A new, rapid and reproducible method to obtain high quality endothelium in vitro

Cytotechnology

Volumn 65, Issue 1, 2013, Pages 1-14

  Jiménez, Nuria ^a   Krouwer, Vincent J D ^a   Post, Jan A ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Cobblestone; Electron microscopy; Endothelium; HUVEC; Immuno fluorescence; LDL uptake; Umbilical vein

Indexed keywords

FIBRONECTIN; GELATIN;

ARTICLE; CELL ISOLATION; CELL MATURATION; CHEMICAL REACTION; CLINICAL ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; EXTRACELLULAR MATRIX; FEMALE; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; INTERNALIZATION; MONOLAYER CULTURE; PHASE CONTRAST MICROSCOPY; QUALITATIVE ANALYSIS; REPRODUCIBILITY; ROOM TEMPERATURE; TRYPSINIZATION; UMBILICAL VEIN ENDOTHELIAL CELL; VALIDATION PROCESS;

EID: 84872333070     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-012-9459-9     Document Type: Article

References (49)

1. Akers CK, Dardik I, Dardik H, Wodka M (1977) Computational methods comparing the surface properties of the inner walls of isolated human veins and synthetic biomaterials. J Colloid Interface Sci 59:461
2. Albelda SM, Daise M, Levine EM, Buck CA (1989) Identification and characterization of cell-substratum adhesion receptors on cultured human endothelial cells. J Clin Invest 83:1992-2002
3. Anamelechi CC, Clermont EC, Novak MT, Reichert WM (2009) Dynamic seeding of perfusing human umbilical vein endothelial cells (HUVECs) onto dual-function cell adhesion ligands: Arg-Gly-Asp (RGD)-streptavidin and biotinylated fibronectin. Langmuir 25:5725-5730
4. Baudin B, Bruneel A, Bosselut N, Vaubourdolle M (2007) A protocol for isolation and culture of human umbilical vein endothelial cells. Nat Protoc 2:481-485
5. Bazzoni G, Lampugnani MG, Martinez-Estrada OM, Dejana E (2002) Analysis of intercellular adhesion molecules in endothelial cells. In: Fleming TP (ed) Cell-Cell interactions. Practical approach, 2nd edn. Oxford University Press, New York, pp 37-46
6. Chazov EI, Alexeev AV, Antonov AS, Koteliansky VE, Leytin VL, Lyubimova EV, Repin VS, Sviridov DD, Torchilin VP, Smirnov VN (1981) Endothelial cell culture on fibrillar collagen: model to study platelet adhesion and liposome targeting to intercellular collagen matrix. Proc Natl Acad Sci USA 78:5603-5607
7. Clark RA, Folkvord JM, Nielsen LD (1986) Either exogenous or endogenous fibronectin can promote adherence of human endothelial cells. J Cell Sci 82:263-280
8. Davis J, Crampton S, Hughes C (2007) Isolation of Human Umbilical Vein Endothelial Cells (HUVEC). J Vis Exp (3):e183. doi: 10.3791/183
9. Dejana E (2004) Endothelial cell-cell junctions: happy together. Nat Rev Mol Cell Biol 5:261-270
10. Elgjo RF, Henriksen T, Evensen SA (1975) Ultrastructural identification of umbilical cord vein endothelium in situ and in culture. Cell Tissue Res 162:49-59
11. Franke RP, Grafe M, Schnittler H, Seiffge D, Mittermayer C, Drenckhahn D (1984) Induction of human vascular endothelial stress fibres by fluid shear stress. Nature 307:648-649
12. Gimbrone MA Jr, Cotran RS, Folkman J (1974) Human vascular endothelial cells in culture. Growth and DNA synthesis. J Cell Biol 60:673-684
13. Grant DS, Kleinman HK, Martin GR (1990) The role of basement membranes in vascular development. Ann N Y Acad Sci 588:61-72
14. Hasegawa N, Yamamoto M, Imamura T, Mitsui Y, Yamamoto K (1988) Evaluation of long-term cultured endothelial cells as a model system for studying vascular ageing. Mech Ageing Dev 46:111-123
15. Jaffe EA, Mosher DF (1978) Synthesis of fibronectin by cultured human endothelial cells. J Exp Med 147:1779-1791
16. Jaffe EA, Hoyer LW, Nachman RL (1973a) Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest 52:2757-2764
17. Jaffe EA, Nachman RL, Becker CG, Minick CR (1973b) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52:2745-2756
18. Jaffe EA, Hoyer LW, Nachman RL (1974) Synthesis of von Willebrand factor by cultured human endothelial cells. Proc Natl Acad Sci USA 71:1906-1909
19. Jaffe EA, Minick CR, Adelman B, Becker CG, Nachman R (1976) Synthesis of basement membrane collagen by cultured human endothelial cells. J Exp Med 144:209-225
20. Jiménez N, Post JA (2012) A novel approach for intracellular 3D immuno-labeling for electron tomography. Traffic. doi: 10.1111/j.1600-0854.2012. 01363.x
21. Jiménez N, Humbel BM, van Donselaar E, Verkleij AJ, Burger KN (2006) Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayers. J Microsc 221:216-223
