Plasmid profile analysis of multidrug resistant pseudomonas aeruginosa isolated from wound infections in South West, Nigeria

World Applied Sciences Journal

Volumn 20, Issue 6, 2012, Pages 766-775

  Akingbade, O A ^a,b   Balogun, S A ^b   Ojo, D A ^b   Afolabi, R O ^b   Motayo, B O ^c   Okerentugba, P O ^d   Okonko, I O ^d  

^a FEDERAL MEDICAL CENTER   (Nigeria)

^b FEDERAL UNIVERSITY OF AGRICULTURE   (Nigeria)

^c UNIVERSITY OF IBADAN   (Nigeria)

^d UNIVERSITY OF PORT HARCOURT   (Nigeria)

Author keywords

Antibiotics; Electrophoresis; Multi drug resistance (MDR); Nigeria; P. aeruginosa; Plasmid profile

Indexed keywords

EID: 84871853072     PISSN: 18184952     EISSN: 19916426     Source Type: Journal    
DOI: 10.5829/idosi.wasj.2012.20.06.667     Document Type: Article

References (55)

1. Bojary, N.M.R. and M. Hajia, 2012. Multidrug- resistant Pseudomonas aeruginosa strains in Tehran Reference Burn Hospital, Tehran, Iran. African Journal of Microbiology Research, 6: 1393-1396.
2. Hajia, M., M. Qorbanalizadehgan, M. Rahbar and M. Izadi, 2008. Laboratory Evaluation of Iranian Commercially Provided Antibiotic Disks With Conventional E-Test for Susceptibility Testing in Three Most Isolated Multi-drug Resistant Organisms. Internet J. Mic., 5(1).
3. Rahbar, M., H. Mehragan and N. Hajiali-Akbari, 2010. Prevalence of Drug Resistance in Nonfermenter Gram-Negative Bacilli. Iranian J. Path., 5: 90-96.
4. Obritsch, M.D., D.N. Fish, R. McLaren and R. Jung, 2004. National Surveillance of Antimicrobial Resistance in Pseudomonas aeruginosa isolates obtained from Intensive Care Unit Patients from 1993 to 2002. Antimicrob Agents Chemother, 48: 4606-4610.
5. Mulu, A., F. Moges, B. Tesema and A. Kassu, 2006. Patterns and multiple drug resistance of bacterial pathogens at university of Gondar teaching hospital, Northwest Ethiopia. Ethiopian Medical Journal, 44(2): 125-131.
6. Agarwal, G., A. Kapil, S.K. Kabra, B.K. Das and N. Dwivedi, 2005. Characterization of Pseudomonas aeruginosa isolated from chronically infected children with cystic fibrosis in India. BMC Microbiology, 5: 43.
7. Micek, S.T., A.E. Lloyd, D.J. Ritchie, R.M. Reichley, V.J. Fraser and M.H. Kollef, 2005. Pseudomonas aeruginosa bloodstream infection: importance of appropriate initial antimicrobial treatment. Antimicrob Agents Chemother, 49: 1306-1311.
8. Li, X.Z., D.M. Livermore and H. Nikaido, 1994. Role of efflux pump(s) in intrinsic resistance of Pseudomonas aeruginosa: resistance to tetracycline, chloramphenicol and norfloxacin. Antimicrob Agents Chemother, 38(8): 1732-1741.
9. Livermore, D.M., 2004. Multiple mechanisms of antimicrobial resistance in Ps. aeruginosa: Our worst nightmare? CID. Antimicrobial Resistance, 34: 634-664.
10. Bonomo, R.A. and D. Szabo, 2006. Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa. Clin. Infect. Dis., 43: S49-56.
11. Weldhagen, G.F., L. Poirel and P. Nordmann, 2003. Ambler class A extended-spectrum. lactamases in Pseudomonas aeruginosa: novel developments and clinical impact. Antimicrob Agents Chemother, 47: 2385-2392.
12. Walsh, T.R., M.A. Toleman, L. Poirel and P. Nordmann, 2005. Metallo-b-lactamases: the quiet before storm? Clin Microbiol Rev., 18: 306-325.
13. Shadid, M. and A. Malik, 2004. Plasmid mediated Amikacin Resistance in clinical isolates of Pseudomonas aeruginosa. India J. Med. Microb., 22: 182-184.
14. Odusanya, O.O., 2002. Antibiotic susceptibility of microorganisms at a General Hospital in Lagos, Nigeria. J Natl Med Association, 94: 994-998.
15. Smith, S., O. Ganiyu, R. John, M. Fowora, K. Akinsinde and P. Odeigah, 2012. Antimicrobial Resistance and Molecular Typing of Pseudomonas aeruginosa Isolated from Surgical Wounds in Lagos, Nigeria. Acta Medica Iranica, 50: 433-438.
16. Jombo, G.T., P. Jonah and J.A. Ayeni, 2008. Multiple resistant Pseudomonas aeruginosa in contemporary medical practice:findings fromurinary isolates at a Nigerian University Teaching Hospital. Nigerian Journal Physio Science, 23: 105-109.
17. Wariso, B.A. and S.N. Ibe, 2006. Bacteriology of chronic discharging ears in Port Harcourt, Nigeria. West African Journal of Medicine, 25: 219-222.
