Yu, J. H., Hamari, Z., Han, K. H., Seo, J. A., Reyes-Domiínguez, Y. & Scazzocchio, C. (2004). Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet Biol 41, 973-981.