Validation of a Poisson-distributed limiting dilution assay (LDA) for a rapid and accurate resolution of multiclonal infections in natural Trypanosoma cruzi populations

Journal of Microbiological Methods

Volumn 92, Issue 2, 2013, Pages 220-225

  Ramírez, Juan David ^a   Herrera, Claudia ^a   Bogotá, Yizeth ^a   Duque, María Clara ^a   Suárez Rivillas, Alejandro ^a   Guhl, Felipe ^a  

^a UNIVERSIDAD DE LOS ANDES   (Colombia)

Author keywords

Aneuploidy; Chagas disease; Infrapopulation; Multiclonality; Superinfection; Trypanosoma cruzi

Indexed keywords

ANEUPLOIDY; ARTICLE; ASSAY; CHAGAS DISEASE; CLONAL VARIATION; CONTROLLED STUDY; FEASIBILITY STUDY; GENE LOCUS; GENOTYPE; INTERMETHOD COMPARISON; LIMITING DILUTION ASSAY; MICROSATELLITE MARKER; NONHUMAN; PARASITE ISOLATION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; RELIABILITY; TRYPANOSOMA CRUZI;

CHAGAS DISEASE; CLINICAL LABORATORY TECHNIQUES; COINFECTION; DNA, RIBOSOMAL; EXONS; GENOTYPE; HUMANS; MICROSATELLITE REPEATS; PARASITOLOGY; RNA, SPLICED LEADER; TRYPANOSOMA CRUZI;

DASYPUS NOVEMCINCTUS; DIDELPHIS MARSUPIALIS; HEXAPODA; HOMO SAPIENS; MAMMALIA; PANSTRONGYLUS GENICULATUS; PROTISTA; RATTUS RATTUS; RHODNIUS PROLIXUS; TAMANDUA TETRADACTYLA; TRIATOMA VENOSA; TRYPANOSOMA CRUZI;

EID: 84872707282     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.11.002     Document Type: Article

References (35)

