One-step immobilization and purification of his-tagged enzyme using poly(2-acetamidoacrylic acid) hydrogel

Macromolecular Research

Volumn 21, Issue 1, 2013, Pages 5-9

  Ha, Eun Ju ^a   Kim, Kyeong Kyu ^b   Park, Hyung Soon ^c   Lee, Sun Gu ^a   Lee, Jang Oo ^a   Seong Soo, A A ^d   Paik, Hyun Jong ^a  

^a Pusan National University   (South Korea)

^b Samsung Biomedical Research Institute   (South Korea)

^c Probiond Co Ltd   (South Korea)

^d Gachon University College of Medicine   (South Korea)

Author keywords

His tagged enzyme.; Immobilization; Poly(2 acetamidoacrylic acid) hydrogel; Purification

Indexed keywords

AMINO PEPTIDASE; CELL LYSATES; FOUR CYCLES; HIS-TAG; IMMOBILIZED ENZYME; INITIAL ACTIVITY; POLY(2-ACETAMIDOACRYLIC ACID) HYDROGEL; POTASSIUM PERSULFATE;

AMINO ACIDS; CATALYST ACTIVITY; DIMETHYL SULFOXIDE; HYDROGELS; ORGANIC SOLVENTS; POTASSIUM; PURIFICATION; RADIOACTIVE WASTE VITRIFICATION;

ENZYME IMMOBILIZATION;

EID: 84873413560     PISSN: 15985032     EISSN: 20927673     Source Type: Journal    
DOI: 10.1007/s13233-013-1007-8     Document Type: Article

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