22. Jiménez N, Vocking K, van Donselaar EG, Humbel BM, Post JA, Verkleij AJ (2009) Tannic acid-mediated osmium impregnation after freeze-substitution: a strategy to enhance membrane contrast for electron tomography. J Struct Biol 166:103-106
23. Jiménez N, Van Donselaar EG, De Winter DA, Vocking K, Verkleij AJ, Post JA (2010) Gridded Aclar: preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB-SEM. J Microsc 237:208-220
24. Kiyonaga H, Doi Y, Karasaki Y, Arashidani K, Itoh H, Fujimoto S (2001) Expressions of endothelin-1, fibronectin, and interleukin-1alpha of human umbilical vein endothelial cells under prolonged culture. Med Electron Microsc 34:41-53
25. Kleinman HK, McGarvey ML, Liotta LA, Robey PG, Tryggvason K, Martin GR (1982) Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21:6188-6193
26. Laurens N, van Hinsbergh VW (2004) Isolation, purification and culture of human micro- and macrovascular endothelial cells. In: Augustin H (ed) Methods in endothelial cell biology. Springer Lab Manual, Springer, Berlin, Heidelberg, pp 3-13
27. Lawley TJ, Kubota Y (1989) Induction of morphologic differentiation of endothelial cells in culture. J Invest Dermatol 93:59S-61S
28. Lebbink MN, Jiménez N, Vocking K, Hekking LH, Verkleij AJ, Post JA (2010) Spiral coating of the endothelial caveolar membranes as revealed by electron tomography and template matching. Traffic 11:138-150
29. Levene CI, Bartlet CP, Heale G (1988) Identification of the connective tissues synthesized by the venous and arterial endothelia of the human umbilical cord: a comparative study. Br J Exp Pathol 69:177-188
30. Lewis LJ, Hoak JC, Maca RD, Fry GL (1973) Replication of human endothelial cells in culture. Science 181:453-454
31. Li WC, Ruan XZ, Zhang HM, Zeng YJ (2006) Biomechanical properties of different segments of human umbilical cord vein and its value for clinical application. J Biomed Mater Res B Appl Biomater 76:93-97
32. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
33. Macarak EJ, Howard PS (1983) Adhesion of endothelial cells to extracellular matrix proteins. J Cell Physiol 116:76-86
34. Maciag T, Hoover GA, Stemerman MB, Weinstein R (1981) Serial propagation of human endothelial cells in vitro. J Cell Biol 91:420-426
35. Manconi F, Markham R, Fraser IS (2000) Culturing endothelial cells of microvascular origin. Methods Cell Sci 22:89-99
36. Marin V, Kaplanski G, Gres S, Farnarier C, Bongrand P (2001) Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells. J Immunol Methods 254:183-190
37. Martins-Green M, Petreaca M, Yao M (2008) An assay system for in vitro detection of permeability in human "endothelium". Methods Enzymol 443:137-153
38. Maruyama Y (1963) The human endothelial cell in tissue culture. Z Zellforsch Mikrosk Anat 60:69-79
39. Nachman RL, Jaffe EA (2004) Endothelial cell culture: beginnings of modern vascular biology. J Clin Invest 114:1037-1040
40. Pareti FI, Fujimura Y, Dent JA, Holland LZ, Zimmerman TS, Ruggeri ZM (1986) Isolation and characterization of a collagen binding domain in human von Willebrand factor. J Biol Chem 261:15310-15315
41. Redgrave TG, Roberts DC, West CE (1975) Separation of plasma lipoproteins by density-gradient ultracentrifugation. Anal Biochem 65:42-49
42. Reichlin T, Wild A, Durrenberger M, Daniels AU, Aebi U, Hunziker PR, Stolz M (2005) Investigating native coronary artery endothelium in situ and in cell culture by scanning force microscopy. J Struct Biol 152:52-63
43. Simionescu M, Antohe F (2006) Functional ultrastructure of the vascular endothelium: changes in various pathologies. Handb Exp Pharmacol 176:41-69
44. Smeets EF, von Asmuth EJ, van der Linden CJ, Leeuwenberg JF, Buurman WA (1992) A comparison of substrates for human umbilical vein endothelial cell culture. Biotech Histochem 67:241-250
45. Striker GE, Harlan JM, Schwartz SM (1980) Human endothelial cells in vitro. Methods Cell Biol 21A:135-151
46. Valentijn KM, van Driel LF, Mourik MJ, Hendriks GJ, Arends TJ, Koster AJ, Valentijn JA (2010) Multigranular exocytosis of Weibel-Palade bodies in vascular endothelial cells. Blood 116:1807-1816
47. Vasile E, Simionescu M, Simionescu N (1983) Visualization of the binding, endocytosis, and transcytosis of low-density lipoprotein in the arterial endothelium in situ. J Cell Biol 96:1677-1689
48. Wagner DD, Olmsted JB, Marder VJ (1982) Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells. J Cell Biol 95:355-360
49. Weibel ER, Palade GE (1964) New cytoplasmic components in arterial endothelia. J Cell Biol 23:101-112