Solvent selection for insoluble ligands, a challenge for biological assay development: A TNF-α/SPD304 study

ACS Medicinal Chemistry Letters

Volumn 4, Issue 1, 2013, Pages 137-141

  Papaneophytou, Christos P ^a   Mettou, Anthi K ^a,b   Rinotas, Vagelis ^c,d   Douni, Eleni ^c,d   Kontopidis, George A ^a,b  

^a Centre for Research and Technology of Thessaly (CERETETH)   (Greece)

^b UNIVERSITY OF THESSALY   (Greece)

^c AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

^d BIOMEDICAL SCIENCES RESEARCH CENTER ALEXANDER FLEMING   (Greece)

Author keywords

aqueous solubility; fluorescence binding assay; insoluble ligands; protein stability; solvent selection

Indexed keywords

DIMETHYL SULFOXIDE; GLYCEROL; MACROGOL 3350; PROTEIN INHIBITOR; SPD 304; TUMOR NECROSIS FACTOR ALPHA INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; BINDING ASSAY; FLUORESCENCE ANALYSIS; PRIORITY JOURNAL; PROTEIN STABILITY;

EID: 84872350030     PISSN: None     EISSN: 19485875     Source Type: Journal    
DOI: 10.1021/ml300380h     Document Type: Article

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