Comparison of commercial real-Time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different sample types

Journal of Clinical Microbiology

Volumn 51, Issue 2, 2013, Pages 547-556

  Gerber, Priscilla F ^a   O'Neill, Kevin ^a   Owolodun, Olajide ^a   Wang, Chong ^a,b   Harmon, Karen ^a   Zhang, Jianqiang ^a   Halbur, Patrick G ^a   Zhou, Lei ^c   Meng, Xiang Jin ^c   Opriessnig, Tanja ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b Department of Statistics Center for Statistics and Applications in Forensic Evidence Iowa State University 2438 Osborn Dr Ames   (United States)

^c VIRGINIA MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS ANTIBODY; VIRUS RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTERIVIRUS; ARTICLE; BLOOD SMEAR; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; IMMUNOASSAY; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SERUM; SPERM; STRAIN IDENTIFICATION; SWINE; TURNAROUND TIME; VIRUS IDENTIFICATION; VIRUS ISOLATION; VIRUS STRAIN;

ANIMALS; ANTIBODIES, VIRAL; BODY FLUIDS; ENZYME-LINKED IMMUNOSORBENT ASSAY; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SWINE; VIRAL ENVELOPE PROTEINS;

EID: 84873019859     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02685-12     Document Type: Article

References (27)

1. Corzo CA, Mondaca E, Wayne S, Torremorell M, Dee S, Davies P, Morrison RB. 2010. Control and elimination of porcine reproductive and respiratory syndrome virus. Virus Res. 154:185-192.
2. Shi M, Lam TT, Hon CC, Hui RK, Faaberg KS, Wennblom T, Murtaugh MP, Stadejek T, Leung FC. 2010. Molecular epidemiology of PRRSV: a phylogenic perspective. Virus Res. 154:7-17.
3. Harmon KM, Abate SA, Chriswell AJ, Strait WAE. 2012. Comparison of two commercial real-time reverse transcriptase polymerase chain reaction assays for porcine reproductive and respiratory syndrome virus. Swine Health Prod. 20:184 -188.
4. Toplak I, Rithtaric D, Hostnik J, Grom J, Stukelj M, Valencak Z. 2012. Identification of a genetically diverse sequence of porcine reproductive and respiratory syndrome virus in Slovenia and the impact on the sensitivity of four molecular tests. J. Virol. Methods 179:51-56.
5. Wernike K, Bonilauri P, Dauber M, Errington J, LeBlanc N, Revilla- Fernández S, Hjulsager C, Isaksson M, Stadejek T, Beer M, Hoffmann B. 2012. Porcine reproductive and respiratory syndrome virus. Interlaboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction detection method. J. Vet. Diagn. Invest. 24:855- 866.
6. Kittawornrat A, Prickett J, Chittick W, Wang C, Engle M, Johnson J, Patnayak D, Schwartz T, Whitney D, Olsen C, Schwartz K, Zimmerman J. 2010. Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: will oral fluid replace serum for PRRSV surveillance? Virus Res. 154:170 -176.
7. Reicks DL, Muñoz-Zanzi C, Rossow K. 2006. Sampling of adult boars during early infection with porcine reproductive and respiratory syndrome virus for testing by polymerase chain reaction using new blood collection technique (blood swab method). Swine Health Prod. 14:258- 264.
8. Christopher-Hennings J, Holler LD, Benfield DA, Nelson EA. 2001. Detection and duration of porcine reproductive and respiratory syndrome virus in semen, serum, peripheral blood mononuclear cells, and tissues from Yorkshire, Hampshire, and Landrace boars. J. Vet. Diagn. Invest. 13:133-142.
9. Rovira A, Clement T, Christopher-Hennings J, Thompson B, Engle M, Reicks D, Muñoz-Zanzi C. 2007. Evaluation of the sensitivity of reversetranscription polymerase chain reaction to detect porcine reproductive and respiratory syndrome virus on individual and pooled samples from boars. J. Vet. Diagn. Invest. 19:502-509.
10. Johnson W, Roof M, Vaughn E, Christopher-Hennings J, Johnson CR, Murtaugh MP. 2004. Pathogenic and humoral immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) are related to viral load in acute infection. Vet. Immunol. Immunopathol. 102:233-247.
