Functional multiplex reporter assay using tagged Gaussia luciferase

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Van Rijn, Sjoerd ^a   Nilsson, Jonas ^a,b   Noske, David P ^a   Peter Vandertop, W ^a   Tannous, Bakhos A ^c   Würdinger, Thomas ^a,c  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UMEÅ UNIVERSITY   (Sweden)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; LUCIFERASE; PHOTOPROTEIN; RED FLUORESCENT PROTEIN;

ANIMAL; ARTICLE; COPEPOD; ENZYMOLOGY; FLUORESCENCE MICROSCOPY; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; MOUSE; NUDE MOUSE; REPORTER GENE; TUMOR CELL LINE; XENOGRAFT;

ANIMALS; CELL LINE, TUMOR; COPEPODA; EPITOPES; GENES, REPORTER; HUMANS; LUCIFERASES; LUMINESCENT PROTEINS; MICE; MICE, NUDE; MICROSCOPY, FLUORESCENCE; TRANSPLANTATION, HETEROLOGOUS;

MLCS; MLOWN;

EID: 84872715196     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep01046     Document Type: Article

References (17)

1. Gross, S. & Piwnica-Worms, D. Spying on cancer: molecular imaging in vivo with genetically encoded reporters. Cancer cell 7, 5-15 (2005). (Pubitemid 40126378)
2. Pittet, M. J. & Weissleder, R. Intravital Imaging. Cell 147, 983-991 (2011).
3. Contag, C. H. & Ross, B. D. It's not just about anatomy: in vivo bioluminescence imaging as an eyepiece into biology. Journal of magnetic resonance imaging/: JMRI 16, 378-87 (2002). (Pubitemid 35167873)
4. Bhaumik, S. & Gambhir, S. S. Optical imaging of Renilla luciferase reporter gene expression in living mice. Proceedings of the National Academy of Sciences of the United States of America 99, 377-82 (2002). (Pubitemid 34060369)
5. Tannous, B. A., Kim, D.-E., Fernandez, J. L., Weissleder, R. & Breakefield, X. O. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Molecular therapy/: the journal of the American Society of Gene Therapy 11, 435-43 (2005). (Pubitemid 40277320)
6. Wurdinger, T. et al. A secreted luciferase for ex vivo monitoring of in vivo processes. Nature methods 5, 171-3 (2008). (Pubitemid 351181745)
7. Bhang, H., Eun C., Gabrielson, K. L., Laterra, J., Fisher, P. B. & Pomper, M. G. Tumor-specific imaging through progression elevated gene-3 promoter-driven gene expression. Nature medicine 17, 123-9 (2011).
8. Badr, C. E., Hewett, J. W., Breakefield, X. O. & Tannous, B. A. A highly sensitive assay for monitoring the secretory pathway and ER stress. PloS one 2, e571 (2007).
9. Lee, J. Y. et al. Development of a dual-luciferase reporter system for in vivo visualization ofMicroRNA biogenesis and posttranscriptional regulation. Journal of nuclear medicine/: official publication, Society of Nuclear Medicine 49, 285-94 (2008).
10. Tian, W. et al. High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays. PloS one 7, e29551 (2012).
11. McMillin, D. W. et al. Tumor cell-specific bioluminescence platform to identify stroma-induced changes to anticancer drug activity. Nature medicine 16, 483-9 (2010).
12. Badr, C. E. & Tannous, B. A. Bioluminescence imaging: progress and applications. Trends in Biotechnology 29, 624-33 (2011).
13. Shaner, N. C., Steinbach, P. A. & Tsien, R. Y. A guide to choosing fluorescent proteins. Nature methods 2, 905-9 (2005). (Pubitemid 43108726)
14. Filonov, G. S. et al. Bright and stable near-infrared fluorescent protein for in vivo imaging. Nature biotechnology 29, 757-61 (2011).
15. Deliolanis, N. C. et al. In vivo tomographic imaging of red-shifted fluorescent proteins. Biomedical optics express 2, 887-900 (2011).
16. Tannous, B. a Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nature protocols 4, 582-91 (2009).
17. Niers, J. M., Kerami, M., Pike, L., Lewandrowski, G. & Tannous, B. A. Multimodal in vivo imaging and blood monitoring of intrinsic and extrinsic apoptosis. Molecular therapy/: the journal of the American Society of Gene Therapy 19, 1090-6 (2011).