Enhancement of osteogenic and chondrogenic differentiation of human embryonic stem cells by mesodermal lineage induction with BMP-4 and FGF2 treatment

Biochemical and Biophysical Research Communications

Volumn 430, Issue 2, 2013, Pages 793-797

  Lee, Tae Jin ^a,b   Jang, Jiho ^c   Kang, Seokyung ^b   Jin, Min ^b   Shin, Heungsoo ^a   Kim, Dong Wook ^c   Kim, Byung Soo ^b  

^a Hanyang University   (South Korea)

^b Seoul National University   (South Korea)

^c Yonsei University College of Medicine   (South Korea)

Author keywords

BMP 4; Chondrogenic differentiation; FGF2; Human embryonic stem cells; Mesodermal differentiation; Osteogenic differentiation

Indexed keywords

BONE MORPHOGENETIC PROTEIN 4; FIBROBLAST GROWTH FACTOR 2;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; CELL CULTURE; CELL DIFFERENTIATION; CELL LINEAGE; CHONDROGENESIS; CONTROLLED STUDY; EMBRYONIC STEM CELL; GENE EXPRESSION; HUMAN; HUMAN CELL; MESODERM; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BONE MORPHOGENETIC PROTEIN 4; CELL DIFFERENTIATION; CELL LINEAGE; CELLS, CULTURED; CHONDROGENESIS; EMBRYOID BODIES; EMBRYONIC STEM CELLS; FIBROBLAST GROWTH FACTOR 2; HUMANS; MESODERM; OSTEOGENESIS;

EID: 84872280573     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.11.067     Document Type: Article

References (33)

1. Itskovitz-Eldor J., Schuldiner M., Karsenti D., et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med. 2000, 6:88-95.
2. Keller G.M. In vitro differentiation of embryonic stem cells. Curr. Opin. Cell Biol. 1995, 7:862-869.
3. Höpfl G., Gassmann M., Desbaillets I. Differentiating embryonic stem cells into embryoid bodies. Methods Mol. Biol. 2004, 254:79-98.
4. Hwang Y.S., Randle W.L., Bielby R.C., et al. Enhanced derivation of osteogenic cells from murine embryonic stem cells after treatment with HepG2-conditioned medium and modulation of the embryoid body formation period: application to skeletal tissue engineering. Tissue Eng. 2006, 12:1381-1392.
5. Kitagawa M., Era T. Differentiation of mesodermal cells from pluripotent stem cells. Int. J. Hematol. 2010, 91:373-383.
6. Zhang P., Li J., Tan Z., et al. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood 2008, 111:1933-1941.
7. Yang L., Soonpaa M.H., Adleret E.D., et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 2008, 453:524-528.
8. Yook J.Y., Kim M.J., Son M.J., et al. Combinatorial activin receptor-like kinase/Smad and basic fibroblast growth factor signals stimulate the differentiation of human embryonic stem cells into the cardiac lineage. Stem Cells Dev. 2011, 20:1479-1490.
9. Yu P., Pan G., Yu J., et al. FGF2 sustains NANOG and switches the outcome of BMP4-induced human embryonic stem cell differentiation. Cell Stem Cell 2011, 8:326-334.
10. Winnier G., Blessing M., Labosky P.A., et al. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev. 1995, 9:2105-2116.
11. Beppu H., Kawabata M., Hamamoto T., et al. BMP type II receptor is required for gastrulation and early development of mouse embryos. Dev. Biol. 2000, 221:249-258.
12. Mishina Y., Suzuki A., Uenoet N., et al. Bmpr encodes a type I bone morphogenetic protein receptor that is essential for gastrulation during mouse embryogenesis. Genes Dev. 1995, 9:3027-3037.
13. Wiles M.V., Johansson B.M. Embryonic stem cell development in a chemically defined medium. Exp. Cell Res. 1999, 247:241-248.
14. Czyz J., Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. Differentiation 2001, 68:167-174.
15. Park C., Afrikanova I., Chung Y.S., et al. A hierarchical order of factors in the generation of FLK1- and SCL-expressing hematopoietic and endothelial progenitors from embryonic stem cells. Development 2004, 131:2749-2762.
16. Yang X., Karsenty G. Transcription factors in bone: developmental and pathological aspects. Trends Mol. Med. 2002, 8:340-345.
17. Malaval L., Modrowski D., Gupta A.K., et al. Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures. J. Cell. Physiol. 1994, 158:555-572.
18. Akiyama H., Chaboissier M.C., Martin J.F., et al. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev. 2002, 16:2813-2828.
19. Bell D.M., Leung K.K., Wheatley S.C., et al. SOX9 directly regulates the type-II collagen gene. Nat. Genet. 1997, 16:174-178.
20. Vogel G. Cell biology. Ready or not? human ES cells head toward the clinic. Science 2005, 308:1534-1538.
21. Prokhorova T.A., Harkness L.M., Frandsen U., et al. Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. Stem Cells Dev. 2009, 18:47-54.
22. Hentze H., Graichen R., Colman A. Cell therapy and the safety of embryonic stem cell-derived grafts. Trends Biotechnol. 2007, 25:24-32.
23. Zandstra P.W., Bauwens C., Yin T., et al. Scalable production of embryonic stem cell-derived cardiomyocytes. Tissue Eng. 2003, 9:767-778.
24. Cao F., Lin S., Xie X., et al. In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery. Circulation 2006, 113:1005-1014.
25. Schuldiner M., Itskovitz-Eldor J., Benvenisty N. Selective ablation of human embryonic stem cells expressing a " suicide" gene. Stem Cells 2003, 21:257-265.
26. Handgretinger R., Lang P., Ihm K., et al. Isolation and transplantation of highly purified autologous peripheral CD34(+) progenitor cells: purging efficacy, hematopoietic reconstitution and long-term outcome in children with high-risk neuroblastoma. Bone Marrow Transplant 2002, 29:731-736.
27. Shibata H., Ageyama N., Tanaka Y., et al. Improved safety of hematopoietic transplantation with monkey embryonic stem cells in the allogeneic setting. Stem Cells 2006, 24:1450-1457.
28. Xu C., Police S., Rao N., et al. Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ. Res. 2002, 91:501-508.
29. Kumashiro Y., Asahina K., Ozeki R., et al. Enrichment of hepatocytes differentiated from mouse embryonic stem cells as a transplantable source. Transplantation 2005, 79:550-557.
30. Bieberich E., Silva J., Wang G., et al. Selective apoptosis of pluripotent mouse and human stem cells by novel ceramide analogues prevents teratoma formation and enriches for neural precursors in ES cell-derived neural transplants. J. Cell Biol. 2004, 167:723-734.
31. Sánchez-Pernaute R., Studer L., Ferrari D., et al. Long-term survival of dopamine neurons derived from parthenogenetic primate embryonic stem cells (cyno-1) after transplantation. Stem Cells 2005, 23:914-922.
32. Brederlau A., Correia A.S., Anisimov S.V., et al. Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson's disease: effect of in vitro differentiation on graft survival and teratoma formation. Stem Cells 2006, 24:1433-1440.
33. Trounson A. Human embryonic stem cells: mother of all cell and tissue types. Reprod. Biomed. Online 2002, 4:58-63.