18. Sazakli, E., M. Leotsinidis, A. Vantarakis and M. Papapetropoulou, 2005. Comparative typing of Pseudomonas species isolated from the aquatic environment in Greece by SDS-PAGE and RAPD analysis. Journal of Applied Microbiology, 99: 1191-1203.
19. Ogle, J.W., J.M. Janda, D.E. Woods and M.L. Vasil, 1987. Characterization and use of a DNA probe as an epidemiologic marker for Pseudomonas aeruginosa. J. Infect. Dis., 155: 119-126.
20. Cheesbrough, M., 2006. District laboratory practice in tropical countries. Part 2. Cambridge Unversity press; pp: 434.
21. Bauer, A.W., W.M. Kirby, J.C. Sherris and M. Turck, 1966. Antibiotic susceptibility testing by a standard single disc method. American Journal Clinical Pathology, 45: 493-496.
22. National Committee for Clinical Laboratory Standards (NCCLS), 2007. Performance standards for antimicrobial susceptibility testing: twelfth informational supplement. NCCLS document M100-S12. PA, USA.
23. Clinical and Laboratory Standards Institute (CLSI), 2008. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; Approved standard-Third edition, CLSI document M31-A3, Clinical and Laboratory Standards Institute, 940 West Valley Road, Wayne Pennsylvania, USA, 28: 1-99.
24. Bikandi, J., R. San Millán, A. Rementeria and J. Garaizar, 2004. In silico analysis of complete bacterial genomes: PCR AFLP-PCR and endonuclease restriction. Bioinformatics, 20: 798-799.
25. Ogbolu, D.O., A. Ogunledun, O.E. Adebiyi, O.A. Daini and A.O. Alli, 2008. Antibiotic susceptibility patterns of Pseudomonas aeruginosa to available antipseudomonal drugs in Ibadan, Nigeria. Afr J. Medical Science, 37: 339-344.
26. Daini, O.A., M.J. Effiong and O.D. Ogbolu, 2008. Quinolones Resistance and R-Plasmids of Clinical Isolates of Pseudomonas species. Sudan J. M. Sci., 3: 139-146.
27. Olayinka, A.T., B.O. Olayinka and B.A. Onile, 2009. Antibiotic Susceptibility and Plasmid pattern of Pseudomonas aeruginosa from the surgical unit of a University Teaching Hospital in North Central, Nigeria. Int. J. Med. Med. Sci., 1: 79-83.
28. Yimaz, A., A.Y. Cobian, Y. Yelz, C. Tamverdi, T.T. Yidrim, Z.Z. Erturan, B.B. Dunipnar and B.B. Bozdogan, 2011. Investigation of Plasmid- Mediated quinolone Resistance in Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Microbiol. Bull., 45: 602-608.
29. Daini, O.A. and C.G. Charles-Onyeaghala, 2012. Plasmid- mediated aminoglycoside resistance of clinical isolates of pseudomonas aeruginosa. Global Advanced Research Journal of Microbiology, 1: 052-056.
30. Van Eldere, J., 2003. Multicentre surveillance of Pseudomonas aeruginosa susceptibility patterns in nosocomial infections. Journal of Antimicrobial Chemotherapy, 51: 347-352.
31. Gales, A.C., R.N. Jones, J. Turnidge, R. Rennie and R. Ramphal, 2001. Characterization of Pseudomonas aeruginosa: occurrence rate, antimicrobial susceptibility pattern and molecular typing in the global sentry antimicrobial surveillance program 1997-1999. Clinic Infect Dis., 32: S146-155.
32. Yetkin, G., B. Otilu, A. Cicek, C. Kuzucu and R. Durmaz, 2006. Clinical microbiologic and epidemiologic characteristics of P. aeruginosa infections in a University Hospital, Malaya, Turkey. Am. J. Infect. Control, 34: 188-192.
33. Brown, P.D. and A. Izundu, 2004. Antibiotic resistance in clinical isolates of Pseudomonas aeruginosa in Jamaica. Pan Am. J. Public Health, 16: 125-130.
34. Müller-Premru, M. and M. Gubina, 2000. Serotype, antimicrobial susceptibility and clone distribution of Pseudomonas aeruginosa in a university hospital. Zentralbl Bakteriol., 289: 857-867.
35. Jonas, R.N., 2001. Resistance patterns among nosocomial pathogens: trends over the past few years. Chest., 119: 397s-404s.
36. Bouza, E., F. Garcia-Gorrote, E. Cercenado, M. Marin and M.S. Diaz, 1999. Pseudomonas aeruginosa: a survey of resistance in 136 hospitals in Spain. The Spanish Pseudomonas aeruginosa study group. Antimicrob Agents Chemother., 43: 981-982.
