The Discovery of Stromatolites Developing at 3570 m above Sea Level in a High-Altitude Volcanic Lake Socompa, Argentinean Andes

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Farías, María E ^a   Rascovan, Nicolás ^b   Toneatti, Diego M ^a   Albarracín, Virginia H ^a,c,d   Flores, María R ^a   Poiré, Daniel G ^e   Collavino, Mónica M ^e   Aguilar, O Mario ^e   Vazquez, Martin P ^b   Polerecky, Lubos ^f  

^a PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLÓGICOS PROIMI CONICET   (Argentina)

^b Instituto de Agrobiotecnología de Rosario INDEAR Predio CCT Rosario   (Argentina)

^c UNIVERSIDAD NACIONAL DE TUCUMÁN   (Argentina)

^d MAX PLANCK INSTITUTE FOR CHEMICAL ENERGY CONVERSION   (Germany)

^e UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^f MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALTITUDE; ARGENTINA; ARTICLE; DEINOCOCCUS; DESULFOBACTERALES; DIATOM; EVOLUTIONARY ADAPTATION; GEOGRAPHIC AND GEOLOGICAL PHENOMENA; MICROBIAL COMMUNITY; PHYSICAL CHEMISTRY; POPULATION ABUNDANCE; RHODOBACTERACEAE; SEA LEVEL; SPECIES DIVERSITY; SPECIES DOMINANCE; SPIROCHAETA; STROMATOLITE; THERMUS; ULTRAVIOLET RADIATION;

ADAPTATION, PHYSIOLOGICAL; ALTITUDE; ARSENIC; BASE SEQUENCE; BIOLOGICAL EVOLUTION; CYANOBACTERIA; DIATOMS; DNA, BACTERIAL; ECOSYSTEM; GEOLOGIC SEDIMENTS; LAKES; MOLECULAR SEQUENCE DATA; PHYLOGENY; RHODOBACTERACEAE; SALINITY; SPIROCHAETA; TEMPERATURE; ULTRAVIOLET RAYS;

AMPHORA; BACILLARIOPHYTA; CYANOBACTERIA; DEINOCOCCUS-THERMUS; DESULFOBACTERALES; MICROCOLEUS SP.; RHODOBACTERACEAE; SPIROCHAETES;

EID: 84872126675     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0053497     Document Type: Article

References (107)

