Design and evaluation of a microfluidic system for inhibition studies of yeast cell signaling

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 8458, Issue , 2012, Pages

  Hamngren, Charlotte ^a,b   Dinér, Peter ^a,c   Grøtli, Morten ^a   Goksör, Mattias ^a   Adiels, Caroline B ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Microfluidics; Optical manipulation; Signal transduction pathways; Single cell analysis

Indexed keywords

BIOLOGICAL TECHNIQUES; CELL ARRAY; CELL SIGNALING; CELL-TO-CELL VARIATION; DIFFUSION RATE; DYNAMIC SIGNALS; HOG PATHWAY; INLET CHANNELS; KINASE INHIBITORS; MICRO FLUIDIC SYSTEM; NUCLEAR LOCALIZATION; OPTICAL MANIPULATION; OSMOLARITY; OSMOTIC STRESS; SEQUENTIAL TREATMENTS; SIGNAL TRANSDUCTION PATHWAYS; SINGLE-CELL LEVEL; SINGLECELL ANALYSIS; SUBCELLULAR LOCALIZATIONS; YEAST CELL;

CELLS; CYTOLOGY; FLUIDIC DEVICES; GLYCEROL; MICROFLUIDICS; MICROMANIPULATORS; OPTICAL TWEEZERS; SIGNAL TRANSDUCTION; YEAST;

MOLECULAR BIOLOGY;

EID: 84872069646     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.929728     Document Type: Conference Paper

References (16)

1. J. C. Mell, and S. M. Burgess, [Yeast as a Model Genetic Organism] John Wiley & Sons, Ltd, (2001).
2. D. Botstein, S. A. Chervitz, and J. M. Cherry, "Genetics - Yeast as a model organism," Science, 277(5330), 1259-1260 (1997).
3. W. H. Mager, and J. Winderickx, "Yeast as a model for medical and medicinal research," Trends in Pharmacological Sciences, 26(5), 265-273 (2005).
4. L. H. Hartwell, "Yeast and cancer," Bioscience Reports, 24(4-5), 523-544 (2004).
5. E. De Nadal, F. X. Real, and F. Posas, "Mucins, osmosensors in eukaryotic cells?," Trends in Cell Biology, 17(12), 571-574 (2007).
6. E. Klipp, B. Nordlander, R. Kruger et al., "Integrative model of the response of yeast to osmotic shock," Nat Biotechnol, 23(8), 975-82 (2005).
7. D. Di Carlo, and L. P. Lee, "Dynamic single-cell analysis for quantitative biology," Analytical Chemistry, 78(23), 7918-7925 (2006).
8. H. A. Svahn, and A. van den Berg, "Single cells or large populations?," Lab on a Chip, 7(5), 544-546 (2007).
9. A. Ashkin, J. M. Dziedzic, and T. Yamane, "Optical trapping and manipulation of single cells using infrared laser beams," Nature, 330(6150), 769-71 (1987).
10. E. Eriksson, K. Sott, F. Lundqvist et al., "A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning," Lab on a Chip, 10(5), 617-625 (2010).
11. P. Dinér, J. Veide Vilg, J. Kjellén et al., "Design, Synthesis, and Characterization of a Highly Effective Hog1 Inhibitor: A Powerful Tool for Analyzing MAP Kinase Signaling in Yeast," PLoS ONE, 6(5), e20012 (2011).
12. K. Sott, E. Eriksson, and M. Goksör, [Biomolecular Separation and Analysis] Caister Academic Press, Norwich(2009).
13. D. C. Duffy, J. C. McDonald, O. J. A. Schueller et al., "Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)," Analytical Chemistry, 70(23), 4974-4984 (1998).
14. D. Mislovicova, J. Masarova, J. Svitel et al., "Influence of mannan epitopes in glycoproteins - Concanavalin A interaction. Comparison of natural and synthetic glycosylated proteins," International Journal of Biological Macromolecules, 30(5), 251-258 (2002).
15. A.-K. Gustavsson, B. C. Adiels, and M. Goksör, "Induction of sustained glycolytic oscillations in single yeast cells using microfluidics and optical tweezers," Proc. SPIE 8458-34, (2012).
16. M. Smedh, C. Beck, K. Sott et al., "CellStress - open source image analysis program for single-cell analysis," Proc. SPIE, 7762,, 77622N-77622N-11 (2010).