37. Bonfiglio, G., V. Carciotto, G. Russo, S. Stefani, G.C. Schito, E. Debbia and G. Nicoletti, 1998. Antibiotic resistance in Pseudomonas aeruginosa: an Italian survey. J Antimicrob Chemother., 41: 307-310.
38. Spencer, R.C., 1996. An 8-year microbe base survey of the epidemiology, frequency and antibiotic susceptibility of Pseudomonas aeruginosa hospital isolates in the United Kingdom. J. Antimicrob Chemother., 37: 295-301.
39. Algun, U., A. Arisoy, T. Gunduz and B. Ozbakkaloglu, 2004. The resistance of Ps. aeruginosa strains to fluoroquinolone group of antibiotics. Indian J. Med. Microbiol., 22: 112-114.
40. Enabulele, I.O., S.C. Yah, E.O. Yusuf and N.O. Eghafona, 2006. Emerging quinolone resistant transfer genes among Gram-negative bacteria, isolated from faeces of HIV/AIDS patients attending some Clinics and Hospitals in the City of Benin, Edo State, Nigeria. Online Journal Health and Allied Science, 3(3) http://www.ojhas.org/issue19/2006-3-3.htm.
41. Anupurba, S., A. Bhattacharjee, A. Garg and R. Sen Malay, 2006. Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from wound infections. Indian Journal of Dermatology, 51: 286-288.
42. Oguntibeju, O.O. and R.A. Nwobu, 2004. Occurrence of P. aeruginosa in post operative wound infection. Pakistan Journal of Medical Sciences, 20: 187-191.
43. Nwanze, P.I., L.M. Nwaru, S. Oranusi, U. Dimkpa, M.U. Okwu, B.B. Babatunde, T.A. Anake, W. Jatto and C.E. Asagwara, 2007. Urinary tract infection in Okada village: Prevalence and antimicrobial susceptibility pattern. Scientific Research and Essay, 2: 112-116.
44. Idu, F.K. and S.E. Odjimogho, 2003. Susceptibility of conjunctival bacterial pathogens to flouroquinolones: A comparative study of ciprofloxacin, norfloxacin and Ofloxacin. Online Journal of Health and Allied Sciences, (OJHAS): 2(3).
45. Nikbin, V.S., A. Abdi-Ali, M.M. Feizabadi and S. Gharavi, 2007. Pulsed-field gel electrophoresis and plasmid profile of Pseudomonas aeruginosa at two hospitals in Tehran, Iran. Indian J. Med. Res., 126: 146-151.
46. Tassios, P.T., V. Gennimata, A.N. Maniatis, C. Fock, N.J. Legakis and the Greek Pseudomonas aeruginosa Study Group, 1998. Emergence of multidrug resistance in ubiquitous and dominant Pseudomonas aeruginosa serogroup 0:11. J. Clin. Microbiol., 36: 897-901.
47. Goswami, N.N., H.R. Trivedi, A.P.P. Goswami, T.K. Patel and C.B. Tripathi, 2011. Antibiotic sensitivity profile of bacterial pathogens in postoperative wound infections at a tertiary care hospital in Gujarat, India. J. Pharmacol Pharmacother; 2: 158-164.
48. Fadeyi, A., A.A. Akanbi 2nd, C. Nwabuisi and B.A. Onile, 2005. Antibiotic disc sensitivity pattern of Pseudomonas aeruginosa isolates obtained from clinical specimen in Ilorin, Nigeria. Afr. J. Med., 34: 303-306.
49. Raja, N.S. and N.N. Singh, 2007. Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in tertiary care hospital. J. Microbiol Immunol Infect., 40: 45-49.
50. rd ed. Hyde Park, VT: Antimicrobial Therapy Inc.
51. Rotimi, A.O., E.O. Esho and P.A. Emina, 1984. Outbreak of multiple - resistant in Pseudomonas aeruginosa carrying transferable resistance factor (R plasmids) in a urology clinic. Niger. Qrt. J. Hosp. Med., 2: 3-9.
52. Tsakris, A., A.C. Vatopoulous, L.S. Tzouvelekis and N.J. Legakis, 1992. Diversity of resistant phenotypes and plasmid analysis in multi resistant 0:12 Ps. aeruginosa. Eur. J. Epidemiol., 8: 865-780.
53. Olayinka, B.O., O.S. Olonitola, A.T. Olayinka and E.A. Agada, 2004. Antibiotic susceptibility pattern and multiple antibiotic resistance (MAR) index of Pseudomonas aeruginosa urine isolates from a University Teaching Hospital. African Journal of Clinical and Experimental Microbiology, 5: 198-202.
54. Onipede, A.O., A.A. Onayade, J.B.E. Elusiyan, P.O. Obiajunwa, E.O.O. Ogundare, O.O. Olaniran, L.A. Adeyemi and O.O. Oyelami, 2009. Invasive bacteria isolates from children with severe infections in a Nigerian hospital. J. Infect Develop Countries, 3: 429-436.
55. Yoon, M.Y., K. Lee, S.H. Jeong, J. Kim and S.S. Yoon, 2010. Heterogeneous virulence potential and high antibiotic resistance of Pseudomonas aeruginosa strains isolated from Korean pneumonia patients. J. Microbiol., 48: 518-525.