Phage lysin to control the overgrowth of normal flora in processed sputum samples for the rapid and sensitive detection of Mycobacterium tuberculosis by luciferase reporter phage assay

BMC Infectious Diseases

Volumn 13, Issue 1, 2013, Pages

  Subramanyam, Balaji ^a   Sivaramakrishnan, Gomathi ^a   Dusthackeer, Azger ^a   Kumar, Vanaja ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN TUBERCULOSIS   (India)

Author keywords

DLRPA; M. tuberculosis; Phage lysin; Rapid Diagnosis

Indexed keywords

ENZYME; LYSIN; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; BACTERIAL FLORA; BACTERIAL OVERGROWTH; BACTERIOPHAGE; BACTERIUM DETECTION; CONTAMINATION; CONTROLLED STUDY; CULTURE TECHNIQUE; DIAGNOSTIC LUCIFERASE REPORTER PHAGE ASSAY; INTERMETHOD COMPARISON; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; SENSITIVITY AND SPECIFICITY; SPUTUM;

BACTERIOLYSIS; BACTERIOPHAGES; HUMANS; LUCIFERASES; MUCOPROTEINS; MYCOBACTERIOPHAGES; MYCOBACTERIUM TUBERCULOSIS; SENSITIVITY AND SPECIFICITY; SPUTUM; TUBERCULOSIS;

EID: 84872762768     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-13-44     Document Type: Article

References (22)

1. Rosovitz MJ, Leppla SH. Virus deals anthrax a killer blow. Nature 2002, 418:825-826. 10.1038/418825a, 12192391.
2. Borysowski J, Weber-Dabrowska B, Gorski A. Bacteriophage endolysins as a novel class of antibacterial agents. Exp Biol Med (Maywood) 2006, 231:366-377.
3. Fischetti VA. Bacteriophage lysins as effective antibacterials. Curr Opin Microbiol 2008, 11:393-400. 10.1016/j.mib.2008.09.012, 2597892, 18824123.
4. O'Flaherty S, Ross RP, Coffey A. Bacteriophage and their lysins for elimination of infectious bacteria. FEMS Microbiol Rev 2009, 33:801-819. 10.1111/j.1574-6976.2009.00176.x, 19416364.
5. Kumar V, Balaji S, Gomathi NS, Venkataesan P, Gomathi S, Jayasankar K, Narayanan PR. Phage cocktail to control the exponential growth of normal flora in processed sputum specimens grown overnight in liquid medium for rapid TB diagnosis. J Microbiol Methods 2007, 68:536-542. 10.1016/j.mimet.2006.10.015, 17173989.
6. Subramanyam B, Kumar V, Perumal V, Nagamiah S. Phage lysin to supplement phagebiotics to decontaminate processed sputum specimens. Eur J Clin Microbiol Infect Dis 2010, 29:1407-1412. 10.1007/s10096-010-1018-8, 20652718.
7. Subramanyam B, Kumar V. Effect of bacteriophage lysin on lysogens. Asian Pacific J Tropical Med 2011, 4:306-308.
8. Subramanyam B, Sivaramakrishnan GN, Dusthackeer A, Nagamiah S, Kumar V. Phage lysin as a substitute for antibiotics to detect Mycobacterium tuberculosis from sputum samples with the BACTEC MGIT 960 system. Clin Microbiol Infect 2011, 18(5):497-501.
9. Selvakumar N, Sudhamathi S, Duraipandian M, Frieden TR, Narayanan PR. Reduced detection by Ziehl-Neelsen method of acid-fast bacilli in sputum samples preserved in cetylpyridinium chloride solution. Int J Tuberc Lung Dis 2004, 8:248-252.
10. Basnet R, Hinderaker SG, Enarson D, Malla P, Morkve O. Delay in the diagnosis of tuberculosis in Nepal. BMC Public Health 2009, 9:236. 10.1186/1471-2458-9-236, 2716339, 19602255.
11. Meintjes G, Schoeman H, Morroni C, Wilson D, Maartens G. Patient and provider delay in tuberculosis suspects from communities with a high HIV prevalence in South Africa: a cross-sectional study. BMC Infect Dis 2008, 8:72. 10.1186/1471-2334-8-72, 2413241, 18501019.
12. Greenaway C, Menzies D, Fanning A, Grewal R, Yuan L, FitzGerald JM. Delay in diagnosis among hospitalized patients with active tuberculosis-predictors and outcomes. Am J Respir Crit Care Med 2002, 165:927-933.
13. Hanna BA, Ebrahimzadeh A, Elliott LB. Multicenter evaluation of the BACTEC MGIT 960 system for recovery of mycobacteria. J Clin Microbiol 1999, 37:748-752. 84542, 9986844.
14. Rodrigues C, Shenai S, Sadani M. Evaluation of the bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high through put tertiary care centre. Indian J Med Microbiol 2009, 27:217-221. 10.4103/0255-0857.53203, 19584501.
15. Banaiee N, Bobadilla-Del-Valle M, Bardarov S. Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J Clin Microbiol 2001, 39:3883-3888. 10.1128/JCM.39.11.3883-3888.2001, 88459, 11682502.
16. Bardarov S, Dou H, Eisenach K. Detection and drug-susceptibility testing of M. tuberculosis from sputum samples using luciferase reporter phage: comparison with the Mycobacteria Growth Indicator Tube (MGIT) system. Diagn Microbiol Infect Dis 2003, 45:53-61. 10.1016/S0732-8893(02)00478-9, 12573551.
17. Jacobs WR, Barletta RG, Udani R. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science 1993, 260:819-822. 10.1126/science.8484123, 8484123.
18. Raparti V, Chitre T, Bothara K. Novel 4-(morpholin-4-yl)-N'-(arylidene)benzohydrazides: synthesis, antimycobacterial activity and QSAR investigations. Eur J Med Chem 2009, 44:3954-3960. 10.1016/j.ejmech.2009.04.023, 19464085.
19. Sivakumar PM, Prabhu Seenivasan S, Kumar V, Doble M. Novel 1,3,5-triphenyl-2-pyrazolines as anti-infective agents. Bioorg Med Chem Lett 2010, 20:3169-3172. 10.1016/j.bmcl.2010.03.083, 20385494.
20. Banaiee N, Bobadilla-del-Valle M, Riska PF. Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages. J Med Microbiol 2003, 52:557-561. 10.1099/jmm.0.05149-0, 12808076.
21. Whyte T, Hanahoe B, Collins T, Corbett-Feeney G, Cormican M. Evaluation of the BACTEC MGIT 960 and MB BAC/T systems for routine detection of Mycobacterium tuberculosis. J Clin Microbiol 2000, 38:3131-3132. 87212, 11001663.
22. Tortoli E, Cichero P, Piersimoni C, Simonetti MT, Gesu G, Nista D. Use of BACTEC MGIT 960 for recovery of mycobacteria from clinical specimens: multicenter study. J Clin Microbiol 1999, 37:3578-3582. 85696, 10523555.