1. Walter MR (1976) Stromatolites. Amsterdam: Elsevier.
2. Burne RV, Moore LS, (1987) Microbialites; organosedimentary deposits of benthic microbial communities. Palaios 2: 241-254.
3. Allwood A, Walter M, Kamber B, Marshall CP, Burch IW, (2006) Stromatolite reef from the Early Archaean era of Australia. Nature 441: 714-718.
4. Schopf J, (2006) Fossil evidence of Archaean life. Philosophical Transactions of the Royal Society B 361: 869-885.
5. Kasting JK, (1991) Box models for the evolution of atmospheric oxygen: an update. Palaeogeography, Palaeoclimatology, Palaeoecology 97: 125-131.
6. Holland HD (1994) Early Proterozoic atmospheric change. In: Bengston S, editor. Early Life on Earth. Columbia University Press. pp. 237-244.
7. Garrett P, (1970) Phanerozoic stromatolites: noncompetitive ecologic restriction by grazing and burrowing animals. Science 169: 171-173.
8. Awramik SM, (1971) Precambrian Columnar Stromatolite Diversity: Reflection of Metazoan Appearance. Science 174: 825-826.
9. Walter MR, Heys GR, (1985) Links between the rise of the metazoans and the decline of stromatolites. Precambrian Research 29: 149-174.
10. Hoffman P (1976) Stromatolite morphogenesis in Shark Bay, Western Australia. In: Walter MR, editor. Stromatolites. Amsterdam: Elsevier Scientific Publishing Company. pp. 261-272.
11. Playford PE, Cockbain AE (1976) Modern algal stromatolites at Hamelin Pool, a hypersaline barred basin in Shark Bay, Western Australia. In: Walter MR, editor. Stromatolites. Amsterdam: Elsevier Scientific Publishing Company. p. 101.
12. Walter MR, Bauld J, Brock TD, (1972) Siliceous algal and bacterial stromatolites in hot spring and geyser effluents of yellowstone national park. Science 178: 402-405.
13. Chizuru T, Akihiro K, (2005) Depositional processes of travertine developed at Shionoha hot spring, Nara Prefecture, Japan. Journal of the Geological Society of Japan 111: 751-764.
14. Takashima C, Kano A, (2008) Microbial processes forming daily lamination in a stromatolitic travertine. Sedimentary Geology 208: 114-119.
15. Jones B, Renaut RW, Konhauser KO, (2005) Genesis of large siliceous stromatolites at Frying Pan Lake, Waimangu geothermal field, North Island, New Zealand. Sedimentology 52: 1229-1252.
16. Dravis JJ, (1983) Hardened subtidal stromatolites. Science 219: 385-386.
17. Reid RP, Macintyre IG, Brown KM, Steneck RS, Miller T, (1995) Modern marine stromatolites in the Exuma Cays, Bahamas-uncommonly common. Facies 33: 1-17.
18. Souza V, Espinosa-Asuar L, Escalante AE, Eguiarte LE, Farmer J, et al. (2006) An endangered oasis of aquatic microbial biodiversity in the Chihuahuan desert. Proceedings of the National Academy of Sciences of the United States of America 103: 6565-6570.
19. Souza V, Siefert JL, Escalante AE, Elser JJ, Eguiarte LE, (2012) The Cuatro Ciénegas Basin in Coahuila, Mexico: an astrobiological precambrian park. Astrobiology 12: 641-647.
20. Pedley M (2000) Ambient temperature freshwater microbial tufas. In: Riding R, Awramik SM, editors. Microbial Sediments. Heidelberg: Springer. pp. 179-186.
21. Farías ME, Poiré DG, Arrouy MJ, Albarracín VH, (2011) Modern stromatolite ecosystems at alkaline and hypersaline high-altitude lakes in the Argentinean Puna. In: Tewari VC, Seckbach J, editors. Stromatolites: interaction of microbes with sediments. Springer Science+Business Media B. V: pp. 427-440.
22. Zappettini EO, Blasco G, (2001) Hoja Geológica 2569-II, Socompa, provincia de Salta, República Argentina. Servicio Geológico Minero Argentino Buletin 26: 65.
23. Cabrera S, Bozzo S, Fuenzalida H, (1995) Variations in UV radiation in Chile. Journal of Photochemistry and Photobiology B: Biology 28: 137-142.
24. Dupraz C, Visscher PT, (2005) Microbial lithification in marine stromatolites and hypersaline mats. Trends in microbiology 13: 429-438.
