Detection of heterogeneity of borrelia burgdorferi in ixodes ticks by culture-dependent and culture-independent methods

Journal of Clinical Microbiology

Volumn 51, Issue 2, 2013, Pages 615-617

  Xu, Guang ^a   Wesker, Jaime ^a   White, Caitlin ^a   Campbell, Jason ^a   Reich, Nicholas G ^a   Rich, Stephen M ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GENE; BORRELIA BURGDORFERI; CONTROLLED STUDY; CULTURE TECHNIQUE; DIRECT POLYMERASE CHAIN REACTION; DNA EXTRACTION; GENETIC HETEROGENEITY; GENETIC VARIABILITY; GENOTYPE; IN VITRO CULTURE; INFECTION RATE; INTERMETHOD COMPARISON; IXODES; LYME DISEASE; NONHUMAN; OSPC GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BORRELIA BURGDORFERI; FEMALE; GENE FREQUENCY; GENETIC VARIATION; GENOTYPE; IXODES; MALE;

EID: 84873054887     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03009-12     Document Type: Article

References (12)

1. Wormser GP, Wang G. 2011. The role of culture and nucleic acid amplification in diagnosis of lyme borreliosis, p 159-184. In Sood SK (ed), Lyme borreliosis in Europe and North America: epidemiology and clinical practice. John Wiley & Sons, Inc, Hoboken, NJ.
2. Barbour AG. 1984. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med. 57:521-525.
3. Pollack RJ, Telford SR, III, Spielman A. 1993. Standardization of medium for culturing Lyme disease spirochetes. J. Clin. Microbiol. 31:1251- 1255.
4. Preac-Mursic V, Wilske B, Schierz G. 1986. European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility. Zentralbl Bakteriol. Mikrobiol. Hyg. A 263:112-118.
5. Walk ST, Xu G, Stull JW, Rich SM. 2009. Correlation between tick density and pathogen endemicity, New Hampshire. Emerg. Infect. Dis. 15:585-587.
6. Lin T, Oliver JH Jr, Gao L. 2002. Genetic diversity of the outer surface protein C gene of southern Borrelia isolates and its possible epidemiological, clinical, and pathogenetic implications. J. Clin. Microbiol. 40:2572- 2583.
7. Qiu WG, Dykhuizen DE, Acosta MS, Luft BJ. 2002. Geographic uniformity of the Lyme disease spirochete (Borrelia burgdorferi) and its shared history with tick vector (Ixodes scapularis) in the Northeastern United States. Genetics 160:833- 849.
8. Seinost G, Dykhuizen DE, Dattwyler RJ, Golde WT, Dunn JJ, Wang IN, Wormser GP, Schriefer ME, Luft BJ. 1999. Four clones of Borrelia burgdorferi sensu stricto cause invasive infection in humans. Infect. Immun. 67:3518 -3524.
9. Wang IN, Dykhuizen DE, Qiu W, Dunn JJ, Bosler EM, Luft BJ. 1999. Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto. Genetics 151:15-30.
10. Wormser GP, Brisson D, Liveris D, Hanincova K, Sandigursky S, Nowakowski J, Nadelman RB, Ludin S, Schwartz I. 2008. Borrelia burgdorferi genotype predicts the capacity for hematogenous dissemination during early Lyme disease. J. Infect. Dis. 198:1358 -1364.
11. Rijpkema SG, Molkenboer MJ, Schouls LM, Jongejan F, Schellekens JF. 1995. Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol. 33:3091-3095.
12. Efron B. 1981. Nonparametric estimates of standard error-the jackknife, the bootstrap and other methods. Biometrika 68:589 -599.