Tear lacritin levels by age, sex, and time of day in healthy adults

Investigative Ophthalmology and Visual Science

Volumn 53, Issue 10, 2012, Pages 6610-6616

  Seifert, Kyle ^a   Gandia, Natasha C ^a   Wilburn, Jennifer K ^a   Bower, Kraig S ^b   Sia, Rose K ^c   Ryan, Denise S ^c   Deaton, Michael L ^a   Still, Katherine M ^a   Vassilev, Veronica C ^a   Laurie, Gordon W ^d   McKown, Robert L ^a  

^a JAMES MADISON UNIVERSITY   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c WALTER REED ARMY MEDICAL CENTER   (United States)

^d UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LACRITIN; LYSOZYME; RECOMBINANT PROTEIN; TEAR PROTEIN; UNCLASSIFIED DRUG; EYE PROTEIN; GLYCOPROTEIN; LACRT PROTEIN, HUMAN; PEPTIDE FRAGMENT; TEAR PROTEINS;

ADULT; AFFINITY CHROMATOGRAPHY; AGE; ARTICLE; BLEPHARITIS; CHEMILUMINESCENCE IMMUNOASSAY; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENDER; HUMAN; HUMAN EXPERIMENT; LACRIMAL FLUID; MALE; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; WESTERN BLOTTING; ADOLESCENT; AGING; ANIMAL; CHEMISTRY; IMMUNOLOGY; METABOLISM; MIDDLE AGED; PHYSIOLOGY; RABBIT; SEX RATIO; TIME;

ADOLESCENT; ADULT; AGING; ANIMALS; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; EYE PROTEINS; FEMALE; GLYCOPROTEINS; HUMANS; MALE; MIDDLE AGED; PEPTIDE FRAGMENTS; RABBITS; RECOMBINANT PROTEINS; SEX DISTRIBUTION; TEARS; TIME FACTORS; YOUNG ADULT;

EID: 84871861408     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.11-8729     Document Type: Article

References (40)