25. Dupraz C, Reid RP, Braissant O, Decho AW, Norman RS, et al. (2009) Processes of carbonate precipitation in modern microbial mats. Earth-Science Reviews 96: 141-162.
26. Gallagher K, Dupraz C, Braissant O, Norman R, Decho A, et al. (2010) Mineralization of sedimentary biofilms: modern mechanistic insights. In: Columbus F, editor. Biofilm: Formation, Development and Properties. Nova Science Publishers.
27. Decho A, (2010) Overview of biopolymer-induced mineralization: What goes on in biofilms? Ecological Engineering 36: 137-144.
28. Visscher PT, Stolz JF, (2005) Microbial mats as bioreactors: populations, processes, and products. Palaeogeography, Palaeoclimatology, Palaeoecology 219: 87-100.
29. Lewis J, Harris ASD, Jones KJ, Edmonds RL, (1999) Long-term survival of marine planktonic diatoms and dinoflagellates in stored sediment samples. Journal of Plankton Research 21: 343-354.
30. McQuoid M, Godhe A, Nordberg K, (2002) Viability of phytoplankton resting stages in the sediments of a coastal Swedish fjord. European Journal of Phycology 37: 191-201.
31. Ribeiro S, Berge T, Lundholm N, Andersen TJ, Abrantes F, et al. (2011) Phytoplankton growth after a century of dormancy illuminates past resilience to catastrophic darkness. Nature communications 2: 311.
32. Kamp A, de Beer D, Nitsch JL, Lavik G, Stief P, (2011) Diatoms respire nitrate to survive dark and anoxic conditions. Proceedings of the National Academy of Sciences of the United States of America 108: 5649-5654.
33. Houston J, Hartley AJ, (2003) The central Andean west-slope rainshadow and its potential contribution to the origin of hyper-aridity in the Atacama desert. International Journal of Climatology 23: 1453-1464.
34. Piacentini RD, Cede A, Bárcena H, (2003) Extreme solar total and UV irradiances due to cloud effect measured near the summer solstice at the high-altitude desertic plateau Puna of Atacama (Argentina). Journal of Atmospheric and Solar-Terrestrial Physics 65: 727-731.
35. Luccini E, Cede A, Piacentini R, Villanueva C, Canziani P, (2006) Ultraviolet climatology over Argentina. Journal of Geophysical Research 111: D17312.
36. McKenzie RL, Bjorn LO, Bais A, Ilyasd M, (2003) Changes in biologically active ultraviolet radiation reaching the Earth's surface. Photochemical & Photobiological Sciences 2: 5-15.
37. Schmidt SK, Nemergut DR, Miller AE, Freeman KR, King AJ, et al. (2009) Microbial activity and diversity during extreme freeze-thaw cycles in periglacial soils, 5400 m elevation, Cordillera Vilcanota, Peru. Extremophiles 13: 807-816.
38. Lynch R, King A, Farías M, Sowell C, Vitry C, et al. (2012) The potential for microbial life in the highest-elevation (>6000 masl) mineral soils of the Atacama region. Journal of Geophysical Research-Biogeosciences 117: G02028.
39. Halloy S, (1991) Islands of life at 6000 m altitude: the environment of the highest autotrophic communities on Earth (Socompa Volcano, Andes). Arctic and Alpine Research 23: 247-262.
40. Costello EK, Halloy SRP, Reed SC, Sowell P, Schmidt SK, (2009) Fumarole-Supported Islands of Biodiversity within a Hyperarid, High-Elevation Landscape on Socompa Volcano, Puna de Atacama, Andes. Applied and Environmental Microbiology 75: 735-747.
41. Berelson WM, Corsetti FA, Pepe-Ranney C, Hammond DE, Beaumont W, et al. (2011) Hot spring siliceous stromatolites from Yellowstone National Park: assessing growth rate and laminae formation. Geobiology 9: 411-424.
42. Foster JS, Green SJ, Ahrendt SR, Golubic S, Reid RP, et al. (2009) Molecular and morphological characterization of cyanobacterial diversity in the stromatolites of Highborne Cay, Bahamas. The ISME journal 3: 573-587.
43. Santos F, Pena A, Nogales B, Soria E, Garcia del Cura MA, et al. (2010) Bacterial diversity in modern freshwater stromatolits from Ruidera Pools Natural Park, Spain. Systematic and Applied Microbiology 33: 209-221.