1. Butterworth A.S., Robertson A.J., Mei-Fong H., Gatton M.L., McCarthy J.S., Trenholme K.R. An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites. Malar. J. 2011, 10:95.
2. Duque M., Ramírez J.D., Rendón L., Guhl F. Evaluación de la variabilidad genética de aislamientos colombianos de Trypanosoma cruzi I mediante marcadores microsatélites. Infection 2011, 15.
3. Frisan T., Levitsky V., Masucci M. Limiting Dilution Assay Epstein-Barr Virus Protocols 2001, 213-216. Humana Press. J.B. Wilson, G.H.W. May (Eds.).
4. Goldberg S.S., Chiari E. Growth and isolation of single colonies of Trypanosoma cruzi on solid medium. J. Parasitol. 1980, 66:677-679.
5. Gomes M.L., Araujo S.M., Chiari E. Trypanosoma cruzi: growth of clones on solid medium using culture and blood forms. Mem. Inst. Oswaldo Cruz 1991, 86:131-132.
6. Hamilton P.B., Teixeira M.G., Stevens J.R. The evolution of Trypanosoma cruzi: the 'bat seeding' hypothesis. Trends Parasitol. 2012, 28:136-141.
7. Lewis M.D., Llewellyn M.S., Yeo M., Acosta N., Gaunt M.W., Miles M.A. Recent, independent and anthropogenic origins of Trypanosoma cruzi hybrids. PLoS Negl. Trop. Dis. 2011, 5:e1363.
8. Llewellyn M.S., Rivett-Carnac J.B., Fitzpatrick S., Lewis M.D., Yeo M., Gaunt M.W., Miles M.A. Extraordinary Trypanosoma cruzi diversity within single mammalian reservoir hosts implies a mechanism of diversifying selection. Int. J. Parasitol. 2011, 41:609-614.
9. Lyko B., Hammershaimb E., Nguitragool W., Wellems T., Desai S. A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplates. Malar. J. 2012, 11:124.
10. Macedo A.M., Machado C.R., Oliveira R.P., Pena Sr.D.J. Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of chagas disease. Mem. Inst. Oswaldo Cruz 2004, 99:1-12.
11. Macedo A., Segatto M. Implications of Trypanosoma cruzi intraspecific diversity in the pathogenesis of Chagas disease. American Trypanosomiasis 2010, 489-522.
12. Manoel-Caetano F.D., Carareto C.M., Borim A.A., Miyazaki K., Silva A.E. kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients. Trans. R. Soc. Trop. Med. Hyg. 2008, 102:1102-1107.
13. Mejía-Jaramillo A.M., Peña V.H., Triana-Chávez O. Trypanosoma cruzi: biological characterization of lineages I and II supports the predominance of lineage I in Colombia. Exp. Parasitol. 2009, 121:83-91.
14. Messenger L.A., Llewellyn M.S., Bhattacharyya T., Franzén O., Lewis M.D., Ramírez J.D., Carrasco H.J., Andersson B., Miles M.A. Multiple mitochondrial introgression events and heteroplasmy in Trypanosoma cruzi revealed by maxicircle MLST and next generation sequencing. PLoS Negl. Trop. Dis. 2012, 6:e1584.
15. Miles M.A., Povoa M.M., de Souza A.A., Lainson R., Shaw J.J., Ketteridge D.S. Chagas's disease in the Amazon Basin: the distribution of Trypanosoma cruzi zymodemes 1 and 3 in Para State, north Brazil. Trans. R. Soc. Trop. Med. Hyg. 1981, 75:667-674.
16. Mondragon A., Wilkinson S.R., Taylor M.C., Kelly J.M. Optimization of conditions for growth of wild-type and genetically transformed Trypanosoma cruzi on agarose plates. Parasitology 1999, 118:461-467.
17. Ng-Ying-Kin N.M., Yaphe W. Properties of agar: parameters affecting gel-formation and the agarone-iodine reaction. Carbohydr. Res. 1972, 25:379-385.
18. Llewellyn M.S., Miles M.A., Carrasco H.J., Lewis M.D., Yeo M. Genome-scale multilocus microsatellite typing of Trypanosoma cruzi discrete typing unit I reveals phylogeographic structire and specific genotypes linked to human infection. PLoS Pathog. 2009, 5:e1000410.
19. Portugal S., Carret C., Recker M., Armitage A., Gonzalves L., Epiphanio S., Sullivan D., Roy C., Newbold C., Drakesmith H., Mota M. Host-mediated regulation of superinfection in malaria. Nat. Med. 2011, 17:732-737.
20. Ramírez J.D., Guhl F., Rendón L., Rosas F., Marin-Neto A., Morillo C. Chagas cardiomyopathy and Trypanosoma cruzi genotypes circulating in chronic chagasic patients. PLoS Negl.Trop. Dis. 2010, 4:e899.
21. Ramírez J.D., Guhl F., Messenger L.A., Lewis M.D., Montilla M., Cucunuba Z., Miles M.A., Llewellyn M.S. Contemporary cryptic sexuality in Trypanosoma cruzi. Mol Ecol. 2012, 21(17):4216-4226.
22. Rassi A., Rassi A., Marcondes de Rezende J. American Trypanosomiasis (Chagas Disease). Infect. Dis. Clin. N. Am. 2012, 26:275-291.
23. Real F., Mortara R.A., Rabinovitch M. Fusion between Leishmania amazonensis and Leishmania major parasitophorous vacuoles: live imaging of coinfected macrophages. PLoS Negl. Trop. Dis. 2010, 4:e905.
24. Santos W.G., Metcheva I., Buck G.A. Colony polymerase chain reaction of stably transfected Trypanosoma cruzi grown on solid medium. Mem. Inst. Oswaldo Cruz 2000, 95:111-114.
25. Tibayrenc M., Ayala F.J. Reproductive clonality of pathogens: a perspective on pathogenic viruses, bacteria, fungi, and parasitic protozoa. Proc Natl. Acad. Sci. U.S.A 2012, 2012 Sep 4. [Epub ahead of print].
26. Trager W., Tershakovec M., Lyandvert L., Stanley H., Lanners N., Gubert E. Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, cloroquine sensitivity, and formation of gametocytes. Proc. Natl. Acad. Sci. 1981, 78:6527-6530.
27. Valadares H.M.S., Pimenta J.R., de Freitas J.M., Duffy T., Bartholomeu D.C., de Paula Oliveira R., Chiari E., Moreira M.C.V., Filho G.B., Schijman A.G., Franco G.R., Machado C.R., Pena S.D.J., Macedo A.M. Genetic profiling of Trypanosoma cruzi directly in infected tissues using nested PCR of polymorphic microsatellites. Int. J. Parasitol. 2008, 38:839-850.
28. Vago A.R., Andrade L.O., Leite A.A., dD́Avila D., Macedo A. Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease. Am. J. Pathol. 2000, 156:1805-1809.
29. Valadares H.M.S., Pimenta J.R., Segatto M., Veloso V.M., Gomes M.L., Chiari E., Gollob K.J., Bahia M.T., de Lana M., Franco G.R., Machado C.R., Pena S.D.J., Macedo A.M. Unequivocal identification of subpopulations in putative multiclonal Trypanosoma cruzi strains by FACs single cell sorting and genotyping. PLoS Negl. Trop. Dis. 2012, 6:e1722.
30. Venegas J., Coñoepan W., Pichuantes S., Miranda S., Apt W. Differential distribution of Trypanosoma cruzi clones in human chronic chagasic cardiopathic and non-cardiopathic individuals. Acta Trop. 2009, 109:187-193.
31. Venegas J., Miranda S., Coñoepan W., Pichuantes S., Jercic M.I., González C., Gajardo M., Apt W., Arribada A., Sánchez G. Microsatellite marker analysis shows differentiation among Trypanosoma cruzi populations of peripheral blood and dejections of Triatoma infestans fed on the same chronic chagasic patient. Parasitol. Res. 2010, 107:855-863.
32. World Health Organization (WHO) Special Programme for Research and Training in Tropical Diseases (TDR) Report of Scientific Group in Chagas Disease. Buenos Aires, Argentina, April 17-20, 2005 2007, (Update July 2007).
33. Yeo M., Lewis M.D., Carrasco H.J., Acosta N., Llewellyn M., da Silva Valente S.A., de Costa Valente V., de Arias A.R., Miles M.A. Resolution of multiclonal infections of Trypanosoma cruzi from naturally infected triatomine bugs and from experimentally infected mice by direct plating on a sensitive solid medium. Int. J. Parasitol. 2007, 37:111-120.
34. Zingales B., Andrade S., Briones M., Campbell D., Chiari E., Fernandes O., Guhl F., Lages-Silva E., Macedo A., Machado C., Miles M., Romanha A., Sturm N., Tibayrenc M., Schijman A. A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem. Inst. Oswaldo Cruz 2009, 104:1051-1054.
35. Zingales B., Miles M.A., Campbell D.A., Tibayrenc M., Macedo A.M., Teixeira M.M.G., Schijman A.G., Llewellyn M.S., Lages-Silva E., Machado C.R., Andrade S.G., Sturm N.R. The revised Trypanosoma cruzi subspecific nomenclature: rationale, epidemiological relevance and research applications. Inf. Genet. Evol. 2012, 12:240-253.