Characterization of dominant-negative and temperature-sensitive mutants of tombusvirus replication proteins affecting replicase assembly

Virology

Volumn 437, Issue 1, 2013, Pages 48-61

  Pathak, Kunj B ^a   Jiang, Zuodong ^a   Ochanine, Verena ^a   Sharma, Monika ^a   Pogany, Judit ^a   Nagy, Peter D ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Cell free; Cucumber necrosis virus; Dominant negative mutant; In vitro; Recombination; Replicase assembly; Replication; Temperature sensitive; Tombusvirus; Yeast

Indexed keywords

MUTANT PROTEIN; P33 REPLICATION PROTEIN; P92 RDRP PROTEIN; P92 REPLICATION PROTEIN; RNA DIRECTED RNA POLYMERASE; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CONTROLLED STUDY; CUCUMBER NECROSIS VIRUS; IN VITRO STUDY; LOSS OF FUNCTION MUTATION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; RNA RECOMBINATION; RNA REPLICATION; RNA SYNTHESIS; SACCHAROMYCES CEREVISIAE; TOMBUSVIRUS; VIRUS RECOMBINATION;

POINT MUTATION; PROTEIN INTERACTION DOMAINS AND MOTIFS; RECOMBINATION, GENETIC; RNA REPLICASE; RNA, VIRAL; SACCHAROMYCES CEREVISIAE; TEMPERATURE; TOMBUSVIRUS; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

CUCUMBER NECROSIS VIRUS; RNA VIRUSES; TOMBUSVIRUS;

EID: 84873089242     PISSN: 00426822     EISSN: 10960341     Source Type: Journal    
DOI: 10.1016/j.virol.2012.12.009     Document Type: Article

References (54)