44. Foster JS, Green SJ, (2011) Microbial diversity in modern stromatolites. In: Tewari VC, Seckbach J, editors. Stromatolites: interaction of microbes with sediments. Heidelberg: Springer Science+Business Media B. V: pp. 383-405.
45. Pepe-Ranney C, Berelson WM, Corsetti F a, Treants M, Spear JR, (2012) Cyanobacterial construction of hot spring siliceous stromatolites in Yellowstone National Park. Environmental Microbiology 14: 1182-1197.
46. Bebout B, Garcia-Pichel F, (1995) UV B-Induced Vertical Migrations of Cyanobacteria in a Microbial Mat. Applied and Environmental Microbiology 61: 4215-4222.
47. Nadeau T-L, Howard-Williams C, Castenholz, Richard W, (1999) Effects of solar UV and visible irradiance on photo- synthesis and vertical migration of Oscillatoria sp. (Cyanobacteria) in an Antarctic microbial mat. Aquatic Microbial Ecology 20: 231-243.
48. Choi J, Chung Y, Moon Y, Kim C, Watanabe M, et al. (1999) Photomovement of the gliding cyanobacterium Synechocystis sp. PCC 6803. Photochemistry and Photobiology 70: 95-102.
49. Ng W, Grossman AR, Bhaya D, (2003) Multiple light inputs control phototaxis in Synechocystis sp. strain PCC6803. Journal of Bacteriology 185: 1599-1607.
50. Krause-Jensen D, Sand-Jensen K, (1998) Light attenuation and photosynthesis of aquatic plant communities. Limnology and Oceanography 43: 396-407.
51. Gray MW, Burger G, Lang BF, (1999) Mitochondrial evolution. Science 283: 1476-1481.
52. Emelyanov V, (2003) Mitochondrial connection to the origin of the eukaryotic cell. European Journal of Biochemistry 270: 1599-1618.
53. Futuyama DJ, (2005) On Darwin's Shoulders. Natural History 114: 64-68.
54. Papineau D, Walker JJ, Mojzsis SJ, Pace NR, (2005) Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia. Applied and Environmental Microbiology 71: 4822-4832.
55. Goh F, Allen MA, Leuko S, Kawaguchi T, Decho AW, et al. (2009) Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay. The ISME journal 3: 383-396.
56. Baumgartner LK, Spear JR, Buckley DH, Pace NR, Reid RP, et al. (2009) Microbial diversity in modern marine stromatolites, Highborne Cay, Bahamas. Environmental Microbiology 11: 2710-2719.
57. Breitbart M, Hoare A, Nitti A, Siefert J, Haynes M, et al. (2009) Metagenomic and stable isotopic analyses of modern freshwater microbialites in Cuatro Ciénegas, Mexico. Environmental Microbiology 11: 16-34.
58. Lemee L, Peuchant E, Clerc M, Brunner M, Pfander H-P, (1997) Deinoxanthin: a new carotenoid isolated from Deinococcus radiodurans. Tetrahedron 53: 919-926.
59. Tian B, Hua Y, (2010) Carotenoid biosynthesis in extremophilic Deinococcus-Thermus bacteria. Trends in microbiology 18: 512-520.
60. Garrity GM, Brenner DJ, Krieg NR, Staley JT (2005) Bergey's Manual of Systematic Bacteriology. New York: Springer. p.
61. Berlanga M, Aas JA, Paster BJ, Boumenna T, Dewhirst FE, et al. (2008) Phylogenetic diversity and temporal variation in the Spirochaeta populations from two Mediterranean microbial mats. International Journal of Microbiology 4: 267-274.
62. Ley RE, Harris JK, Wilcox J, Spear JR, Miller SR, et al. (2006) Unexpected Diversity and Complexity of the Guerrero Negro Hypersaline Microbial Mat Unexpected Diversity and Complexity of the Guerrero Negro Hypersaline Microbial Mat. Applied and Environmental Microbiology 72: 3685-3695.
63. Raymond J, Siefert JL, Staples CR, Blankenship RE, (2004) The natural history of nitrogen fixation. Molecular Biology and Evolution 21: 541-554.
64. Steppe TF, Paerl HW, (2002) Potential N-2 fixation by sulfate reducing bacteria in a marine intertidal microbial mat. Aquatic Microbial Ecology 28: 1-12.
65. Omoregie EO, Crumbliss LL, Bebout BM, Zehr JP, (2004) Comparison of diazotroph community structure in Lyngbya sp. and Microcoleus chthonoplastes dominated microbial mats from Guerrero Negro, Baja, Mexico. FEMS Microbial Ecology 47: 305-318.
