Juxtacellular neuronal labelling, physiological characterization and phenotypic identification of single neurons in vivo

Neuromethods

Volumn 78, Issue , 2013, Pages 167-186

  Verberne, Anthony J M ^a   Llewellyn Smith, Ida J ^b  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b FLINDERS UNIVERSITY   (Australia)

Author keywords

Electrophysiology; Extracellular; Immunohistochemistry; Neurochemistry; Ultrastructure

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN REGION; CELL LABELING; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; IN VIVO STUDY; INTRACELLULAR RECORDING; JUXTACELLULAR NEURONAL LABELING; MICROSCOPY; NERVE CELL; NERVE FUNCTION; NEUROCHEMISTRY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT;

EID: 84871828680     PISSN: 08932336     EISSN: 19406045     Source Type: Book Series    
DOI: 10.1007/978-1-62703-233-9_10     Document Type: Article

References (23)

1. Humphrey DR, Schmidt EM (1990) Extracellular single-unit recording methods. In: Boulton AA, Baker GB, Vanderwolf CH (eds) Neuromethods. Neurophysiological techniques: application to neural systems. Humana Press, Clifton, NJ, pp 1-64
2. Lipski J (1981) Antidromic activation of neurones as an analytical tool in the study of the central nervous system. J Neurosci Methods 4 :1-32
3. Duque A, Zaborszky L (2006) Juxtacellular labeling of individual neurons in vivo: from electrophysiology to synaptology. In: Zaborszky L, Wouterlood FG, Lanciego JL (eds) Neuroanatomical tract-tracing. Molecules, neurons and systems. Springer, New York, pp 197-236
4. Pinault D (1994) Golgi-like labeling of a single neuron recorded extracellularly. Neurosci Lett 170 :255-260
5. Pinault D (1996) A novel single-cell staining procedure performed in vivo under electrophysiological control: morpho-functional features of juxtacellularly labeled thalamic cells and other central neurons with biocytin or neurobiotin. J Neurosci Methods 65 :113-136
6. Kitai ST, Penny GR, Chang HT (1989) Intracellular labeling and immunocytochemistry. In: Heimer L, Zaborsky L (eds) Neuroanatomical tract-tracing methods. Recent progress. Plenum Press, New York, pp 173-199
7. Armstrong WE (1992) Intracellular recording and labelling with biocytin- fi lled electrodes. In: Kettenmann H, Grantyn R (eds) Practical electrophysiological methods. A guide for in vitro studies in vertebrate neurobiology. Wiley-Liss Inc., New York, pp 304-308
8. Schreihofer AM, Guyenet PG (2003) Baroactivated neurons with pulse-modulated activity in the rat caudal ventrolateral medulla express GAD67 mRNA. J Neurophysiol 89 :1265-1277
9. Schreihofer AM, Guyenet PG (1997) Identification of C1 presympathetic neurons in rat rostral ventrolateral medulla by juxtacellular labeling in vivo. J Comp Neurol 387 :524-536
10. Rouzade-Dominguez ML, Pernar L, Beck S, Valentino RJ (2003) Convergent responses of Barrington's nucleus neurons to pelvic visceral stimuli in the rat: a juxtacellular labelling study. Eur J Neurosci 18 :3325-3334
11. Verberne AJM, Stornetta RL, Guyenet PG (1999) Properties of C1 and other ventrolateral medullary neurones with hypothalamic projections in the rat. J Physiol (Lond) 517 :477-494
12. Kirouac GJ, Pittman QJ (1999) Identification of barosensitive neurons in the mediobasal forebrain using juxtacellular labeling. Am J Physiol 276:R1766-R1771
13. Schreihofer AM, Stornetta RL, Guyenet PG (1999) Evidence for glycinergic respiratory neurons: Botzinger neurons express mRNA for glycinergic transporter 2. J Comp Neurol 407 :583-597
14. Houweling AR, Doron G, Voigt BC, Herfst LJ, Brecht M (2010) Nanostimulation: manipulation of single neuron activity by juxtacellular current injection. J Neurophysiol 103 :1696-1704
15. Jones GA, Llewellyn-Smith IJ, Jordan D (2002) Physiological, pharmacological, and immunohistochemical characterisation of juxtacellularly labelled neurones in rat nucleus tractus solitarius. Auton Neurosci 98 :12-16
16. Bangasser DA, Zhang X, Garachh V, Hanhauser E, Valentino RJ (2011) Sexual dimorphism in locus coeruleus dendritic morphology: a structural basis for sex differences in emotional arousal. Physiol Behav 103 :342-351
17. Houweling AR, Brecht M (2008) Behavioural report of single neuron stimulation in somatosensory cortex. Nature 451 :65-68
18. Andrew RD, Fagan M (1990) A technique for controlling the membrane potential of neurons during unit recording. J Neurosci Methods 33 :55-60
19. Sartor DM, Verberne AJM (2003) Phenotypic identification of rat rostroventrolateral medullary presympathetic neurons inhibited by exogenous cholecystokinin. J Comp Neurol 465 :467-479
20. Llewellyn-Smith IJ, Dicarlo SE, Collins HL, Keast JR (2005) Enkephalin-immunoreactive interneurons extensively innervate sympathetic preganglionic neurons regulating the pelvic viscera. J Comp Neurol 488 :278-289
21. Llewellyn-Smith IJ, Schreihofer AM, Guyenet PG (2001) Distribution and amino acid content of enkephalin-immunoreactive inputs onto juxtacellularly labelled bulbospinal barosensitive neurons in rat rostral ventrolateral medulla. Neuroscience 108 :307-322
22. Llewellyn-Smith IJ, Minson JB (1992) Complete penetration of antibodies into vibratome sections after glutaraldehyde fi xation and ethanol treatment: light and electron microscopy for neuropeptides. J Histochem Cytochem 40 :1741-1749
23. Burman KJ, Sartor DM, Verberne AJM, Llewellyn-Smith IJ (2004) Cocaine- and amphetamine-regulated transcript in catecholamine and non-catecholamine bulbospinal, barosensitive neurons of the rat rostral ventrolateral medulla. J Comp Neurol 476 :19-31