1. Barajas D., Jiang Y., Nagy P.D. A unique role for the host ESCRT proteins in replication of tomato bushy stunt virus. PLoS Pathog. 2009, 5(12):e1000705.
2. Barajas D., Nagy P.D. Ubiquitination of tombusvirus p33 replication protein plays a role in virus replication and binding to the host Vps23p ESCRT protein. Virology 2010, 397(2):358-368.
3. Bartenschlager R., Cosset F.L., Lohmann V. Hepatitis C virus replication cycle. J. Hepatol. 2010, 53(3):583-585.
4. Cheng C.P., Nagy P.D. Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro. J. Virol. 2003, 77(22):12033-12047.
5. den Boon J.A., Ahlquist P. Organelle-like membrane compartmentalization of positive-strand RNA virus replication factories. Annu. Rev. Microbiol. 2010, 64:241-256.
6. den Boon J.A., Diaz A., Ahlquist P. Cytoplasmic viral replication complexes. Cell Host Microbe 2010, 8(1):77-85.
7. Huang T.S., Nagy P.D. Direct inhibition of tombusvirus plus-strand RNA synthesis by a dominant negative mutant of a host metabolic enzyme, glyceraldehyde-3-phosphate dehydrogenase, in yeast and plants. J. Virol. 2011, 85(17):9090-9102.
8. 2+ ion pump is a factor in the emergence of viral RNA recombinants. Cell Host Microbe 2010, 7(1):74-81.
9. Kovalev N., Pogany J., Nagy P.D. A co-opted DEAD-box RNA helicase enhances tombusvirus plus-strand synthesis. PLoS Pathog. 2012, 8(2):e1002537.
10. Li Z., Barajas D., Panavas T., Herbst D.A., Nagy P.D. Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex and ubiquitinates p33 replication protein. J. Virol. 2008, 82(14):6911-6926.
11. Li Z., Nagy P.D. Diverse roles of host RNA binding proteins in RNA virus replication. RNA Biol. 2011, 8(2):305-315.
12. Li Z., Pogany J., Panavas T., Xu K., Esposito A.M., Kinzy T.G., Nagy P.D. Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor. Virology 2009, 385(1):245-260.
13. Li Z., Pogany J., Tupman S., Esposito A.M., Kinzy T.G., Nagy P.D. Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis. PLoS Pathog. 2010, 6(11):e1001175.
14. McCartney A.W., Greenwood J.S., Fabian M.R., White K.A., Mullen R.T. Localization of the tomato bushy stunt virus replication protein p33 reveals a peroxisome-to-endoplasmic reticulum sorting pathway. Plant Cell 2005, 17(12):3513-3531.
15. Mendu V., Chiu M., Barajas D., Li Z., Nagy P.D. Cpr1 cyclophilin and Ess1 parvulin prolyl isomerases interact with the tombusvirus replication protein and inhibit viral replication in yeast model host. Virology 2010, 406(2):342-351.
16. Miller S., Krijnse-Locker J. Modification of intracellular membrane structures for virus replication. Nat. Rev. Microbiol. 2008, 6(5):363-374.
17. Monkewich S., Lin H.X., Fabian M.R., Xu W., Na H., Ray D., Chernysheva O.A., Nagy P.D., White K.A. The p92 polymerase coding region contains an internal RNA element required at an early step in Tombusvirus genome replication. J. Virol. 2005, 79(8):4848-4858.
18. Nagy P.D. Yeast as a model host to explore plant virus-host interactions. Annu. Rev. Phytopathol. 2008, 46:217-242.
19. Nagy P.D. The roles of host factors in tombusvirus RNA recombination. Adv. Virus Res. 2011, 81:63-84.
20. Nagy P.D., Barajas D., Pogany J. Host factors with regulatory roles in tombusvirus replication. Curr. Opin. Virol. 2012, 2(6):685-692.
21. Nagy P.D., Pogany J. Multiple roles of viral replication proteins in plant RNA virus replication. Methods Mol. Biol. 2008, 451:55-68.
22. Nagy P.D., Pogany J. Global genomics and proteomics approaches to identify host factors as targets to induce resistance against tomato bushy stunt virus. Adv. Virus Res. 2010, 76:123-177.
23. Nagy P.D., Pogany J. The dependence of viral RNA replication on co-opted host factors. Nat. Rev. Microbiol. 2011.
24. Nagy P.D., Pogany J. The dependence of viral RNA replication on co-opted host factors. Nat. Rev. Microbiol. 2012, 10(2):137-149.
25. Nagy P.D., Wang R.Y., Pogany J., Hafren A., Makinen K. Emerging picture of host chaperone and cyclophilin roles in RNA virus replication. Virology 2011, 411(2):374-382.
26. Novoa R.R., Calderita G., Arranz R., Fontana J., Granzow H., Risco C. Virus factories: associations of cell organelles for viral replication and morphogenesis. Biol. Cell 2005, 97(2):147-172.
27. Panavas T., Hawkins C.M., Panaviene Z., Nagy P.D. The role of the p33:p33/p92 interaction domain in RNA replication and intracellular localization of p33 and p92 proteins of Cucumber necrosis tombusvirus. Virology 2005, 338(1):81-95.