66. Moisander PH, Shiue L, Steward GF, Jenkins BD, Bebout BM, et al. (2006) Application of a nifH oligonucleotide microarray for profiling diversity on N2-fixing microorganisms in marine microbial mats. Environmental Microbiology 8: 1721-1735.
67. Falcon LI, Cerritos R, Eguiarte LE, Souza V, (2007) Nitrogen fixation in microbial mat and stromatolite communities from Cuatro Cienegas, Mexico. Microbial Ecology 54: 363-373.
68. Kowalchuk GA, Stephen JR, (2001) Ammonia-oxidizing bacteria: a model for molecular microbial ecology. Annual Reviews in Microbiology 55: 485-529.
69. Francis CA, Roberts KJ, Bernan JM, Santoro AE, Oakley BB, (2005) Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proceedings of the National Academy of Sciences of the United States of America 102: 14683-14688.
70. Hu A, Yao T, Jiao N, Liu Y, Yang Z, et al. (2010) Community structures of ammonia-oxidising archaea and bacteria in high-altitude lakes on the Tibetan Plateau. Freshwater Biology 55: 2375-2390.
71. Lozupone CA, Knight R, (2007) Global patterns in bacterial diversity. Proceedings of the National Academy of Sciences of the United States of America 104: 11436-11440.
72. Demergasso C, Casamayor EO, Chong G, Galleguillos P, Escudero L, et al. (2004) Distribution of prokaryotic genetic diversity in athalassohaline lakes of the Atacama Desert, Northern Chile. FEMS Microbiology Ecology 48: 57-69.
73. Ordoñez O, Flores M, Dib J, Paz A, Farias ME, (2009) Extremophile culture collection from Andean lakes: extreme pristine environments that host a wide diversity of microorganisms with tolerance to UV radiation. Microbial Ecology 58: 461-473.
74. Dorador C, Meneses D, Urtivia V, Demergasso C, Vila I, et al. (2009) Diversity of Bacteroidetes in high altitude saline evaporitic basins in northern Chile. Journal of Geophysical Research-Biogeosciences 114: G00D05.
75. Dorador C, Vila I, Remonsellez F, Imhoff J, Witzel K, (2010) Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano. FEMS Microbiology Ecology. 73: 291-302.
76. Gramain A, Díaz G, Demergasso C, Lowenstein TK, McGenity TJ, (2011) Archaeal diversity along a subterranean salt core from the Salar Grande (Chile). Environmental Microbiology 13: 2105-2121.
77. Lassen C, Ploug H, Jørgensen BB, (1992) Microalgal photosynthesis and spectral scalar irradiance in coastal marine sediments of Limnfjorden, Denmark. Limnol Oceanogr 37: 760-772.
78. Revsbech NP, Jørgensen BB, (1986) Microelectrodes: their use in microbial ecology. Adv Microb Ecol 9: 293-352.
79. Kühl M, Steuchart C, Eickert G, Jeroschewski P, (1998) A H2S microsensor for profiling biofilms and sediments: Application in an acidic lake sediment. Aquat Microb Ecol 15: 201-209.
80. de Beer D, Glud A, Epping E, Kühl M, (1997) A fast responding CO2 micro-electrode for profiling sediments, microbial mats and biofilms. Limnol Oceanogr 42: 1590-1600.
81. Weber M, Faerber P, Meyer V, Lott C, Eickert G, et al. (2007) In situ applications of a new diver-operated motorized microsensor profiler. Env Sci Technol 41: 6210-6215.
82. Polerecky L, Bachar A, Schoon R, Grinstein M, Jørgensen BB, et al. (2007) Contribution of Chloroflexus respiration to oxygen cycling in a hypersaline microbial mat from Lake Chiprana, Spain. Environmental microbiology 9: 2007-2024.
83. Al-Najjar MAA, de Beer D, Jørgensen BB, Kühl M, Polerecky L, (2010) Conversion and conservation of light energy in a photosynthetic microbial mat ecosystem. The ISME Journal 4: 440-449.
84. Garcia HE, Gordon LI, (1992) Oxygen solubility in seawater: Better fitting equations. Limnology and Oceanography 37: 1307-1312.
85. Li Y-H, Gregory S, (1974) Diffusion of ions in sea water and deep-sea sediments. Geochim Cosmochim Acta 38: 703-714.
