Microscale separation of heparosan, heparan sulfate, and heparin

Analytical Biochemistry

Volumn 434, Issue 2, 2013, Pages 215-217

  Zhao, Xue ^a,b,c   Yang, Bo ^b,c   Linkens, Kathryn ^c   Datta, Payel ^d   Onishi, Akihiro ^b   Zhang, Fuming ^b,d   Linhardt, Robert J ^b,c,d  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^c Rensselaer Polytechnic Institute   (United States)

^d RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Anion exchange chromatography; Disaccharide analysis; Glycosaminoglycan; Heparan sulfate; Heparin; Heparosan

Indexed keywords

CELL ENGINEERING; METABOLIC ENGINEERING; POLYSACCHARIDES; SEPARATION; SODIUM CHLORIDE; SULFUR COMPOUNDS;

ANION EXCHANGE CHROMATOGRAPHY; DISACCHARIDE ANALYSIS; GLYCOSAMINOGLYCANS; HEPARAN SULFATES; HEPARIN; HEPAROSAN;

CHROMATOGRAPHY;

DISACCHARIDE; GLYCOSAMINOGLYCAN; GLYCOSAMINOGLYCAN POLYSULFATE; HEPARAN SULFATE; HEPARIN; SODIUM CHLORIDE;

ANION EXCHANGE; ARTICLE; CHEMICAL STRUCTURE; DRUG ISOLATION; ENZYME SYNTHESIS; GLYCOBIOLOGY; MASS SPECTROMETRY; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SULFATION;

EID: 84872763655     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.12.009     Document Type: Article

References (12)

1. R.J. Linhardt Heparin: structure and activity J. Med. Chem. 46 2003 2551 2554
2. F. Zhang, Z.Q. Zhang, and R.J. Linhardt Glycosaminoglycans R.D. Cummings, M. Pierce, Handbook of Glycomics: Glycoconjugate Structural Analysis 2009 Academic Press/Elsevier San Diego (Chapter 3)
3. U. Lindahl, M. Kusche-Gullberg, and L. Kjellen Regulated diversity of heparan sulfate J. Biol. Chem. 273 1998 24979 24982
4. R.J. Linhardt, and J. Liu Synthetic heparin Curr. Opin. Pharmacol. 12 2012 217 219
5. J.D. Esko, and S.B. Selleck Order out of chaos: assembly of ligand binding sites in heparan sulfate Annu. Rev. Biochem. 71 2002 435 471
6. S. Peterson, A. Frick, and J. Liu Design of biologically active heparin sulfate and heparin using an enzyme-based approach Nat. Prod. Rep. 26 2009 610 627
7. J.Y. Baik, L. Gasimli, B. Yang, P. Datta, F.M. Zhang, C.A. Glasse, J.D. Esko, R.J. Linhardt, and S.T. Sharfstein Metabolic engineering of Chinese hamster ovary cells: towards a bioengineered heparin Metab. Eng. 14 2012 81 90
8. B. Yang, Y.C. Chang, A. Weyers, E. Sterner, and R.J. Linhardt Disaccharide analysis of glycosaminoglycan mixtures by ultra-performance liquid chromatography-mass spectrometry J. Chromatogr. A 1225 2012 91 98
9. B. Yang, A. Weyers, J.Y. Baik, E. Sterner, S. Sharfstein, S. Mousa, F. Zhang, J. Dordick, and R.J. Linhardt Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis Anal. Biochem. 415 2011 59 66
10. X. Zhao, B. Yang, P. Datta, L. Gasmili, F. Zhang, and R.J. Linhardt Cell-based microscale isolation of glycosaminoglycans for glycomics study J. Carbohydr. Chem. 31 2012 420 435
11. Z.Y. Wang, B. Yang, Z.Q. Zhang, M. Ly, M. Takieddin, S. Mousa, J. Liu, J.S. Dordick, and R.J. Linhardt Control of the heparosan N-deacetylation leads to an improved bioengineered heparin Appl. Microbiol. Biotechnol. 91 2011 91 99
12. M. Cesaretti, E. Luppi, F. Maccari, and N. Volpi A 96-well assay for uronic acid carbazole reaction Carbohydr. Polym. 54 2003 59 61