Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles

Journal of Virological Methods

Volumn 153, Issue 2, 2008, Pages 253-256

  Yoder, Kristine E ^a   Fishel, Richard ^a  

^a Immunology   (United States)

Author keywords

Fast PCR; HIV; Quantitative PCR

Indexed keywords

ACCURACY; ARTICLE; HUMAN IMMUNODEFICIENCY VIRUS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; VIRUS DETECTION;

DNA, COMPLEMENTARY; DNA, VIRAL; HIV INFECTIONS; HIV LONG TERMINAL REPEAT; HIV-1; HUMANS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 53049095785     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.07.032     Document Type: Article

References (7)

1. Bushman F. Measuring covert HIV replication during HAART: the abundance of 2-LTR circles is not a reliable marker. Aids 17 (2003) 749-750
2. Butler S.L., Hansen M.S., and Bushman F.D. A quantitative assay for HIV DNA integration in vivo. Nat. Med. 7 (2001) 631-634
3. Coffin J.M., Hughes S.H., and Varmus H.E. Retroviruses (1997), Cold Spring Harbor Laboratory Press, Cold Spring Harbor
4. Desire N., Dehee A., Schneider V., Jacomet C., Goujon C., Girard P.M., Rozenbaum W., and Nicolas J.C. Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay. J. Clin. Microbiol. 39 (2001) 1303-1310
5. Hilscher C., Vahrson W., and Dittmer D.P. Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability. Nucleic Acids Res. 33 (2005) e182
6. Sharkey M.E., Teo I., Greenough T., Sharova N., Luzuriaga K., Sullivan J.L., Bucy R.P., Kostrikis L.G., Haase A., Veryard C., Davaro R.E., Cheeseman S.H., Daly J.S., Bova C., Ellison III R.T., Mady B., Lai K.K., Moyle G., Nelson M., Gazzard B., Shaunak S., and Stevenson M. Persistence of episomal HIV-1 infection intermediates in patients on highly active anti-retroviral therapy. Nat. Med. 6 (2000) 76-81
7. Shaunak S., Teo I., Choi J.W., and Gazzard B. HIV DNA two long terminal repeat circles: observations and interpretations. Aids 17 (2003) 2273-2274