86. Polerecky L, Bissett A, Al-Najjar M, Faerber P, Osmers H, et al. (2009) Modular spectral imaging system for discrimination of pigments in cells and microbial communities. Applied and Environmental Microbiology 75: 758.
87. Sode K, Horikoshi K, Takeyama H, Nakamura N, Matsunaga T, (1991) Online monitoring of marine cyanobacterial cultivation based on phycocyanin fluorescence. Journal of Biotechnology 21: 209-217.
88. Borrego CM, Garcia-Gil LJ, (1994) Separation of bacteriochlorophyll homologs from green photosynthetic sulphur bacteria by reversed-phase HPLC. Photosynthesis Research 41: 157-163.
89. Wright S, Jeffrey S, Mantoura RFC, Llewelyn CA, Bjornland T, et al. (1991) Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology-Progress Series 77: 183-196.
90. Porra R, Thompson W, Kriedemann P, (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta 975: 384-394.
91. Permentier HP, Neerken S, Schmidt KA, Overmann J, Amesz J, (2000) Energy transfer and charge separation in the purple non-sulfur bacterium Roseospirillum parvum. Biochimica et Biophysica Acta 1460: 338-345.
92. Stanier RY, Smith JHC, (1960) The chlorophylls of green bacteria. Biochimica et Biophysica Acta 41: 478-484.
93. Chennu A, Volkenborn N, Polerecky L (2011) Mapping the in situ distribution of microphytobenthos in coastal sediments Talk at the International symposium "Trophic Significance of microbial biofilms in tidal flats", La Rochelle.
94. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, et al. (2010) QIIME allows analysis of high-throughput community sequencing data. Nature methods 7: 335-336.
95. Zehr JP, McReynolds LA, (1989) The use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii. Applied and Environmental Microbiology 55: 2522-2526.
96. Burns JA, Zehr JP, Capone DG, (2002) Nitrogen-fixing phylotypes of Chesapeake Bay and Neuse River Estuary sediments. Microbial Ecology 44: 336-343.
97. Junier P, Kim OS, Molina V, Limburg P, Junier T, et al. (2008) Comparative in silico analysis of PCR primers suited for diagnostics and cloning of ammonia monooxygenase genes from ammonia-oxidizing bacteria. FEMS Microbiology Ecology 64: 141-152.
98. Rotthauwe JH, Witzel KP, Liesack W, (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology 63: 4704-4712.
99. Tamura K, Dudley J, Nei M, Kumar S, (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24: 1596-1599.
100. Schloss PD, Handelsman J, (2005) Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Applied and Environmental Microbiology 71: 1501-1506.
101. Konstantinidis KT, Ramette A, Tiedje JM, (2006) The bacterial species definition in the genomic era. Philosophical Transactions of the Royal Society B 361: 1929-1940.
102. Qian P, Wang Y, Lee O, Lau S, Yang J, et al. (2011) Vertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencing. The ISME 5: 507-518.
103. Lanzén A, Jørgensen SL, Bengtsson MM, Jonassen I, Øvreås L, et al. (2011) Exploring the composition and diversity of microbial communities at the Jan Mayen hydrothermal vent field using RNA and DNA. FEMS Microbiology Ecology 77: 577-589.
104. Ionescu D, Siebert C, Polerecky L, Munwes YY, Lott C, et al. (2012) Microbial and Chemical Characterization of Underwater Fresh Water Springs in the Dead Sea. PLoS ONE 7: e38319.
105. Sahl J, Fairfield N, Harris J, Wettergreen D, Stone W, et al. (2010) Novel microbial diversity retrieved by autonomous robotic exploration of the world's deepest vertical phreatic sinkhole. Astrobiology 10: 201-213.
106. Neilson J, Quade J, Ortiz M, Nelson W, Legatzki A, et al. (2012) Life at the hyperarid margin: novel bacterial diversity in arid soils of the Atacama Desert, Chile. Extremophiles 16: 553-566.
107. Mobberley J, Ortega M, Foster J, (2012) Comparative microbial diversity analyses of modern marine thrombolitic mats by barcoded pyrosequencing. Environmental Microbiology 14: 82-100.