28. Panavas T., Nagy P.D. Yeast as a model host to study replication and recombination of defective interfering RNA of Tomato bushy stunt virus. Virology 2003, 314(1):315-325.
29. Panavas T., Pogany J., Nagy P.D. Internal initiation by the cucumber necrosis virus RNA-dependent RNA polymerase is facilitated by promoter-like sequences. Virology 2002, 296(2):275-287.
30. Panaviene Z., Panavas T., Nagy P.D. Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase. J. Virol. 2005, 79(16):10608-10618.
31. Panaviene Z., Panavas T., Serva S., Nagy P.D. Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity. J. Virol. 2004, 78(15):8254-8263.
32. Pathak K.B., Pogany J., Xu K., White K.A., Nagy P.D. Defining the roles of cis-acting RNA Elements in Tombusvirus replicase assembly in vitro. J. Virol. 2012, 86(1):156-171.
33. Pathak K.B., Sasvari Z., Nagy P.D. The host Pex19p plays a role in peroxisomal localization of tombusvirus replication proteins. Virology 2008, 379(2):294-305.
34. Pogany J., Nagy P.D. Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast. J. Virol. 2008, 82(12):5967-5980.
35. Pogany J., Nagy P.D. p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of tomato bushy stunt virus in yeast cell-free extract. J. Virol. 2012, 86(22):12025-12038.
36. Pogany J., Stork J., Li Z., Nagy P.D. In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70. Proc. Natl. Acad. Sci. USA 2008, 105(50):19956-19961.
37. Pogany J., White K.A., Nagy P.D. Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication. J. Virol. 2005, 79(8):4859-4869.
38. Rajendran K.S., Nagy P.D. Characterization of the RNA-binding domains in the replicase proteins of tomato bushy stunt virus. J. Virol. 2003, 77(17):9244-9258.
39. Rajendran K.S., Nagy P.D. Interaction between the replicase proteins of Tomato bushy stunt virus in vitro and in vivo. Virology 2004, 326(2):250-261.
40. Rajendran K.S., Nagy P.D. Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: requirement of p33:p92 interaction for replicase assembly. Virology 2006, 345(1):270-279.
41. Rajendran K.S., Pogany J., Nagy P.D. Comparison of turnip crinkle virus RNA-dependent RNA polymerase preparations expressed in Escherichia coli or derived from infected plants. J. Virol. 2002, 76(4):1707-1717.
42. Salonen A., Ahola T., Kaariainen L. Viral RNA replication in association with cellular membranes. Curr. Top Microbiol. Immunol. 2005, 285:139-173.
43. Sasvari Z., Izotova L., Kinzy T.G., Nagy P.D. Synergistic roles of eukaryotic translation elongation factors 1Bgamma and 1A in stimulation of tombusvirus minus-strand synthesis. PLoS Pathog. 2011, 7(12):e1002438.
44. Serva S., Nagy P.D. Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication. J. Virol. 2006, 80(5):2162-2169.
45. Serviene E., Jiang Y., Cheng C.P., Baker J., Nagy P.D. Screening of the yeast yTHC collection identifies essential host factors affecting tombusvirus RNA recombination. J. Virol. 2006, 80(3):1231-1241.
46. Serviene E., Shapka N., Cheng C.P., Panavas T., Phuangrat B., Baker J., Nagy P.D. Genome-wide screen identifies host genes affecting viral RNA recombination. Proc. Natl. Acad. Sci. U S A 2005, 102(30):10545-10550.
47. Shapka N., Nagy P.D. The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: implications for the mechanism of RNA recombination. J. Virol. 2004, 78(5):2288-2300.
48. Shapka N., Stork J., Nagy P.D. Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication. Virology 2005, 343(1):65-78.
49. Shi S.T., Lai M.M. Viral and cellular proteins involved in coronavirus replication. Curr. Top Microbiol. Immunol. 2005, 287:95-131.
50. Stork J., Kovalev N., Sasvari Z., Nagy P.D. RNA chaperone activity of the tombusviral p33 replication protein facilitates initiation of RNA synthesis by the viral RdRp in vitro. Virology 2011, 409(2):338-347.
51. Wang R.Y., Nagy P.D. Tomato bushy stunt virus co-opts the RNA-binding function of a host metabolic enzyme for viral genomic RNA synthesis. Cell Host Microbe 2008, 3(3):178-187.
52. Wang R.Y., Stork J., Nagy P.D. A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteins to intracellular membranes. J. Virol. 2009, 83(7):3276-3287.
53. Wang R.Y., Stork J., Pogany J., Nagy P.D. A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication. Virology 2009, 394(1):28-38.
54. White K.A., Nagy P.D. Advances in the molecular biology of tombusviruses: gene expression, genome replication, and recombination. Prog. Nucleic Acid Res. Mol. Biol. 2004, 78:187-226.