Cross validation of liquid chromatography-mass spectrometry and lectin array for monitoring glycosylation in fed-batch glycoprotein production

Molecular Biotechnology

Volumn 51, Issue 3, 2012, Pages 272-282

  Hayes, Catherine A ^a,b   Doohan, Roisin ^a   Kirkley, David ^c   Leister, Kirk ^c   Harhen, Brendan ^a   Savage, Angela V ^a   Karlsson, Niclas G ^a,b  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

^c BRISTOL MYERS SQUIBB COMPANY   (United States)

Author keywords

Acetylation; Glycosylation; High mannose; Lectin array; Mass spectrometry; N linked oligosaccharides

Indexed keywords

BIOPHARMACEUTICAL INDUSTRY; HIGH MANNOSE; LECTIN ARRAY; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; N-ACETYLNEURAMINIC ACID; N-GLYCOLYLNEURAMINIC ACID; N-LINKED; RECOMBINANT GLYCOPROTEINS;

ACETYLATION; ESTERIFICATION; GLYCOPROTEINS; MASS SPECTROMETRY; OLIGOSACCHARIDES; PROTEINS;

GLYCOSYLATION;

EPITOPE; LECTIN; N ACETYLNEURAMINIC ACID; N GLYCOLOYLNEURAMINIC ACID; SIALIC ACID; GALACTOSE; GLYCOPROTEIN; MANNOSE; OLIGOSACCHARIDE;

ANTENNA; ARTICLE; FED BATCH CULTURE; FUCOSYLATION; LIQUID CHROMATOGRAPHY; MASS FRAGMENTOGRAPHY; PROCESS MONITORING; PROTEIN ISOLATION; PROTEIN MODIFICATION; SIALYLATION; ANIMAL; BIOREACTOR; BIOSYNTHESIS; CHEMISTRY; CHO CELL; CRICETULUS; GLYCOSYLATION; HAMSTER; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MICROARRAY ANALYSIS; REGRESSION ANALYSIS; REPRODUCIBILITY;

ANIMALS; BIOREACTORS; CHO CELLS; CHROMATOGRAPHY, LIQUID; CRICETINAE; CRICETULUS; GALACTOSE; GLYCOPROTEINS; GLYCOSYLATION; LECTINS; MANNOSE; MASS SPECTROMETRY; MICROARRAY ANALYSIS; N-ACETYLNEURAMINIC ACID; OLIGOSACCHARIDES; REGRESSION ANALYSIS; REPRODUCIBILITY OF RESULTS;

EID: 84871864393     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9465-8     Document Type: Article

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