Utilization of fast qPCR techniques to detect the amphibian chytrid fungus: A cheaper and more efficient alternative method

Methods in Ecology and Evolution

Volumn 4, Issue 2, 2013, Pages 162-166

  Kerby, Jacob L ^a   Schieffer, Alicia ^a   Brown, Jennifer R ^a   Whitfield, Steven ^a  

^a University of South Dakota   (United States)

Author keywords

Amphibian conservation; Chytridiomycosis; Diagnosis; Quantitative PCR

Indexed keywords

AMPHIBIA; ANIMALIA; BATRACHOCHYTRIUM DENDROBATIDIS; FUNGI;

EID: 84873548139     PISSN: None     EISSN: 2041210X     Source Type: Journal    
DOI: 10.1111/j.2041-210x.2012.00263.x     Document Type: Article

References (21)

1. Annis, S.L., Dastoor, F.P., Ziel, H., Daszak, P. & Longcore, J.E. (2004) A DNA-based assay identifies Batrachochytrium dendrobatidis in amphibians. Journal of Wildlife Diseases, 40, 420-428.
2. Berger, L., Speare, R., Daszak, P., Green, D., Cunningham, A., Goggin, C., Slocombe, R., Ragan, M., Hyatt, A. & McDonald, K. (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proceedings of the National Academy of Sciences of the United States of America, 95, 9031.
3. Boyle, D., Boyle, D., Olsen, V., Morgan, J. & Hyatt, A. (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Diseases of Aquatic Organisms, 60, 141-148.
4. Cheng, T.L., Rovito, S.M., Wake, D.B. & Vredenburg, V.T. (2011) Coincident mass extirpation of neotropical amphibians with the emergence of the infectious fungal pathogen Batrachochytrium dendrobatidis. Proceedings of the National Academy of Sciences, 108, 9502.
5. Hilscher, C., Vahrson, W. & Dittmer, D.P. (2005) Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability. Nucleic Acids Research, 33, e182.
6. Hyatt, A., Boyle, D., Olsen, V., Boyle, D., Berger, L., Obendorf, D., Dalton, A., Kriger, K., Hero, M. & Hines, H. (2007) Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms, 73, 175-192.
7. Kilpatrick, A.M., Briggs, C.J. & Daszak, P. (2010) The ecology and impact of chytridiomycosis: an emerging disease of amphibians. Trends in Ecology & Evolution, 25, 109-118.
8. Kriger, K., Hines, H., Hyatt, A., Boyle, D. & Hero, J. (2006) Techniques for detecting chytridiomycosis in wild frogs: comparing histology with real-time Taqman PCR. Diseases of Aquatic Organisms, 71, 141.
9. Lips, K.R., Brem, F., Brenes, R., Reeve, J.D., Alford, R.A., Voyles, J., Carey, C., Livo, L., Pessier, A.P. & Collins, J.P. (2006) Emerging infectious disease and the loss of biodiversity in a Neotropical amphibian community. Proceedings of the National Academy of Sciences of the United States of America, 103, 3165-3170.
10. Prunier, J., Kaufmann, B., Grolet, O., Picard, D., Pompanon, F. & Joly, P. (2012) Skin swabbing as a new efficient DNA sampling technique in amphibians, and 14 new microsatellite markers in the alpine newt (Ichthyosaura alpestris). Molecular Ecology Resources, 12, 524-531.
11. Puschendorf, R., Bolaños, F. & Chaves, G. (2006) The amphibian chytrid fungus along an altitudinal transect before the first reported declines in Costa Rica. Biological Conservation, 132, 136-142.
12. Retallick, R.W.R., Miera, V., Richards, K.L., Field, K.J. & Collins, J.P. (2006) A non-lethal technique for detecting the chytrid fungus Batrachochytrium dendrobatidis on tadpoles. Diseases of Aquatic Organisms, 72, 77.
13. Richards-Hrdlicka, K.L. (2012) Extracting the amphibian chytrid fungus from formalin-fixed specimens. Methods in Ecology and Evolution, 3, 842-849.
14. Ruthig, G.R. & DeRidder, B.P. (2012) Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA. Diseases of Aquatic Organisms, 97, 249-253.
15. Skerratt, L.F., Mendez, D., McDonald, K.R., Garland, S., Livingstone, J., Berger, L. & Speare, R. (2011) Validation of diagnostic tests in wildlife: the case of chytridiomycosis in wild amphibians. Journal of Herpetology, 45, 444-450.
16. Smith, K.G., Lips, K.R. & Chase, J.M. (2009) Selecting for extinction: nonrandom disease-associated extinction homogenizes amphibian biotas. Ecology Letters, 12, 1069-1078.
17. Stuart, S., Chanson, J., Cox, N., Young, B., Rodrigues, A., Fischman, D. & Waller, R. (2004) Status and trends of amphibian declines and extinctions worldwide. Science, 306, 1783-1786.
18. Voyles, J., Berger, L., Young, S., Speare, R., Webb, R., Warner, J., Rudd, D., Campbell, R. & Skerratt, L.F. (2007) Electrolyte depletion and osmotic imbalance in amphibians with chytridiomycosis. Diseases of Aquatic Organisms, 77, 113.
19. Whitfield, S.M., Bell, K.E., Philippi, T., Sasa, M., Bolaños, F., Chaves, G., Savage, J.M. & Donnelly, M.A. (2007) Amphibian and reptile declines over 35 years at La Selva, Costa Rica. Proceedings of the National Academy of Sciences of the United States of America, 104, 8352.
20. Whitfield, S.M., Kerby, J.L., Gentry, L.R. & Donnelly, M.A. (2012) Temporal variation in the prevalence of chytridiomycosis in three species of frogs at La Selva, Costa Rica. Biotropica, DOI: 10.1111/j.1744-7429.2012.00872.x.
21. Yoder, K.E. & Fishel, R. (2008) Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles. Journal of Virological Methods, 153, 253-256.