Structure and protein adsorption mechanisms of clean and fouled tentacle-type anion exchangers used in a monoclonal antibody polishing step

Journal of Chromatography A

Volumn 1278, Issue , 2013, Pages 116-125

  Corbett, Rachel ^a   Carta, Giorgio ^a   Iskra, Timothy ^b   Gallo, Christopher ^b   Godavarti, Ranga ^b   Salm, Jeffrey R ^b  

^a University of Virginia   (United States)

^b PFIZER INC   (United States)

Author keywords

Anion exchange resins; Fouling; Mass transfer mechanisms; Monoclonal antibodies; Polishing

Indexed keywords

ANION EXCHANGE RESINS; ANION EXCHANGERS; BIMODAL DISTRIBUTION; BINDING CAPACITIES; BSA ADSORPTION; CONFOCAL LASER SCANNING MICROSCOPY; CORE STRUCTURE; EXTERIOR SURFACES; INTRAPARTICLE; INVERSE SIZE EXCLUSION CHROMATOGRAPHY; MASS TRANSFER MECHANISM; MICROGRANULAR STRUCTURE; MONOCLONAL ANTIBODIES (MAB); POLISHING STEPS; PROTEIN ADSORPTION; PROTEIN CONCENTRATIONS; PROTEIN TRANSPORT; PURIFICATION PROCESS; RESIN SAMPLES; SKIN LAYER; THYROGLOBULIN; TRANSMISSION ELECTRON MICROGRAPH;

ADSORPTION; AGGLOMERATION; CONFOCAL MICROSCOPY; GLYCOPROTEINS; ION EXCHANGE RESINS; MONOCLONAL ANTIBODIES; POLISHING; PORE SIZE; PROTEINS;

FOULING;

ANION EXCHANGE RESIN; BOVINE SERUM ALBUMIN; MONOCLONAL ANTIBODY; THYROGLOBULIN;

ADSORPTION KINETICS; ARTICLE; BINDING AFFINITY; BIOFOULING; CHEMICAL STRUCTURE; CONFOCAL LASER MICROSCOPY; DIFFUSION; GEL PERMEATION CHROMATOGRAPHY; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN TRANSPORT; TRANSMISSION ELECTRON MICROSCOPY;

ADSORPTION; ANION EXCHANGE RESINS; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; KINETICS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR STRUCTURE; PROTEINS;

EID: 84873088575     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2013.01.006     Document Type: Article

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