11. Fereidouni SA, Harder TC, Gaidet N, Ziller M, Hoffmann B, Hammoumi S, Globig A, Starick E. 2012. Saving resources: avian influenza surveillance using pooled swab samples and reduced reaction volumes in real-time RT-PCR. J. Virol. Methods 186:119 -125.
12. Ladman BS, Spackman E, Gelb J Jr. 2012. Comparison of pooling 11 or 5 oropharyngeal swabbings for detecting avian influenza virus by realtime reverse transcription-PCR in broiler chickens. Avian Dis. 56:227- 229.
13. Halbur PG, Paul PS, Frey ML, Landgraf J, Eernisse K, Meng XJ, Lum MA, Andrews JJ, Rathje JA. 1995. Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet. Pathol. 32:648-660.
14. Mengeling WL, Lager KM, Vorwald AC. 1998. Clinical consequences of exposing pregnant gilts to strains of porcine reproductive and respiratory syndrome (PRRS) virus isolated from field cases of "atypical" PRRS. Am. J. Vet. Res. 59:1540 -1544.
15. Gauger P, Faaberg KS, Guo B, Kappes MA, Opriessnig T. 2012. Genetic and phenotypic characterization of a 2006 United States porcine reproductive and respiratory virus isolate associated with high morbidity and mortality in the field. Virus Res. 163:98 -107.
16. Prickett J, Simer R, Christopher-Hennings J, Yoon KJ, Evans RB, Zimmerman JJ. 2008. Detection of Porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: a longitudinal study under experimental conditions. J. Vet. Diagn. Invest. 20: 156 -163.
17. Truong HM, Lu Z, Kutish GF, Galeota J, Osorio FA, Pattnaik AK. 2004. A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus. Virology 325:308 -319.
18. Halbur P, Bush E. 1997. Update on abortion storms and sow mortality. Swine Health Prod. 5:73.
19. Ni YY, Huang YW, Cao D, Opriessnig T, Meng XJ. 2011. Establishment of a DNA-launched infectious clone for a highly pneumovirulent strain of type 2 porcine reproductive and respiratory syndrome virus: identification and in vitro and in vivo characterization of a large spontaneous deletion in the nsp2 region. Virus Res. 160:264 -273.
20. Christopher-Hennings J, Nelson EA, Nelson JK, Benfield DA. 1997. Effects of a modified-live virus vaccine against porcine reproductive and respiratory syndrome in boars. Am. J. Vet. Res. 58:40-45.
21. Christopher-Hennings J, Dammen M, Nelson E, Rowland R, Oberst R. 2006. Comparison of RNA extraction methods for the detection of porcine reproductive and respiratory syndrome virus from boar semen. J. Virol. Methods 136:248 -253.
22. Reicks DL, Muñoz-Zanzi C, Mengeling W, Christopher-Hennings J, Lager K, Polson D, Dee S, Rossow K. 2006. Detection of porcine reproductive and respiratory syndrome virus in semen and serum of boars during the first six days after inoculation. Swine Health Prod. 14:35- 41.
23. Wasilk A, Callahan JD, Christopher-Hennings J, Gay TA, Fang Y, Dammen M, Reos ME, Torremorell M, Polson D, Mellencamp M, Nelson E, Nelson WM. 2004. Detection of U.S., Lelystad, and Europeanlike porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR. J. Clin. Microbiol. 42:4453- 4461.
24. Ramirez A, Wang C, Prickett JR, Pogranichniy R, Yoon KJ, Main R, Johnson JK, Rademacher C, Hoogland M, Hoffmann P, Kurtz A, Kurtz E, Zimmerman J. 2012. Efficient surveillance of pig populations using oral fluids. Prev. Vet. Med. 104:292-300.
25. Chittick WA, Stensland WR, Prickett JR, Strait EL, Harmon K, Yoon KJ, Wang C, Zimmerman JJ. 2011. Comparison of RNA extraction and RT-PCR methods for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) in porcine oral fluid specimens. J. Vet. Diagn. Invest. 23:248 -253.
26. Hermann JR, Munoz-Zanzi CA, Roof MB, Burkhart K, Zimmerman JJ. 2005. Probability of porcine reproductive and respiratory syndrome (PRRS) virus infection as a function of exposure route and dose. Vet. Microbiol. 110:7-16.
27. Landis JR, Koch GG. 1977. The measurement of observer agreement for categorical data. Biometrics 33:159 -174.