Comparison of the new Abbott Real Time CMV assay and the Abbott CMV PCR Kit for the quantitation of plasma cytomegalovirus DNAemia

Diagnostic Microbiology and Infectious Disease

Volumn 75, Issue 2, 2013, Pages 207-209

  Clari, María Ángeles ^a   Bravo, Dayana ^a   Costa, Elisa ^a   Muñoz Cobo, Beatriz ^a   Solano, Carlos ^a   Remigia, María José ^a   Giménez, Estela ^a   BenMarzouk Hidalgo, Omar J ^b   Pérez Romero, Pilar ^b   Navarro, David ^a,c  

^a HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

^b HOSPITAL UNIVERSITARIO VIRGEN DEL ROCÍO   (Spain)

^c UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Abbott PCR assays; Cytomegalovirus; Plasma CMV DNAemia; Real time PCR

Indexed keywords

VIRUS DNA;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; CYTOMEGALOVIRUS; DIAGNOSTIC KIT; DNA DETERMINATION; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; VIRUS LOAD;

CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; HUMANS; LINEAR MODELS; POLYMERASE CHAIN REACTION;

EID: 84872490622     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.10.010     Document Type: Article

References (9)

1. Bland J.M., Altman D.G. Statistical method for assessing the agreement between two methods of clinical measurement. Lancet 1986, i:307-310.
2. Boom R., Sol C.J., Schuurman T., Van Breda A., Weel J.F., Beld M., et al. Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented. J Clin Microbiol 2002, 40:4105-4113.
3. Bravo D., Clari M.A., Costa E., Costa Muñoz-Cobo B., Solano C., Remigia M.J., et al. Comparative evaluation of three automated systems for DNA extraction in conjunction with three commercially available real-time PCR assays for quantitation of plasma Cytomegalovirus DNAemia in allogeneic stem cell transplant recipients. J Clin Microbiol 2011, 49:2899-2904.
4. Caliendo A.M., Ingersoll J., Fox-Canale A.M., Pargman S., Bythwood T., Hayden M.K., et al. Evaluation of real-time PCR laboratory- developed tests using analyte-specific reagents for cytomegalovirus quantification. J Clin Microbiol 2007, 45:1723-1737.
5. Fryer JF, Heath AB, Anderson R, Minor PD, and the collaborative study group. Collaborative study to evaluate the proposed 1st WHO International Standard for human cytomegalovirus (HCMV) for nucleic acid amplification (NAT)-based assays. WHO ECBS Report 2010; WHO/BS/10.2138.
6. Gimeno C., Solano C., Latorre J.C., Hernández-Boluda J.C., Clari M.A., Remigia M.J., et al. Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of pre-emptive therapy for allogeneic hematopoietic stem cell transplant recipients. J Clin Microbiol 2008, 46:3311-3318.
7. Gracia-Ahufinger I., Tormo N., Espigado I., Solano C., Urbano-Ispizua A., Clari M.A., et al. Differences in cytomegalovirus plasma viral loads measured in allogeneic hematopoietic stem cell transplant recipients using two commercial real-time PCR assays. J Clin Virol 2010, 48:142-146.
8. Pang X.L., Fox J.D., Fenton J.M., Miller G.G., Caliendo A.M., Preiksaitis J.M. Interlaboratory comparison of cytomegalovirus viral load assays. Am J Transplant 2009, 9:258-268. American Society of Transplantation Infectious Diseases Community of Practice, Canadian Society of TransplantationCanadian Society of Transplantation.
9. Solano C., Navarro D. Clinical virology of cytomegalovirus infection following hematopoietic transplantation. Future Virol 2010, 5:111-124.