1. Sanghi S, Kumar R, Lumsden A, et al. cDNA and genomic cloning of lacritin: a novel secretion enhancing factor from the human lacrimal gland. J Mol Biol. 2001;310:127-139.
2. Samudre SS, Lattanzio FA, Lossen V, et al. Lacritin, a novel human tear glycoprotein, promotes sustained basal tearing and is well tolerated. Invest Ophthalmol Vis Sci. 2010;52:6265-6270.
3. Tomlinson A, Lemp MA. Dry eye: have we found the perfect diagnostic? Ophthalmol Times Eur. 2008;4. Available at: http://www.oteurope.com/ophthalmologytimeseurope/Dryeye-have-we-found-the-perfect-diagnostic/ArticleStandard/Article/ detail/520993. Accessed April 22, 2012.
4. Korb DR. Survey of preferred tests for diagnosis of the tear film and dry eye. Cornea. 2000;19:483-486.
5. Lemp MA, Baudouin C, Baum J, et al. The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye Workshop. Ocul Surf. 2007;5:75-92.
6. Sullivan BD, Crews LA, Sonmez B, et al. Clinical utility of objective tests for dry eye disease: variability over time and implications for clinical trials and disease management. Cornea. 2012;31:1000-1008.
7. Szalai E, Berta A, Szekanecz Z, Szucs G, Modis L Jr. Evaluation of tear osmolarity in non-Sjögren and Sjögren syndrome dry eye patients with the TearLab system. Cornea. 2012;31:867-871.
8. Zhou L, Zhao SZ, Koh SK, et al. In-depth analysis of the human tear proteome. J Proteomics. 2012;75:3877-3885.
9. McKown RL, Wang N, Raab RW, et al. Lacritin and other new proteins of the lacrimal functional unit. Exp Eye Res. 2009;88: 848-858.
10. Nichols JJ, Green-Church KB. Mass spectrometry-based proteomic analyses in contact lens-related dry eye. Cornea. 2009;28:1109-1117.
11. Koo BS, Lee DY, Ha HS, Kim JC, Kim CW. Comparative analysis of the tear protein expression in blepharitis patients using twodimensional electrophoresis. J Proteome Res. 2005;4:719-724.
12. Janssen PT, van Bijsterveld OP. The relations between tear fluid concentrations of lysozyme, tear-specific prealbumin and lactoferrin. Exp Eye Res. 1983;36:773-779.
13. Ng V, Cho P, Mak S, Lee A. Variability of tear protein levels in normal young adults: between-day variation. Graefes Arch Clin Exp Ophthalmol. 2000;238:892-899.
14. Ng V, Cho P, Wong F, Chan Y. Variability of tear protein levels in normal young adults: diurnal (daytime) variation. Graefes Arch Clin Exp Ophthalmol. 2001;239:257-263.
15. Gachon AM, Richard J, Dastugue B. Human tears: normal protein pattern and individual protein determinations in adults. Curr Eye Res. 1982;2:301-308.
16. Ananthi S, Santhosh RS, Nila MV, Prajna NV, Lalitha P, Dharmalingam K. Comparative proteomics of human male and female tears by two-dimensional electrophoresis. Exp Eye Res. 2011;92:454-463.
17. Tomosugi N, Kitagawa K, Takahashi N, Sugai S, Ishikawa I. Diagnostic potential of tear proteomic patterns in Sjögren's syndrome. J Proteome Res. 2005;4:820-825.
18. Grus FH, Podust VN, Bruns K, et al. SELDI-TOF-MS ProteinChip array profiling of tears from patients with dry eye. Invest Ophthalmol Vis Sci. 2005;46:863-876.
19. Zhou L, Beuerman RW, Foo Y, Liu S, Ang LP, Tan DT. Characterisation of human tear proteins using high-resolution mass spectrometry. Ann Acad Med Singapore. 2006;35:400-407.
20. Li N, Wang N, Zheng J, et al. Characterization of human tear proteome using multiple proteomic analysis techniques. J Proteome Res. 2005;4:2052-2061.
21. de Souza GA, Godoy LM, Mann M. Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors. Genome Biol. 2006;7:R72.
22. McGill JI, Liakos GM, Goulding N, Seal DV. Normal tear protein profiles and age-related changes. Br J Ophthalmol. 1984;68: 316-320.
23. Fullard RJ, Snyder C. Protein levels in nonstimulated and stimulated tears of normal human subjects. Invest Ophthalmol Vis Sci. 1990;31:1119-1126.
24. Fullard RJ, Tucker DL. Changes in human tear protein levels with progressively increasing stimulus. Invest Ophthalmol Vis Sci. 1991;32:2290-2301.
25. Sack R, Conradi L, Beaton A, Sathe S, McNamara N, Leonardi A. Antibody array characterization of inflammatory mediators in allergic and normal tears in the open and closed eye environments. Exp Eye Res. 2007;85:528-538.
26. Sack RA, Conradi L, Krumholz D, Beaton A, Sathe S, Morris C. Membrane array characterization of 80 chemokines, cytokines, and growth factors in open-and closed-eye tears: angiogenin and other defense system constituents. Invest Ophthalmol Vis Sci. 2005;46:1228-1238.
27. Karns K, Herr AE. Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay. Anal Chem. 2011;83:8115-8122.
28. Wang J, Wang N, Xie J, et al. Restricted epithelial proliferation by lacritin via PKCalpha-dependent NFAT and mTOR pathways. J Cell Biol. 2006;174:689-700.
29. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972;109:129-135.
30. Jones DT, Monroy D, Pflugfelder SC. A novel method of tear collection: comparison of glass capillary micropipettes with porous polyester rods. Cornea. 1997;16:450-458.
31. Lee HK, Lee KS, Kim HC, Lee SH, Kim EK. Nerve growth factor concentration and implications in photorefractive keratectomy vs laser in situ keratomileusis. Am J Ophthalmol. 2005; 139:965-971.
32. Lee HK, Ryu IH, Seo KY, Hong S, Kim HC, Kim EK. Topical 0.1% prednisolone lowers nerve growth factor expression in keratoconjunctivitis sicca patients. Ophthalmology. 2006;113: 198-205.
33. Ozyildirim AM, Wistow GJ, Gao J, et al. The lacrimal gland transcriptome is an unusually rich source of rare and poorly characterized gene transcripts. Invest Ophthalmol Vis Sci. 2005;46:1572-1580.
34. Plikaytis BD, Turner SH, Gheesling LL, Carlone GM. Comparisons of standard curve-fitting methods to quantitate neisseria meningitidis group A polysaccharide antibody levels by enzyme-linked immunosorbent assay. J ClinMicrobiol. 1991;29:1439-1446.
35. Sorensen K, Brodbeck U. Assessment of coating-efficiency in ELISA plates by direct protein determination. J Immunol Methods. 1986;95:291-293.
36. Sen DK, Sarin GS. Biological variations of lysozyme concentration in the tear fluids of healthy persons. Br J Ophthalmol. 1986;70:246-248.
37. Farris RL. Tear analysis in contact lens wearers. Trans Am Ophthalmol Soc. 1985;83:501-545.
38. Marcozzi G, Liberati V, Madia F, Centofanti M, de Feo G. Ageand gender-related differences in human lacrimal fluid peroxidase activity. Ophthalmologica. 2003;217:294-297.
39. Saari KM, Aho V, Paavilainen V, Nevalainen TJ. Group II PLA(2) content of tears in normal subjects. Invest Ophthalmol Vis Sci. 2001;42:318-320.
40. Sack RA, Tan KO, Tan A. Diurnal tear cycle: Evidence for a nocturnal inflammatory constitutive tear fluid. Invest Ophthalmol Vis Sci. 1992;33:626-640.